AGN 196996

Cat. No.: HY-16682: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AGN 196996 is a potent and selective RARα antagonist with Ki value of 2 nM; little binding affinity for RARβ(Ki=1087 nM) and RARγ(Ki=8523 nM).

For research use only. We do not sell to patients.

AGN 196996 Chemical Structure

CAS No. : 958295-17-5

Size	Price	Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 286	Ask For Quote & Lead Time
Solution
10 mM * 1 mL in DMSO	USD 286	Ask For Quote & Lead Time
Solid
5 mg	USD 270	Ask For Quote & Lead Time
10 mg	USD 405	Ask For Quote & Lead Time
50 mg	USD 1012	Ask For Quote & Lead Time
100 mg	USD 1500	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

3 Publications Citing Use of MCE AGN 196996

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Biological Activity
Purity & Documentation
References
Customer Review

Description

AGN 196996 is a potent and selective RARα antagonist with Ki value of 2 nM; little binding affinity for RARβ(Ki=1087 nM) and RARγ(Ki=8523 nM). IC50 value: 2 nM(Ki) Target: RARα antagonist AGN 196996 shows no activity in transactivation assays, but instead block the gene transcriptional activity induced by ATRA and other RAR agonists.

Molecular Weight

482.32

Formula

C₂₄H₂₀BrNO₅

CAS No.

958295-17-5

Appearance

Solid

Color

White to off-white

SMILES

BrC1=CC(C(NC2=CC=C(C(O)=O)C=C2)=O)=CC(C(C3=CC=C(C)C=C3)=O)=C1OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (518.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0733 mL	10.3666 mL	20.7331 mL
5 mM	0.4147 mL	2.0733 mL	4.1466 mL
10 mM	0.2073 mL	1.0367 mL	2.0733 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.31 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) LCMS (455 KB)

Handling Instructions (2659 KB)

References

[1]. Hammond LA, et al. Antagonists of retinoic acid receptors (RARs) are potent growth inhibitors of prostate carcinoma cells. Br J Cancer. 2001 Aug 3;85(3):453-62. [Content Brief]

[2]. Keedwell RG, et al. An antagonist of retinoic acid receptors more effectively inhibits growth of human prostate cancer cells than normal prostate epithelium. Br J Cancer. 2004 Aug 2;91(3):580-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0733 mL	10.3666 mL	20.7331 mL	51.8328 mL
	5 mM	0.4147 mL	2.0733 mL	4.1466 mL	10.3666 mL
	10 mM	0.2073 mL	1.0367 mL	2.0733 mL	5.1833 mL
	15 mM	0.1382 mL	0.6911 mL	1.3822 mL	3.4555 mL
	20 mM	0.1037 mL	0.5183 mL	1.0367 mL	2.5916 mL
	25 mM	0.0829 mL	0.4147 mL	0.8293 mL	2.0733 mL
	30 mM	0.0691 mL	0.3456 mL	0.6911 mL	1.7278 mL
	40 mM	0.0518 mL	0.2592 mL	0.5183 mL	1.2958 mL
	50 mM	0.0415 mL	0.2073 mL	0.4147 mL	1.0367 mL
	60 mM	0.0346 mL	0.1728 mL	0.3456 mL	0.8639 mL
	80 mM	0.0259 mL	0.1296 mL	0.2592 mL	0.6479 mL
	100 mM	0.0207 mL	0.1037 mL	0.2073 mL	0.5183 mL

AGN 196996 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AGN 196996958295-17-5AGN196996AGN-196996RAR/RXRAutophagyRetinoic acid receptorsRetinoid X receptorsInhibitorinhibitorinhibit

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