2. Anti-infection
  3. Bacterial
  4. Benzalkonium chloride

Benzalkonium chloride  (Synonyms: Alkyldimethylbenzylammonium chloride)

Cat. No.: HY-B2232 Purity: ≥98.0%
COA Handling Instructions

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Benzalkonium chloride is a potent anti-microbial agent, used as a preservative in eye drops.

For research use only. We do not sell to patients.

Benzalkonium chloride Chemical Structure

Benzalkonium chloride Chemical Structure

CAS No. : 8001-54-5

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Solvent
50 mg (510 mg/mL * 98 μL in Water) USD 50 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Benzalkonium chloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Benzalkonium chloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Benzalkonium chloride is a potent anti-microbial agent, used as a preservative in eye drops.

In Vitro

Benzalkonium chloride (0.0001%-0.5%) results in a dose-dependent cytotoxicity in cells. Benzalkonium chloride also shows cytotoxicity against human keratinocytes, with 50% loss of viability of 4 μM. Benzalkonium chloride shows different results in NHEK and NB1RGB cell cultures, and the ED50 of 3.9 and 62 μM, respectively. The clinically used concentration (0.01% Benzalkonium chloride) significantly reduces cell viability, with only 14%-19% of nasal epithelial cells surviving the treatment[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Benzalkonium chloride Related Antibodies
In Vivo

Benzalkonium chloride (0.02%) causes no abnormalities in the nasal epithelium of the monkeys after light and electron microscopical examination. In 4-5 week-old SD rats, 0.01% and 0.1% Benzalkonium chloride induce a time-dependent increase in the thickness of the nasal respiratory epithelium[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

283.88-424.15

Formula

C6H5CH2N(CH3)2RCl (R=C8H17 to C18H37)
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

C[N+](C[(CH2)nCH3])(C)CC1=CC=CC=C1.[Cl-].[n=].[6 -16]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years
Solvent & Solubility
In Vitro: 

Ethanol : 50 mg/mL (Need ultrasonic)

DMSO : 50 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 20 mg/mL

*"≥" means soluble, but saturation unknown.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.25 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (247 KB)

Handling Instructions (2659 KB)
References
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Benzalkonium chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benzalkonium8001-54-5AlkyldimethylbenzylammoniumBacterialInhibitorinhibitorinhibit

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