2. GPCR/G Protein
  3. Adenosine Receptor
  4. AB-MECA

AB-MECA is a high affinity A3 adenosine receptor agonist with a binding Ki of 430.5 nM for human A3 receptors in CHO cells. AB-MECA can enhance plasma histamine level.

For research use only. We do not sell to patients.

AB-MECA Chemical Structure

AB-MECA Chemical Structure

CAS No. : 152918-26-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 253 In-stock
Solution
10 mM * 1 mL in DMSO USD 253 In-stock
Solid
1 mg USD 72 In-stock
5 mg USD 288 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

AB-MECA Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE AB-MECA

View All Adenosine Receptor Isoform Specific Products:

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Adenosine A1 receptor (A1R) Adenosine A2A receptor (A2AR) Adenosine A2B receptor (A2BR) Adenosine A3 receptor (A3R)

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AB-MECA is a high affinity A3 adenosine receptor agonist with a binding Ki of 430.5 nM for human A3 receptors in CHO cells. AB-MECA can enhance plasma histamine level[1][2][3][4].

IC50 & Target[1][2][3]

Adenosine A3 receptor

 

In Vitro

AB-MECA (1, 10, 100 μM; 24 hours) shows dose-dependent cytotoxicity in human lung cancer cell line A549[2].
[125I]AB-MECA has KD values for binding to A3 receptors in transfected CHO cells and in RBL-2H3 cells are 1.48 and 3.61 nM, respectively[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AB-MECA Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: A549 cell
Concentration: 1, 10, 100 μM
Incubation Time: 24 hours
Result: Shown dose-dependent cytotoxicity.
In Vivo

AB-MECA (3 ug/kg; iv) enhances plasma histamine level in mouse[4].
AB-MECA (0.3 mg/kg; iv) enhances antigen-induced bronchoconstriction in male albino guinea pigs, weighing 180-220 g[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Balb/c mice, weighing 18-25 g[4]
Dosage: 3 ug/kg
Administration: IV; single dose
Result: Induced a 5.9-fold increase in histamine levels in murine plasma.
Molecular Weight

399.40

Formula

C18H21N7O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](N2C=NC3=C(NCC4=CC=C(N)C=C4)N=CN=C23)O[C@H](C(NC)=O)[C@H]1O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 55 mg/mL (137.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5038 mL 12.5188 mL 25.0376 mL
5 mM 0.5008 mL 2.5038 mL 5.0075 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.75 mg/mL (6.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.75 mg/mL (6.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.32%

COA (251 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5038 mL 12.5188 mL 25.0376 mL 62.5939 mL
5 mM 0.5008 mL 2.5038 mL 5.0075 mL 12.5188 mL
10 mM 0.2504 mL 1.2519 mL 2.5038 mL 6.2594 mL
15 mM 0.1669 mL 0.8346 mL 1.6692 mL 4.1729 mL
20 mM 0.1252 mL 0.6259 mL 1.2519 mL 3.1297 mL
25 mM 0.1002 mL 0.5008 mL 1.0015 mL 2.5038 mL
30 mM 0.0835 mL 0.4173 mL 0.8346 mL 2.0865 mL
40 mM 0.0626 mL 0.3130 mL 0.6259 mL 1.5648 mL
50 mM 0.0501 mL 0.2504 mL 0.5008 mL 1.2519 mL
60 mM 0.0417 mL 0.2086 mL 0.4173 mL 1.0432 mL
80 mM 0.0313 mL 0.1565 mL 0.3130 mL 0.7824 mL
100 mM 0.0250 mL 0.1252 mL 0.2504 mL 0.6259 mL
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AB-MECA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AB-MECA152918-26-8Adenosine ReceptorP1 receptorA3 adenosine receptorCHO cellshistamineA549bronchoconstrictionInhibitorinhibitorinhibit

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