2. Metabolic Enzyme/Protease Apoptosis
  3. Endogenous Metabolite Apoptosis
  4. S-Adenosyl-L-methionine disulfate tosylate

S-Adenosyl-L-methionine disulfate tosylate  (Synonyms: Ademetionine disulfate tosylate; S-Adenosyl methionine disulfate tosylate; AdoMet disulfate tosylate)

Cat. No.: HY-W017770 Purity: 98.53%
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S-Adenosyl-L-methionine disulfate tosylate is the disulfate tosylate form of S-Adenosyl-L-methionine (HY-B0617). S-Adenosyl-L-methionine is an orally active methyl group donor. S-Adenosyl-L-methionine is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine has the potential for, cancer, liver disease and osteoarthritis research.

For research use only. We do not sell to patients.

S-Adenosyl-L-methionine disulfate tosylate Chemical Structure

S-Adenosyl-L-methionine disulfate tosylate Chemical Structure

CAS No. : 97540-22-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 88 In-stock
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Based on 11 publication(s) in Google Scholar

Other Forms of S-Adenosyl-L-methionine disulfate tosylate:

S-Adenosyl-L-methionine disulfate tosylate Related Antibodies

Top Publications Citing Use of Products

    S-Adenosyl-L-methionine disulfate tosylate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2021 Mar 22;95:107545.  [Abstract]

    S-adenosylmethionine (SAMe) application at 30 min prior to high glucose treatment significantly increased H2S levels compared with the HG group. The cystathionine-β-synthase (CBS) level is obviously elevated in HG + SAMe group compared with the HG group.

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    Description

    S-Adenosyl-L-methionine disulfate tosylate is the disulfate tosylate form of S-Adenosyl-L-methionine (HY-B0617). S-Adenosyl-L-methionine is an orally active methyl group donor. S-Adenosyl-L-methionine is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine has the potential for, cancer, liver disease and osteoarthritis research[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    S-Adenosyl-L-methionine (300 µM, 24 or 48 h) induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells[4].
    S-Adenosyl-L-methionine (300 µM, 24 h) decreases the migration of the Cal-33 and JHU-SCC-011 cells[4].
    S-Adenosyl-L-methionine (5-40 μg/mL, 48 h) protects the anticancer effect of 5‑FU by regulating the expression of DNMTs[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    S-Adenosyl-L-methionine disulfate tosylate Related Antibodies

    Apoptosis Analysis[4]

    Cell Line: Cal-33 and JHU-SCC-011 cells
    Concentration: 300 µM
    Incubation Time: 24 h (Cal-33) or 48 h (HU-SCC-011)
    Result: Showed an approximately 10% and 3%of apoptotic cells respectively.

    Cell Cycle Analysis[4]

    Cell Line: Cal-33 and JHU-SCC-011 cells
    Concentration: 300 µM
    Incubation Time: 24 h (Cal-33) or 48 h (HU-SCC-011)
    Result: Decreased the expression of cyclin B1, E1 and D1 in the Cal-33 and JHU-SCC-011 cells.
    In Vivo

    S-Adenosyl-L-methionine (30 mg/kg, p.o., for 3 days) prevents ASD like behaviors induced by early postnatal valproic acid exposure in young mice[6].
    S-Adenosyl-L-methionine (50 and 100 mg/kg, p.o.) shows antiepileptic, memory-enhancing, and antioxidant properties in a Pentylenetetrazole-induced rat epilepsy model[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Valproic acid treated young mice[6]
    Dosage: 30 mg/kg
    Administration: p.o., for 3 days
    Result: Alleviated most ASD like neurobehavioral symptoms.
    Normalized the redox potential in the prefrontal cortex.
    Clinical Trial
    Molecular Weight

    766.80

    Formula

    C22H34N6O16S4
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    N[C@@H](CC[S+](C[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3N)O1)O)O)C)C(O)=O.O=S(O)([O-])=O.O=S(O)(O)=O.OS(=O)(C4=CC=C(C)C=C4)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (65.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 33.33 mg/mL (43.47 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3041 mL 6.5206 mL 13.0412 mL
    5 mM 0.2608 mL 1.3041 mL 2.6082 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (130.41 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.53%

    COA (256 KB) RP-HPLC (206 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.3041 mL 6.5206 mL 13.0412 mL 32.6030 mL
    5 mM 0.2608 mL 1.3041 mL 2.6082 mL 6.5206 mL
    10 mM 0.1304 mL 0.6521 mL 1.3041 mL 3.2603 mL
    15 mM 0.0869 mL 0.4347 mL 0.8694 mL 2.1735 mL
    20 mM 0.0652 mL 0.3260 mL 0.6521 mL 1.6302 mL
    25 mM 0.0522 mL 0.2608 mL 0.5216 mL 1.3041 mL
    30 mM 0.0435 mL 0.2174 mL 0.4347 mL 1.0868 mL
    40 mM 0.0326 mL 0.1630 mL 0.3260 mL 0.8151 mL
    DMSO 50 mM 0.0261 mL 0.1304 mL 0.2608 mL 0.6521 mL
    60 mM 0.0217 mL 0.1087 mL 0.2174 mL 0.5434 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    S-Adenosyl-L-methionine disulfate tosylate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    S-Adenosyl-L-methionine disulfate97540-22-2Ademetionine disulfateS-Adenosyl methionine disulfateAdoMet disulfateEndogenous MetaboliteApoptosisATPmethioninedietarysupplementadenosyltransferasemethyl antidepressantanalgesicosteoarthritisliverdisulfate tosylateInhibitorinhibitorinhibit

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