Atropine sulfate (Synonyms: Tropine tropate sulfate; DL-Hyoscyamine sulfate; Sulfatropinol)

Name: Atropine sulfate
Brand: MedChemExpress
SKU: HY-B1205A
Rating: 5.0 (16 reviews)

Cat. No.: HY-B1205A Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Atropine (Tropine tropate) sulfate is a competitive muscarinic acetylcholine receptor (mAChR) antagonist with IC₅₀ values of 0.39 and 0.71 nM for Human mAChR M₄ and Chicken mAChR M₄, respectively. Atropine sulfate inhibits ACh-induced relaxations in human pulmonary veins. Atropine sulfate can be used for research of anti-myopia and bradycardia.

For research use only. We do not sell to patients.

Atropine sulfate Chemical Structure

CAS No. : 55-48-1

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Based on 16 publication(s) in Google Scholar

Other Forms of Atropine sulfate:

16 Publications Citing Use of MCE Atropine sulfate

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

mAChR4

In Vitro

Atropine (Tropine tropate; 1 μM; pulmonary veins and arteries) sulfate inhibits ACh-induced relaxations in human pulmonary veins^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Atropine (Tropine tropate; 10 mg/kg; i.p.; once, for 40 minutes; Peromyscus sp.) sulfate inhibits the cardiac arrhythmia which normally occurs throughout torpor^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	White-footed mice (Peromyscus sp.)^[2]
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection; once, for 40 minutes
Result:	Increased heart rate was a decrease in cardiac arrhythmia.

Clinical Trial

Molecular Weight

338.41

Formula

C₁₇H₂₄NO₅S_0.5

CAS No.

55-48-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@@H]1C[C@@H](N2C)CC[C@@H]2C1)C(CO)C3=CC=CC=C3.[0.5H2SO4]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (295.50 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (184.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9550 mL	14.7750 mL	29.5500 mL
5 mM	0.5910 mL	2.9550 mL	5.9100 mL
10 mM	0.2955 mL	1.4775 mL	2.9550 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (295.50 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (255 KB) HNMR (272 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. McBrien NA, et, al. How does atropine exert its anti-myopia effects? Ophthalmic Physiol Opt. 2013 May;33(3):373-8. [Content Brief]

[2]. Morhardt JE. Heart rates, breathing rates and the effects of atropine and acetylcholine on white-footed mice (Peromyscus sp.) during daily torpor. Comp Biochem Physiol. 1970 Mar 15;33(2):441-57. [Content Brief]

[3]. Carr BJ, et, al. Myopia-Inhibiting Concentrations of Muscarinic Receptor Antagonists Block Activation of Alpha2A-Adrenoceptors In Vitro. Invest Ophthalmol Vis Sci. 2018 Jun 1;59(7):2778-2791. [Content Brief]

[4]. Walch L, et, al. Evidence for a M(1) muscarinic receptor on the endothelium of human pulmonary veins. Br J Pharmacol. 2000 May;130(1):73-8. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.9550 mL	14.7750 mL	29.5500 mL	73.8749 mL
	5 mM	0.5910 mL	2.9550 mL	5.9100 mL	14.7750 mL
	10 mM	0.2955 mL	1.4775 mL	2.9550 mL	7.3875 mL
	15 mM	0.1970 mL	0.9850 mL	1.9700 mL	4.9250 mL
	20 mM	0.1477 mL	0.7387 mL	1.4775 mL	3.6937 mL
	25 mM	0.1182 mL	0.5910 mL	1.1820 mL	2.9550 mL
	30 mM	0.0985 mL	0.4925 mL	0.9850 mL	2.4625 mL
	40 mM	0.0739 mL	0.3694 mL	0.7387 mL	1.8469 mL
	50 mM	0.0591 mL	0.2955 mL	0.5910 mL	1.4775 mL
	60 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2312 mL
	80 mM	0.0369 mL	0.1847 mL	0.3694 mL	0.9234 mL
	100 mM	0.0295 mL	0.1477 mL	0.2955 mL	0.7387 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Atropine sulfate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atropine55-48-1Tropine tropateDL-Hyoscyamine SulfatropinolmAChRMuscarinic acetylcholine receptoranti-myopiatachyarrhythmiasAchpulmonary veinsbradycardiaInhibitorinhibitorinhibit

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Atropine sulfate (Synonyms: Tropine tropate sulfate; DL-Hyoscyamine sulfate; Sulfatropinol)

Customer Review

Other Forms of Atropine sulfate:

Atropine sulfate Related Antibodies

16 Publications Citing Use of MCE Atropine sulfate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Atropine sulfate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Atropine sulfate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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