6-OAU (Synonyms: GTPL5846)

Name: 6-OAU
Brand: MedChemExpress
SKU: HY-12764
Rating: 4.5 (1 reviews)

Cat. No.: HY-12764 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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6-OAU (GTPL5846) (6-n-octylaminouracil) is an GPR84 (G protein-coupled receptor 84) agonist, with an EC₅₀ value of 105 nM. 6-OAU works as a chemoattractant to both PMNs and macrophages, and amplifies the proinflammatory cytokine IL-8, shows proinflammatory function. 6-OAU also displays anti-bacterial function.

For research use only. We do not sell to patients.

6-OAU Chemical Structure

CAS No. : 83797-69-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 526	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 526	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1800	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 6-OAU

•J Clin Invest. 2023 Oct 19:e168992. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

ERK

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.0167 μM Compound: 3	Agonist activity at recombinant human GPR84 expressed in CHO cells co-expressing beta-galactosidase/beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation measured after 15 mins by radioactive assay Agonist activity at recombinant human GPR84 expressed in CHO cells co-expressing beta-galactosidase/beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation measured after 15 mins by radioactive assay	[PMID: 31721581]
CHO	EC₅₀	0.114 μM Compound: 3	Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as beta-arrestin 2 recruitment measured after 90 mins by beta-galactosidase based PathHunter assay Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as beta-arrestin 2 recruitment measured after 90 mins by beta-galactosidase based PathHunter assay	[PMID: 31721581]
HEK293	EC₅₀	0.105 μM Compound: 3	Agonist activity at recombinant human GPR84 expressed in HEK293 cells co-expressing Gqi5 assessed as increase in myo-[3H]inositol phosphate accumulation measured after 2 hrs by topcount scintillation counting method Agonist activity at recombinant human GPR84 expressed in HEK293 cells co-expressing Gqi5 assessed as increase in myo-[3H]inositol phosphate accumulation measured after 2 hrs by topcount scintillation counting method	[PMID: 31721581]
HEK293	EC₅₀	0.341 μM Compound: 3	Agonist activity at recombinant human GPR84 stably expressed in HEK293 cells co-expressing Galpha16 assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by forskolin addition and measured after 30 mins by HTRF as Agonist activity at recombinant human GPR84 stably expressed in HEK293 cells co-expressing Galpha16 assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by forskolin addition and measured after 30 mins by HTRF as	[PMID: 31721581]
HEK293	EC₅₀	0.653 μM Compound: 6-OAU	Agonist activity at GPR84 (unknown origin) expressed in HEK293 cells coexpressing Galpha16 protein by Fluo-4 AM dye based calcium mobilization assay Agonist activity at GPR84 (unknown origin) expressed in HEK293 cells coexpressing Galpha16 protein by Fluo-4 AM dye based calcium mobilization assay	[PMID: 27326330]
HEK293	EC₅₀	1.25 μM Compound: 3	Agonist activity at recombinant human GPR84 stably expressed in HEK293 cells co-expressing Galpha16 assessed as increase in intracellular calcium mobilization by Fluo-4 AM dye based fluorescence assay Agonist activity at recombinant human GPR84 stably expressed in HEK293 cells co-expressing Galpha16 assessed as increase in intracellular calcium mobilization by Fluo-4 AM dye based fluorescence assay	[PMID: 31721581]
HEK293	EC₅₀	1.75 μM Compound: 3	Agonist activity at recombinant human GPR84 expressed in HEK293 cells assessed as beta-arrestin 2 recruitment measured after 20 mins by luciferase reporter gene assay Agonist activity at recombinant human GPR84 expressed in HEK293 cells assessed as beta-arrestin 2 recruitment measured after 20 mins by luciferase reporter gene assay	[PMID: 31721581]
HEK293	EC₅₀	438 nM Compound: 6-OAU	Agonist activity at GPR84 (unknown origin) expressed in in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forskolin addition measured after 30 mins by HTRF assay Agonist activity at GPR84 (unknown origin) expressed in in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forskolin addition measured after 30 mins by HTRF assay	[PMID: 27326330]
Sf9	EC₅₀	0.512 μM Compound: 3	Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 1 hr by liquid scintillation counting method Agonist activity at human Gi1alpha-coupled GPR84 expressed in baculovirus infected Sf9 insect cells assessed as stimulation of [35S]GTPgammaS binding measured after 1 hr by liquid scintillation counting method	[PMID: 31721581]
Sf9	EC₅₀	512 nM Compound: 6-OAU	Agonist activity at human GPR84 fused with bovine Galphai1 protein expressed in baculovirus infected sf9 cell membrane incubated for 1 hr by [35S]GTPgammaS binding assay Agonist activity at human GPR84 fused with bovine Galphai1 protein expressed in baculovirus infected sf9 cell membrane incubated for 1 hr by [35S]GTPgammaS binding assay	[PMID: 27326330]

In Vitro

GPR84 gene exhibits high expression in human polymorphonuclear leukocytes (PMNs) and macrophages, 6-OAU acts on proinflammatory function by activitating GPR84^[1].
6-OAU (0.01 nM-0.1 mM; 1 h) activates human GPR84 in the presence of G_qi5 chimera with an EC₅₀ value of 105 nM in HEK293 cells^[1].
6-OAU (0, 6.25, 200 μM; 30 min) stimulates [³⁵S]GTP binding, accumulates phosphoinositides, and induces GPR84-EGFP internalization in a GPR84-dependent manner^[1].
6-OAU (1 nM-1 mM; 1 h) provokes chemotaxis of PMNs in a concentration-dependent manner with an EC₅₀ value of 318 nM^[1].
6-OAU (0-10 μM; 4 h) increases the secretion of IL-8 from LPS-stimulated PMNs^[1].
6-OAU (0-0.4 μM; 16 h) also amplifies TNF-α production from U937 macrophages^[1].
6-OAU (2 μM; 4 h) decreases ERK phosphorylation and MCP-1 protein expression, (2 μM; 48 h) decreases MCP-1 secretion in macrophages ^[2].
6-OAU (2 μM; 24 h) reduces ROS production during B. abortus infection in RAW264.7 cells^[2].
6-OAU (2 μM; 0, 30, and 60 min) inhibits adhesion and Brucella uptake in RAW264.7 cells and (2 μM; 30 min) shows anti-infection against Brucella and Salmonella infection^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	B. abortus
Concentration:	0, 0.02, 0.2, 2 μM
Incubation Time:	0, 2, 24, 48, 72 hours
Result:	Decreased B. abortus survivability begin at 48 h with a dose of 2 μM.

Western Blot Analysis^[2]

Cell Line:	RAW264.7 cells infected with B. abortus
Concentration:	2 μM
Incubation Time:	4 hours
Result:	Reduced ERK phosphorylation and MALT1 expression in RAW264.7 macrophages.

In Vivo

6-OAU activates GPR84 and results in making an inflammatory condition through chemokine production and chemotaxis in vivo^[1].
6-OAU (10 mg/kg; i.v.) raises the blood CXCL1 level in rats^[1].
6-OAU (1 mg/mL; s.c.) attracts both PMNs and macrophages into the air pouch^[1].
6-OAU (2 μM, 100 mL/mouse; s.c.) augments resistance to Brucella infection, and reduces bacterial proliferation in spleens and livers^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Lewis rats(4-week-old)^[1]
Dosage:	10 mg/kg
Administration:	Intravenous injection; collecting blood 3 h after injection
Result:	Increased the elevation of a chemokine, CXCL1 concentration in the serum peaking at 3 h after the injection.

Animal Model:	Rat air pouch model (4-week-old female rats)^[1]
Dosage:	1 mg/mL (PBS)
Administration:	Subcutaneous injection; washing the cavity 4 h after injection
Result:	Attracted both PMNs and macrophages into the air pouch, peaking at 4 h after the injection.

Animal Model:	ICR female mice (7-week-old)^[2]
Dosage:	2 μM (100 μL/mouse)
Administration:	Oral average; 7 days and another 14 days after treated mouse with B. abortus (2 × 105 CFU /100 μL; i.p.)
Result:	Reduced bacterial proliferation in the liver and spleen, and decreased IFN-γ but augmented IL-6 serum level. Lowed splenic weight of mice and splenic proliferation.

Molecular Weight

239.31

Formula

C₁₂H₂₁N₃O₂

CAS No.

83797-69-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC(C=C(NCCCCCCCC)N1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (104.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1787 mL	20.8934 mL	41.7868 mL
5 mM	0.8357 mL	4.1787 mL	8.3574 mL
10 mM	0.4179 mL	2.0893 mL	4.1787 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Purity & Documentation

Purity: 99.91%

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Data Sheet (276 KB) SDS (393 KB)

COA (241 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Suzuki M, et al. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor. J Biol Chem. 2013 Apr 12;288(15):10684-91. [Content Brief]

[2]. Reyes AWB, et al. Immune-metabolic receptor GPR84 surrogate and endogenous agonists, 6-OAU and lauric acid, alter Brucella abortus 544 infection in both in vitro and in vivo systems. Microb Pathog. 2021 Sep. 158:105079. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1787 mL	20.8934 mL	41.7868 mL	104.4670 mL
	5 mM	0.8357 mL	4.1787 mL	8.3574 mL	20.8934 mL
	10 mM	0.4179 mL	2.0893 mL	4.1787 mL	10.4467 mL
	15 mM	0.2786 mL	1.3929 mL	2.7858 mL	6.9645 mL
	20 mM	0.2089 mL	1.0447 mL	2.0893 mL	5.2234 mL
	25 mM	0.1671 mL	0.8357 mL	1.6715 mL	4.1787 mL
	30 mM	0.1393 mL	0.6964 mL	1.3929 mL	3.4822 mL
	40 mM	0.1045 mL	0.5223 mL	1.0447 mL	2.6117 mL
	50 mM	0.0836 mL	0.4179 mL	0.8357 mL	2.0893 mL
	60 mM	0.0696 mL	0.3482 mL	0.6964 mL	1.7411 mL
	80 mM	0.0522 mL	0.2612 mL	0.5223 mL	1.3058 mL
	100 mM	0.0418 mL	0.2089 mL	0.4179 mL	1.0447 mL

6-OAU Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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6-OAU (Synonyms: GTPL5846)

Customer Review

6-OAU Related Antibodies

1 Publications Citing Use of MCE 6-OAU

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6-OAU Related Antibodies

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Complete Stock Solution Preparation Table

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