7,4'-Di-O-methylapigenin (Synonyms: 4',7-Dimethoxy-5-Hydroxyflavone)

Cat. No.: HY-N2144 Purity: 98.89%: FAQs
Data Sheet SDS COA Handling Instructions

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The compound 7,4'-Di-O-methylapigenin may be partly responsible for the reported antifungal activity of C.

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7,4'-Di-O-methylapigenin Chemical Structure

CAS No. : 5128-44-9

Size	Price	Stock	Quantity
2 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
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Purity & Documentation
References
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Description

The compound 7,4'-Di-O-methylapigenin may be partly responsible for the reported antifungal activity of C. zeyheri, and may serve as a potential source of lead compounds that can be developed as antifungal phytomedicines.And it also showed inhibition of the drug efflux pumps (with IC50 = 51.64 μg/ml). IC50:51.64 μg/ml(Candida albicans drug efflux pumps)[2] In vitro: The isolated 7,4'-Di-O-methylapigenin was further investigated for its inhibitory activity on ABC drug efflux pumps in C. albicans by monitoring an increase in ciprofloxacin, assessing the level of its accumulation, in response to reserpine. There was a higher accumulation of ciprofloxacin in Candida cells in the presence of 7,4'-Di-O-methylapigenin than with reserpine. The compound 7,4'-Di-O-methylapigenine demonstrated the activity in a dose-dependent manner with IC50 value of 51.64 μg/ml. These results support those obtained from synergism assays where by the underlying synergistic antifungal mechanisms could be due to blockage of ABC efflux pumps and increasing the susceptibility of Candida to miconazole.[2] In vivo: In searching for natural products as potential anti-inflammatory agents, 7,4'-Di-O-methylapigenin wasn't evaluated in vivo for its ability to inhibit acute inflammation.[1]

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
WI-38 VA13	IC₅₀	38.9 μg/mL Compound: 5-hydroxy-7,4'-dimethoxyflavone	Cytotoxicity against human VA13 cells after 48 hrs by WST8 assay Cytotoxicity against human VA13 cells after 48 hrs by WST8 assay	[PMID: 16309318]

Molecular Weight

298.29

Formula

C₁₇H₁₄O₅

CAS No.

5128-44-9

Appearance

Solid

Color

Light brown to yellow

SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=CC(OC)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (16.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3524 mL	16.7622 mL	33.5244 mL
5 mM	0.6705 mL	3.3524 mL	6.7049 mL
10 mM	0.3352 mL	1.6762 mL	3.3524 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.89%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (247 KB) HNMR (237 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Alvarez, M.E. et al.Phytochemical study and anti-inflammatory properties of Lampaya hieronymi Schum. ex Moldenke.Farmaco. Jun-Jul;55(6-7):502-5.doi:10.1016/S0014-827X(00)00067-7(2000). [Content Brief]

[2]. Mangoyi, R., Midiwo, J. & Mukanganyama, S. Isolation and characterization of an antifungal compound 5-hydroxy-7,4'-dimethoxyflavone from Combretum zeyheri. BMC complementary and alternative medicine 15, 405, doi:10.1186/s12906-015-0934-7 (2015). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3524 mL	16.7622 mL	33.5244 mL	83.8111 mL
	5 mM	0.6705 mL	3.3524 mL	6.7049 mL	16.7622 mL
	10 mM	0.3352 mL	1.6762 mL	3.3524 mL	8.3811 mL
	15 mM	0.2235 mL	1.1175 mL	2.2350 mL	5.5874 mL

7,4'-Di-O-methylapigenin Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7,4'-Di-O-methylapigenin5128-44-94',7-Dimethoxy-5-HydroxyflavoneEndogenous MetaboliteInhibitorinhibitorinhibit

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