6-Methylcoumarin

Name: 6-Methylcoumarin
Brand: MedChemExpress
SKU: HY-N1406
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1406 Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

6-Methylcoumarin is a synthetic fragrance widely used in cosmetics.

For research use only. We do not sell to patients.

6-Methylcoumarin Chemical Structure

CAS No. : 92-48-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 60	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 6-Methylcoumarin:

6-Methylcoumarin (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

6-Methylcoumarin is a synthetic fragrance widely used in cosmetics.

In Vitro

6-Methylcoumarin is reported to be almost nonphototoxic in epidermal tissue and cell culture phototoxicity test models. HaCaT cells are treated with 6-Methylcoumarin (1-10⁵ nM) and/or UVA (5 J cm^-2), and γ-H2AX is detected by immunofluorescence staining and western blotting 4hours after the treatments. 6-Methylcoumarin clearly produces γ-H2AX-positive cells under UVA irradiation from a concentration of 100 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

160.17

Formula

C₁₀H₈O₂

CAS No.

92-48-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=CC2=CC(C)=CC=C2O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (312.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.2434 mL	31.2168 mL	62.4337 mL
5 mM	1.2487 mL	6.2434 mL	12.4867 mL
10 mM	0.6243 mL	3.1217 mL	6.2434 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (247 KB) RP-HPLC (258 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Toyooka T, et al. Phosphorylation of histone H2AX is a powerful tool for detecting chemical photogenotoxicity. J Invest Dermatol. 2011 Jun;131(6):1313-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.2434 mL	31.2168 mL	62.4337 mL	156.0842 mL
	5 mM	1.2487 mL	6.2434 mL	12.4867 mL	31.2168 mL
	10 mM	0.6243 mL	3.1217 mL	6.2434 mL	15.6084 mL
	15 mM	0.4162 mL	2.0811 mL	4.1622 mL	10.4056 mL
	20 mM	0.3122 mL	1.5608 mL	3.1217 mL	7.8042 mL
	25 mM	0.2497 mL	1.2487 mL	2.4973 mL	6.2434 mL
	30 mM	0.2081 mL	1.0406 mL	2.0811 mL	5.2028 mL
	40 mM	0.1561 mL	0.7804 mL	1.5608 mL	3.9021 mL
	50 mM	0.1249 mL	0.6243 mL	1.2487 mL	3.1217 mL
	60 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6014 mL
	80 mM	0.0780 mL	0.3902 mL	0.7804 mL	1.9511 mL
	100 mM	0.0624 mL	0.3122 mL	0.6243 mL	1.5608 mL