S-Adenosyl-L-methionine (Synonyms: S-Adenosyl methionine; Ademetionine; AdoMet)

Cat. No.: HY-B0617: FAQs
Data Sheet SDS COA Handling Instructions

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S-Adenosyl-L-methionine (S-Adenosyl methionine) is an orally active methyl group donor. S-Adenosyl-L-methionine is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine has the potential for, cancer, liver disease and osteoarthritis research.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (S-Adenosyl-L-methionine tosylate and S-Adenosyl-L-methionine disulfate tosylate) that retains the same biological activity.

For research use only. We do not sell to patients.

S-Adenosyl-L-methionine Chemical Structure

CAS No. : 29908-03-0

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Other In-stock Forms of S-Adenosyl-L-methionine:

S-Adenosyl-L-methionine disulfate tosylate

Other Forms of S-Adenosyl-L-methionine:

S-Adenosyl-L-methionine disulfate tosylate In-stock
S-Adenosyl-L-methionine-d₃ Get quote
S-Adenosyl-L-methionine-¹³C Get quote
S-(5′-Adenosyl)-L-methionine Get quote
S-Adenosyl-L-methionine chloride dihydrochloride Get quote
S-Adenosyl-L-methionine (p-toluenesulfonate) Get quote
S-Adenosyl-L-methionine iodide Get quote
S-Adenosyl-L-methionine tosylate Get quote
S-Adenosyl-L-methionine (1,4-butanedisulfonate) Get quote

11 Publications Citing Use of MCE S-Adenosyl-L-methionine

: S-Adenosyl-L-methionine purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2021 Mar 22;95:107545. [Abstract]; S-adenosylmethionine (SAMe) application at 30 min prior to high glucose treatment significantly increased H2S levels compared with the HG group. The cystathionine-β-synthase (CBS) level is obviously elevated in HG + SAMe group compared with the HG group.

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Biological Activity
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References
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Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

S-Adenosyl-L-methionine (300 µM, 24 or 48 h) induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (300 µM, 24 h) decreases the migration of the Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (5-40 μg/mL, 48 h) protects the anticancer effect of 5‑FU by regulating the expression of DNMTs^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[4]

Cell Line:	Cal-33 and JHU-SCC-011 cells
Concentration:	300 µM
Incubation Time:	24 h (Cal-33) or 48 h (HU-SCC-011)
Result:	Showed an approximately 10% and 3%of apoptotic cells respectively.

Cell Cycle Analysis^[4]

Cell Line:	Cal-33 and JHU-SCC-011 cells
Concentration:	300 µM
Incubation Time:	24 h (Cal-33) or 48 h (HU-SCC-011)
Result:	Decreased the expression of cyclin B1, E1 and D1 in the Cal-33 and JHU-SCC-011 cells.

In Vivo

S-Adenosyl-L-methionine (30 mg/kg, p.o., for 3 days) prevents ASD like behaviors induced by early postnatal valproic acid exposure in young mice^[6].
S-Adenosyl-L-methionine (50 and 100 mg/kg, p.o.) shows antiepileptic, memory-enhancing, and antioxidant properties in a Pentylenetetrazole-induced rat epilepsy model^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Valproic acid treated young mice^[6]
Dosage:	30 mg/kg
Administration:	p.o., for 3 days
Result:	Alleviated most ASD like neurobehavioral symptoms. Normalized the redox potential in the prefrontal cortex.

Clinical Trial

Molecular Weight

398.44

Formula

C₁₅H₂₂N₆O₅S

CAS No.

29908-03-0

Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@H]1[C@](O[C@@H]([C@H]1O)C[S+](C)CC[C@H](N)C([O-])=O)([H])N2C3=NC=NC(N)=C3N=C2

Initial Source

Microorganisms

Escherichia coli

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (250.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 43 mg/mL (107.92 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5098 mL	12.5489 mL	25.0979 mL
5 mM	0.5020 mL	2.5098 mL	5.0196 mL
10 mM	0.2510 mL	1.2549 mL	2.5098 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

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Data Sheet (281 KB) SDS (393 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. G M Bressa. S-adenosyl-l-methionine (SAMe) as antidepressant: meta-analysis of clinical studies. Acta Neurol Scand Suppl. 1994;154:7-14. [Content Brief]

[2]. Wadie I Najm, et al. S-adenosyl methionine (SAMe) versus celecoxib for the treatment of osteoarthritis symptoms: a double-blind cross-over trial. [ISRCTN36233495]. BMC Musculoskelet Disord. 2004 Feb 26;5:6. [Content Brief]

[3]. Shelly C Lu, et al. S-adenosylmethionine in liver health, injury, and cancer. Physiol Rev. 2012 Oct;92(4):1515-42. [Content Brief]

[4]. Mosca L, et al. Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224. [Content Brief]

[5]. Ham MS, et al. S-adenosyl methionine specifically protects the anticancer effect of 5-FU via DNMTs expression in human A549 lung cancer cells. Mol Clin Oncol. 2013 Mar;1(2):373-378. [Content Brief]

[6]. Ornoy A, et al. S-adenosyl methionine prevents ASD like behaviors triggered by early postnatal valproic acid exposure in very young mice. Neurotoxicol Teratol. 2019 Jan-Feb;71:64-74. [Content Brief]

[7]. Dhediya RM, et al. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.5098 mL	12.5489 mL	25.0979 mL	62.7447 mL
	5 mM	0.5020 mL	2.5098 mL	5.0196 mL	12.5489 mL
	10 mM	0.2510 mL	1.2549 mL	2.5098 mL	6.2745 mL
	15 mM	0.1673 mL	0.8366 mL	1.6732 mL	4.1830 mL
	20 mM	0.1255 mL	0.6274 mL	1.2549 mL	3.1372 mL
	25 mM	0.1004 mL	0.5020 mL	1.0039 mL	2.5098 mL
	30 mM	0.0837 mL	0.4183 mL	0.8366 mL	2.0915 mL
	40 mM	0.0627 mL	0.3137 mL	0.6274 mL	1.5686 mL
	50 mM	0.0502 mL	0.2510 mL	0.5020 mL	1.2549 mL
	60 mM	0.0418 mL	0.2091 mL	0.4183 mL	1.0457 mL
	80 mM	0.0314 mL	0.1569 mL	0.3137 mL	0.7843 mL
	100 mM	0.0251 mL	0.1255 mL	0.2510 mL	0.6274 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

S-Adenosyl-L-methionine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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