2. Apoptosis
  3. Caspase
  4. Biotin-VAD-FMK

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

For research use only. We do not sell to patients.

Biotin-VAD-FMK Chemical Structure

Biotin-VAD-FMK Chemical Structure

CAS No. : 1135688-15-1

Size Price Stock Quantity
1 mg USD 290 In-stock
5 mg USD 580 In-stock
10 mg USD 950 In-stock
25 mg USD 1900 In-stock
50 mg USD 3050 In-stock
100 mg USD 4900 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Biotin-VAD-FMK Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

IC50 & Target[1]

Caspase

 

In Vitro

Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Biotin-VAD-FMK Related Antibodies
In Vivo

To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

672.81

Formula

C30H49FN6O8S
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

COC(C[C@@H](C(CF)=O)NC([C@H](C)NC([C@H](C(C)C)NC(CCCCCNC(CCCC[C@@H]1SC[C@H](N2)[C@@H]1NC2=O)=O)=O)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 27.3 mg/mL (40.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4863 mL 7.4315 mL 14.8630 mL
5 mM 0.2973 mL 1.4863 mL 2.9726 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

COA (244 KB) HNMR (286 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4863 mL 7.4315 mL 14.8630 mL 37.1576 mL
5 mM 0.2973 mL 1.4863 mL 2.9726 mL 7.4315 mL
10 mM 0.1486 mL 0.7432 mL 1.4863 mL 3.7158 mL
15 mM 0.0991 mL 0.4954 mL 0.9909 mL 2.4772 mL
20 mM 0.0743 mL 0.3716 mL 0.7432 mL 1.8579 mL
25 mM 0.0595 mL 0.2973 mL 0.5945 mL 1.4863 mL
30 mM 0.0495 mL 0.2477 mL 0.4954 mL 1.2386 mL
40 mM 0.0372 mL 0.1858 mL 0.3716 mL 0.9289 mL
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Biotin-VAD-FMK Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biotin-VAD-FMK1135688-15-1CaspaseInhibitorinhibitorinhibit

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