2. GPCR/G Protein Stem Cell/Wnt MAPK/ERK Pathway Anti-infection
  3. GPR84 ERK Bacterial Antibiotic
  4. 6-OAU

6-OAU (GTPL5846) (6-n-octylaminouracil) is an GPR84 (G protein-coupled receptor 84) agonist, with an EC50 value of 105 nM. 6-OAU works as a chemoattractant to both PMNs and macrophages, and amplifies the proinflammatory cytokine IL-8, shows proinflammatory function. 6-OAU also displays anti-bacterial function.

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6-OAU Chemical Structure

6-OAU Chemical Structure

CAS No. : 83797-69-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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5 mg USD 420 In-stock
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Based on 1 publication(s) in Google Scholar

6-OAU Related Antibodies

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1 Publications Citing Use of MCE 6-OAU

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Description

6-OAU (GTPL5846) (6-n-octylaminouracil) is an GPR84 (G protein-coupled receptor 84) agonist, with an EC50 value of 105 nM. 6-OAU works as a chemoattractant to both PMNs and macrophages, and amplifies the proinflammatory cytokine IL-8, shows proinflammatory function. 6-OAU also displays anti-bacterial function[1][2].

IC50 & Target

ERK

 

In Vitro

GPR84 gene exhibits high expression in human polymorphonuclear leukocytes (PMNs) and macrophages, 6-OAU acts on proinflammatory function by activitating GPR84[1].
6-OAU (0.01 nM-0.1 mM; 1 h) activates human GPR84 in the presence of Gqi5 chimera with an EC50 value of 105 nM in HEK293 cells[1].
6-OAU (0, 6.25, 200 μM; 30 min) stimulates [35S]GTP binding, accumulates phosphoinositides, and induces GPR84-EGFP internalization in a GPR84-dependent manner[1].
6-OAU (1 nM-1 mM; 1 h) provokes chemotaxis of PMNs in a concentration-dependent manner with an EC50 value of 318 nM[1].
6-OAU (0-10 μM; 4 h) increases the secretion of IL-8 from LPS-stimulated PMNs[1].
6-OAU (0-0.4 μM; 16 h) also amplifies TNF-α production from U937 macrophages[1].
6-OAU (2 μM; 4 h) decreases ERK phosphorylation and MCP-1 protein expression, (2 μM; 48 h) decreases MCP-1 secretion in macrophages [2].
6-OAU (2 μM; 24 h) reduces ROS production during B. abortus infection in RAW264.7 cells[2].
6-OAU (2 μM; 0, 30, and 60 min) inhibits adhesion and Brucella uptake in RAW264.7 cells and (2 μM; 30 min) shows anti-infection against Brucella and Salmonella infection[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-OAU Related Antibodies

Cell Viability Assay[2]

Cell Line: B. abortus
Concentration: 0, 0.02, 0.2, 2 μM
Incubation Time: 0, 2, 24, 48, 72 hours
Result: Decreased B. abortus survivability begin at 48 h with a dose of 2 μM.

Western Blot Analysis[2]

Cell Line: RAW264.7 cells infected with B. abortus
Concentration: 2 μM
Incubation Time: 4 hours
Result: Reduced ERK phosphorylation and MALT1 expression in RAW264.7 macrophages.
In Vivo

6-OAU activates GPR84 and results in making an inflammatory condition through chemokine production and chemotaxis in vivo[1].
6-OAU (10 mg/kg; i.v.) raises the blood CXCL1 level in rats[1].
6-OAU (1 mg/mL; s.c.) attracts both PMNs and macrophages into the air pouch[1].
6-OAU (2 μM, 100 mL/mouse; s.c.) augments resistance to Brucella infection, and reduces bacterial proliferation in spleens and livers[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Lewis rats(4-week-old)[1]
Dosage: 10 mg/kg
Administration: Intravenous injection; collecting blood 3 h after injection
Result: Increased the elevation of a chemokine, CXCL1 concentration in the serum peaking at 3 h after the injection.
Animal Model: Rat air pouch model (4-week-old female rats)[1]
Dosage: 1 mg/mL (PBS)
Administration: Subcutaneous injection; washing the cavity 4 h after injection
Result: Attracted both PMNs and macrophages into the air pouch, peaking at 4 h after the injection.
Animal Model: ICR female mice (7-week-old)[2]
Dosage: 2 μM (100 μL/mouse)
Administration: Oral average; 7 days and another 14 days after treated mouse with B. abortus (2 × 105 CFU /100 μL; i.p.)
Result: Reduced bacterial proliferation in the liver and spleen, and decreased IFN-γ but augmented IL-6 serum level.
Lowed splenic weight of mice and splenic proliferation.
Molecular Weight

239.31

Formula

C12H21N3O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC(C=C(NCCCCCCCC)N1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (104.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1787 mL 20.8934 mL 41.7868 mL
5 mM 0.8357 mL 4.1787 mL 8.3574 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.91%

COA (241 KB) LCMS (259 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1787 mL 20.8934 mL 41.7868 mL 104.4670 mL
5 mM 0.8357 mL 4.1787 mL 8.3574 mL 20.8934 mL
10 mM 0.4179 mL 2.0893 mL 4.1787 mL 10.4467 mL
15 mM 0.2786 mL 1.3929 mL 2.7858 mL 6.9645 mL
20 mM 0.2089 mL 1.0447 mL 2.0893 mL 5.2234 mL
25 mM 0.1671 mL 0.8357 mL 1.6715 mL 4.1787 mL
30 mM 0.1393 mL 0.6964 mL 1.3929 mL 3.4822 mL
40 mM 0.1045 mL 0.5223 mL 1.0447 mL 2.6117 mL
50 mM 0.0836 mL 0.4179 mL 0.8357 mL 2.0893 mL
60 mM 0.0696 mL 0.3482 mL 0.6964 mL 1.7411 mL
80 mM 0.0522 mL 0.2612 mL 0.5223 mL 1.3058 mL
100 mM 0.0418 mL 0.2089 mL 0.4179 mL 1.0447 mL
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6-OAU Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-OAU83797-69-7GTPL5846GTPL 5846GTPL-5846GPR84ERKBacterialAntibioticG protein coupled receptor 84Extracellular signal regulated kinasesG protein-coupled receptorproinflammatory cytokineIL-8TNF-αmacrophagePMNHEK293Sf9U937chemotaxisinflammationGTPγSrat air pouch modelB. abortusCytokinesLauric acidMAPKsInhibitorinhibitorinhibit

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