ATP-polyamine-biotin

Name: ATP-polyamine-biotin
Brand: MedChemExpress
SKU: HY-D0183
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0183 Purity: 97.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.

For research use only. We do not sell to patients.

ATP-polyamine-biotin Chemical Structure

CAS No. : 1800401-93-7

Size	Price	Stock	Quantity
1 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
2 mg	USD 860	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1680	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 2680	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 5360	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 8550	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE ATP-polyamine-biotin

•Nat Med. 2018 Aug;24(8):1192-1203. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells^[1].

In Vitro

ATP-polyamine-biotin (APB) is incubated with PKA kinase and full-length protein substrate, myelin basic protein (MBP). Biotinylation was observed only in presence of kinase. In addition, MBP biotinylation was lost in the absence of ATP-polyamine-biotin, in presence of the kinase inhibitor staursporine or upon incubation with acid due to cleavage of phosphoramidate bond. Biotinylated kemptide product is observed only in the presence of APB cosubstrate^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

945.85

Formula

C₃₂H₅₈N₁₁O₁₄P₃S

CAS No.

1800401-93-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)NCCCN(C)CCCCN(C)CCCNP(O)(OP(O)(OP(O)(OC[C@@H]3[C@@H](O)[C@@H](O)[C@H](N4C(N=CN=C5N)=C5N=C4)O3)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 6 mg/mL (6.34 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0573 mL	5.2863 mL	10.5725 mL
5 mM	0.2115 mL	1.0573 mL	2.1145 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 7.69 mg/mL (8.13 mM); Clear solution; Need ultrasonic and warming

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (250 KB) HNMR (203 KB) LCMS (327 KB)

Handling Instructions (2659 KB)

References

[1]. Fouda AE, et al. A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation. Angew Chem Int Ed Engl. 2015 Aug 10;54(33):9618-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.0573 mL	5.2863 mL	10.5725 mL	26.4313 mL
H₂O	5 mM	0.2115 mL	1.0573 mL	2.1145 mL	5.2863 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.