740 Y-P TFA (Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

Cat. No.: HY-P0175A: FAQs
Data Sheet Handling Instructions

Molarity Calculator

740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone.

For research use only. We do not sell to patients.

740 Y-P TFA Chemical Structure

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of 740 Y-P TFA:

740 Y-P

Other Forms of 740 Y-P TFA:

740 Y-P In-stock

157 Publications Citing Use of MCE 740 Y-P TFA

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 30;39(1):123. [Abstract]; Protein expression levels of p-AktSer473, p-mTORSer2448 with or without 740Y-P treatment are measured by Western blotting. Pretreatment with 740Y-P (PI3K agonist) inhibits downregulation of p-AktSer473 and p-mTORSer2448 expression induced by overexpression of BDH2.

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292. [Abstract]; PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All PI3K Isoform Specific Products:

View All Isoforms

PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

Biological Activity
Purity & Documentation
References
Customer Review

Description

740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone^[1].

In Vitro

740 Y-P TFA (50 μg/ml; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 or wortmannin potently inhibits the mitogenic response stimulated by the 740 Y-P TFA peptide^[1].
740 Y-P TFA (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides^[1].
740 Y-P TFA (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	PC12 cells
Concentration:	30 μM
Incubation Time:	24 hours
Result:	Inhibited the protein expression of LC3-II.

Molecular Weight

3270.70 (free acid)

Formula

C₁₄₁H₂₂₂N₄₃O₃₉PS₃.xC₂HF₃O₂

Sequence

Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{Tyr(PO2)}-Met-Asp-Met-Ser

Sequence Shortening

RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Derossi D, et al. Stimulation of mitogenesis by a cell-permeable PI 3-kinase binding peptide. [Content Brief]

[2]. Xiaoli Feng, et al. Graphene Oxide Induces p62/SQSTM-dependent Apoptosis Through the Impairment of Autophagic Flux and Lysosomal Dysfunction in PC12 Cells. Acta Biomater. 2018 Nov;81:278-292. [Content Brief]

740 Y-P TFA Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

740 Y-P740YPDGFRPDGFR 740Y-PPI3KAutophagyPhosphoinositide 3-kinasethoracicaorticVSMCcancerSH2p85 bindingpeptideInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks