6-Aminofluorescein (Synonyms: 6-AF)

Cat. No.: HY-D0022A Purity: 98.84%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

6-Aminofluorescein (6-AF) is a new fluorescence marker.

For research use only. We do not sell to patients.

6-Aminofluorescein Chemical Structure

CAS No. : 51649-83-3

Size	Price	Stock	Quantity
50 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 51	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
1 g	USD 120	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Other Forms of 6-Aminofluorescein:

5-Aminofluorescein In-stock

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Description

6-Aminofluorescein (6-AF) is a new fluorescence marker.

Molecular Weight

347.32

Formula

C₂₀H₁₃NO₅

CAS No.

51649-83-3

Appearance

Solid

Color

Yellow to orange

Emission (Em)

515

Excitation (Ex)

490

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(N)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (287.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8792 mL	14.3959 mL	28.7919 mL
5 mM	0.5758 mL	2.8792 mL	5.7584 mL
10 mM	0.2879 mL	1.4396 mL	2.8792 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.84%

Select Batch:

Data Sheet (248 KB) SDS (251 KB)

COA (256 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8792 mL	14.3959 mL	28.7919 mL	71.9797 mL
	5 mM	0.5758 mL	2.8792 mL	5.7584 mL	14.3959 mL
	10 mM	0.2879 mL	1.4396 mL	2.8792 mL	7.1980 mL
	15 mM	0.1919 mL	0.9597 mL	1.9195 mL	4.7986 mL
	20 mM	0.1440 mL	0.7198 mL	1.4396 mL	3.5990 mL
	25 mM	0.1152 mL	0.5758 mL	1.1517 mL	2.8792 mL
	30 mM	0.0960 mL	0.4799 mL	0.9597 mL	2.3993 mL
	40 mM	0.0720 mL	0.3599 mL	0.7198 mL	1.7995 mL
	50 mM	0.0576 mL	0.2879 mL	0.5758 mL	1.4396 mL
	60 mM	0.0480 mL	0.2399 mL	0.4799 mL	1.1997 mL
	80 mM	0.0360 mL	0.1799 mL	0.3599 mL	0.8997 mL
	100 mM	0.0288 mL	0.1440 mL	0.2879 mL	0.7198 mL

6-Aminofluorescein Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Aminofluorescein51649-83-36-AFFluorescent DyeInhibitorinhibitorinhibit

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