2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 7α-Hydroxy-4-cholesten-3-one

7α-Hydroxy-4-cholesten-3-one is an intermediate in synthesis of bile acids from cholesterol. 7α-Hydroxy-4-cholesten-3-one is a pregnane X receptor (PXR) agonist. 7α-Hydroxy-cholest-4-en-3-one is a biomarker for bile acid loss, irritable bowel syndrome, and other diseases associated with defective bile acid biosynthesis. 7α-Hydroxy-cholest-4-en-3-one is the physiological substrate for CYP8B1.

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7α-Hydroxy-4-cholesten-3-one Chemical Structure

7α-Hydroxy-4-cholesten-3-one Chemical Structure

CAS No. : 3862-25-7

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Based on 1 publication(s) in Google Scholar

Other Forms of 7α-Hydroxy-4-cholesten-3-one:

7α-Hydroxy-4-cholesten-3-one Related Antibodies

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Description

7α-Hydroxy-4-cholesten-3-one is an intermediate in synthesis of bile acids from cholesterol. 7α-Hydroxy-4-cholesten-3-one is a pregnane X receptor (PXR) agonist. 7α-Hydroxy-cholest-4-en-3-one is a biomarker for bile acid loss, irritable bowel syndrome, and other diseases associated with defective bile acid biosynthesis. 7α-Hydroxy-cholest-4-en-3-one is the physiological substrate for CYP8B1[1][2].

IC50 & Target

Endogenous Metabolite[1]
Pregnane X receptor (PXR)[1]
In Vitro

7α-Hydroxy-4-cholesten-3-one is found relatively upstream in the biosynthetic pathway to bile acids (e.g. chenodeoxycholic acid). The first step is the incorporation of the 7α-hydroxy group onto cholesterol by cytochrome P450 7A1, and the second step is the oxidation and isomerization of the 3-hydroxy group and the Δ5,6-double bond by 3β-hydroxy steroid dehydrogenase to yield 7α-Hydroxy-4-cholesten-3-one. The deletion of the gene that expresses P450 27A1, which is found downstream in the bile acid pathway, results in the accumulation of the precursor, 7α-Hydroxy-4-cholesten-3-one[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7α-Hydroxy-4-cholesten-3-one Related Antibodies
In Vivo

7α-Hydroxy-4-cholesten-3-one strongly relates to the hepatic enzymatic activity of CYP7A1 at steady-state conditions as well as during the rapid diurnal changes that occur in the rat. That serum 7α-Hydroxy-4-cholesten-3-one has a pronounced diurnal rhythm[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

400.64

Formula

C27H44O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])[C@H](O)CC4=CC(CC[C@]4(C)[C@@]3([H])CC[C@]12C)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (249.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4960 mL 12.4800 mL 24.9601 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4960 mL 12.4800 mL 24.9601 mL 62.4002 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL 12.4800 mL
10 mM 0.2496 mL 1.2480 mL 2.4960 mL 6.2400 mL
15 mM 0.1664 mL 0.8320 mL 1.6640 mL 4.1600 mL
20 mM 0.1248 mL 0.6240 mL 1.2480 mL 3.1200 mL
25 mM 0.0998 mL 0.4992 mL 0.9984 mL 2.4960 mL
30 mM 0.0832 mL 0.4160 mL 0.8320 mL 2.0800 mL
40 mM 0.0624 mL 0.3120 mL 0.6240 mL 1.5600 mL
50 mM 0.0499 mL 0.2496 mL 0.4992 mL 1.2480 mL
60 mM 0.0416 mL 0.2080 mL 0.4160 mL 1.0400 mL
80 mM 0.0312 mL 0.1560 mL 0.3120 mL 0.7800 mL
100 mM 0.0250 mL 0.1248 mL 0.2496 mL 0.6240 mL
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7α-Hydroxy-4-cholesten-3-one Related Classifications

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Keywords:

7α-Hydroxy-4-cholesten-3-one3862-25-7Endogenous MetaboliteCYP7A17α-hydroxylaseintermediatebileacidscholesterolPXRCYP8B1oxysterolInhibitorinhibitorinhibit

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