5(6)-FAM SE (Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

Cat. No.: HY-15937 Purity: 95.42%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.

For research use only. We do not sell to patients.

5(6)-FAM SE Chemical Structure

CAS No. : 117548-22-8

Size	Price	Stock	Quantity
5 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 144	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.

Molecular Weight

473.39

Formula

C₂₅H₁₅NO₉

CAS No.

117548-22-8

Appearance

Solid

Color

Yellow to orange

Emission (Em)

515

Excitation (Ex)

488

SMILES

OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (176.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1124 mL	10.5621 mL	21.1242 mL
5 mM	0.4225 mL	2.1124 mL	4.2248 mL
10 mM	0.2112 mL	1.0562 mL	2.1124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 6.25 mg/mL (13.20 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 6.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.39 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.42%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (256 KB) HNMR (189 KB) LCMS (401 KB)

Handling Instructions (2659 KB)

References

[1]. Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91. [Content Brief]

[2]. Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1124 mL	10.5621 mL	21.1242 mL	52.8106 mL
	5 mM	0.4225 mL	2.1124 mL	4.2248 mL	10.5621 mL
	10 mM	0.2112 mL	1.0562 mL	2.1124 mL	5.2811 mL
	15 mM	0.1408 mL	0.7041 mL	1.4083 mL	3.5207 mL
	20 mM	0.1056 mL	0.5281 mL	1.0562 mL	2.6405 mL
	25 mM	0.0845 mL	0.4225 mL	0.8450 mL	2.1124 mL
	30 mM	0.0704 mL	0.3521 mL	0.7041 mL	1.7604 mL
	40 mM	0.0528 mL	0.2641 mL	0.5281 mL	1.3203 mL
	50 mM	0.0422 mL	0.2112 mL	0.4225 mL	1.0562 mL
	60 mM	0.0352 mL	0.1760 mL	0.3521 mL	0.8802 mL
	80 mM	0.0264 mL	0.1320 mL	0.2641 mL	0.6601 mL
	100 mM	0.0211 mL	0.1056 mL	0.2112 mL	0.5281 mL

5(6)-FAM SE Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5(6)-FAM SE117548-22-85(6)-Carboxyfluorescein N-hydroxysuccinimide ester 5(6)-Carboxyfluorescein succinimidyl ester mixed isomersFluorescent DyeInhibitorinhibitorinhibit

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