7-Hydroxycoumarinyl-γ-linolenate 

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A₂ (cPLA₂) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA₂^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
cPLA2α activity assay^[1][2]:
1. Sonicate 200?μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100?high-affinity mixed micelle solution containing 960?μM Triton X-100 (HY-Y1883A), 60?μM DTPC, 50?μM 7-hydroxycoumarinyl-γ-linolenate, 50?mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH?=?7.2), 0.3?mM EDTA (HY-Y0682), 1?mM CaCl₂ and 300?mM KCl.
2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360?nm and emission at 460?nm.
3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

422.56

C₂₇H₃₄O₄

161180-12-7

460

380

CCCCC/C=C\C/C=C\C/C=C\CCCCC(OC1=CC=C(C(O2)=C1)C=CC2=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Xiao Q, et al. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep. 2016 Apr 29;6:25298.  [Content Brief]

  [2]. Huang Z, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5.  [Content Brief]