7-Dimethylaminocoumarin-4-acetic acid

Name: 7-Dimethylaminocoumarin-4-acetic acid
Brand: MedChemExpress
SKU: HY-W040681
Rating: 4.5 (1 reviews)

Cat. No.: HY-W040681 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

7-Dimethylaminocoumarin-4-acetic acid is a fluorescent probe.

For research use only. We do not sell to patients.

7-Dimethylaminocoumarin-4-acetic acid Chemical Structure

CAS No. : 80883-54-1

Size	Price	Stock	Quantity
250 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

7-Dimethylaminocoumarin-4-acetic acid is a fluorescent probe^[1].

Molecular Weight

247.25

Formula

C₁₃H₁₃NO₄

CAS No.

80883-54-1

Appearance

Solid

Color

Light brown to brown

Emission (Em)

445

Excitation (Ex)

344

SMILES

O=C(O)CC(C1=C(O2)C=C(N(C)C)C=C1)=CC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (404.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0445 mL	20.2224 mL	40.4449 mL
5 mM	0.8089 mL	4.0445 mL	8.0890 mL
10 mM	0.4044 mL	2.0222 mL	4.0445 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (10.11 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.05%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (250 KB)

Handling Instructions (2659 KB)

References

[1]. Portonovo P, et al. First total synthesis of a fluorescent didemnin[J]. Tetrahedron, 2000, 56(23): 3687-3690.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0445 mL	20.2224 mL	40.4449 mL	101.1122 mL
	5 mM	0.8089 mL	4.0445 mL	8.0890 mL	20.2224 mL
	10 mM	0.4044 mL	2.0222 mL	4.0445 mL	10.1112 mL
	15 mM	0.2696 mL	1.3482 mL	2.6963 mL	6.7408 mL
	20 mM	0.2022 mL	1.0111 mL	2.0222 mL	5.0556 mL
	25 mM	0.1618 mL	0.8089 mL	1.6178 mL	4.0445 mL
	30 mM	0.1348 mL	0.6741 mL	1.3482 mL	3.3704 mL
	40 mM	0.1011 mL	0.5056 mL	1.0111 mL	2.5278 mL
	50 mM	0.0809 mL	0.4044 mL	0.8089 mL	2.0222 mL
	60 mM	0.0674 mL	0.3370 mL	0.6741 mL	1.6852 mL
	80 mM	0.0506 mL	0.2528 mL	0.5056 mL	1.2639 mL
	100 mM	0.0404 mL	0.2022 mL	0.4044 mL	1.0111 mL