2. Others Anti-infection
  3. Fluorescent Dye Antibiotic Fungal
  4. 7-Amino-4-methylcoumarin

7-Amino-4-methylcoumarin  (Synonyms: Coumarin 120; AMC)

Cat. No.: HY-D0027 Purity: 99.91%
COA Handling Instructions

Molarity Calculator

7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively.

For research use only. We do not sell to patients.

7-Amino-4-methylcoumarin Chemical Structure

7-Amino-4-methylcoumarin Chemical Structure

CAS No. : 26093-31-2

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
250 mg USD 33 In-stock
500 mg USD 58 In-stock
1 g USD 100 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 7-Amino-4-methylcoumarin:

7-Amino-4-methylcoumarin Related Antibodies

Top Publications Citing Use of Products

View All Antibiotic Isoform Specific Products:

View All Isoforms
Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively.[1][2][3][4]

In Vitro

7-Amino-4-methylcoumarin for Tissue Pathology Analysis[2]
(1) Original solution preparation: Dissolve 7-Amino-4-methylcoumarin in 10 mg/mL DMSO and store it at room temperature in the dark.
(2) Working solution preparation: Dissolve 25 μL of the 7-Amino-4-methylcoumarin stock solution and 2.5 mg of 2-pyridine borane in 50 mL of 1 mg/mL citric acid solution.
(3) Dewax and hydrate the slices first, and then oxidize them in 0.5% (w/v) periodic acid solution for 10 minutes.
(4) After rinsing with distilled water, stain the slices with the 7-Amino-4-methylcoumarin working solution for 10 minutes.
(5) Rinse again with distilled water, then counterstain the slices with Mayer's hematoxylin for 1 minute.
(6) Wash the slices under slightly warm (about 40°C) tap water for 5 minutes, then immerse them in a solution of Eosin Y diluted with 95% ethanol in a 3:1 ratio for 2 minutes.
(7) After rinsing with distilled water, dehydrate and clear the slices, then mount them with the non-fluorescent, anhydrous mounting medium Entellan New.
(8) Use an Axio Observer inverted microscope illuminated with LED (385 nm UV fluorescence), employing a 96 H & E filter set with BP390/40, BS420, and BP450/40 for bright field morphological analysis and UV excitation fluorescence signal analysis.
7-Amino-4-methylcoumarin for Enzyme Activity Detection[3]
(1) Cultivate red fungi and related actinomycetes on glucose yeast extract agar at 25°C for five days.
(2) Dissolve 5 mg of the enzyme to be tested in 200 μL N,N-dimethylformamide to make a 2×10-3 M solution. Dilute it to 10 mL with 0.05 M Tris buffer, adjusting the pH to 7.0. The substrate solution can be used immediately without sterilization or stored at -20°C for up to 3 months.
(3) Generate fluorescent 7-Amino-4-methylcoumarin from the conjugated substrate through enzyme-catalyzed hydrolysis. At an excitation wavelength of 380 nm, close to the strong 366 nm line of a mercury arc lamp, there is strong fluorescence emission at 460 nm, falling into the blue region.
(4) Lightly draw a 20 mm diameter circle with a pencil on Whatman No. 3 filter paper (about 90 mm) placed inside a standard 100 mm petri dish lid.
(5) Transfer the cultured red fungal and related actinomycetes inoculum into the circular area on the filter paper using a wooden toothpick. Inoculate 8-10 spots in each petri dish, pressing them into the paper, and then add a drop of substrate using an automatic pipette.
(6) Use the bottom of the petri dish as a lid, and incubate the reactants at 37°C for 10 minutes.
(7) Record a positive reaction when a strong light blue fluorescence is observed under UV light.
7-Amino-4-methylcoumarin for Cu2+ Detection[4]
(1) Dissolve 7-Amino-4-methylcoumarin and o-phenylenediamine (OPD) in 0.2M Tris-HCl buffer at pH 7.4, with concentrations of 50 μM and 50 mM respectively, adjusting the pH to 7-9.
(2) Prepare a copper sulfate storage solution by dilution to obtain different concentrations ranging from 0-300 μM.
(3) Mix 100 μL of the different concentrations of Cu2+ solution with AMC (20 μL, 50 μM), OPD (50 μL, 50 mM), and Tris-HCl (830 μL, 0.2 M, pH 7.4) thoroughly at 37°C for 2 minutes, then incubate the mixture in a water bath at 37°C for 2 hours. Record the changes in the fluorescence intensity ratios (F557/F438) using an F-4500 fluorescence spectrophotometer at an excitation wavelength of 360 nm.
(4) Maintain 20 μL of 50 μM AMC, 50 μL of 50 mM OPD, and 830 μL of 0.2 M Tris-HCl buffer (pH = 7.4) in a 2 mL centrifuge tube in a 37°C water bath for 2 minutes; then add 100 μL of different metal ions to the reaction solution and react at 37°C for 2 hours. Record the changes in the fluorescence intensity ratios using an F-4500 fluorescence spectrophotometer at an excitation wavelength of 360 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Amino-4-methylcoumarin Related Antibodies
Molecular Weight

175.18

Formula

C10H9NO2
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C1C=C(C)C2=CC=C(N)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (570.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7084 mL 28.5421 mL 57.0841 mL
5 mM 1.1417 mL 5.7084 mL 11.4168 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (14.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (5.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

COA (247 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.7084 mL 28.5421 mL 57.0841 mL 142.7104 mL
5 mM 1.1417 mL 5.7084 mL 11.4168 mL 28.5421 mL
10 mM 0.5708 mL 2.8542 mL 5.7084 mL 14.2710 mL
15 mM 0.3806 mL 1.9028 mL 3.8056 mL 9.5140 mL
20 mM 0.2854 mL 1.4271 mL 2.8542 mL 7.1355 mL
25 mM 0.2283 mL 1.1417 mL 2.2834 mL 5.7084 mL
30 mM 0.1903 mL 0.9514 mL 1.9028 mL 4.7570 mL
40 mM 0.1427 mL 0.7136 mL 1.4271 mL 3.5678 mL
50 mM 0.1142 mL 0.5708 mL 1.1417 mL 2.8542 mL
60 mM 0.0951 mL 0.4757 mL 0.9514 mL 2.3785 mL
80 mM 0.0714 mL 0.3568 mL 0.7136 mL 1.7839 mL
100 mM 0.0571 mL 0.2854 mL 0.5708 mL 1.4271 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

7-Amino-4-methylcoumarin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Amino-4-methylcoumarin26093-31-2Coumarin 120 AMCCoumarin120Coumarin-120Fluorescent DyeAntibioticFungalcoumarinsantimicrobialfluorescent probelaser dyeInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
7-Amino-4-methylcoumarin
Cat. No.:
HY-D0027
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.