2. Anti-infection
  3. Bacterial
  4. Cetylpyridinium chloride monohydrate

Cetylpyridinium chloride monohydrate  (Synonyms: Hexadecylpyridinium chloride monohydrate)

Cat. No.: HY-B1289 Purity: 99.90%
SDS COA Handling Instructions

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Cetylpyridinium chloride monohydrate is a cationic quaternary ammonium compound used in some types of mouthwashes, toothpastes, and throat and nasal sprays as an antiseptic that kills bacteria and other microorganisms. Effectively prevents plaque and reduces gingivitis.

For research use only. We do not sell to patients.

Cetylpyridinium chloride monohydrate Chemical Structure

Cetylpyridinium chloride monohydrate Chemical Structure

CAS No. : 6004-24-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 In-stock
Solution
10 mM * 1 mL in DMSO USD 33 In-stock
Solid
500 mg USD 30 In-stock
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5 g   Get quote  

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Based on 2 publication(s) in Google Scholar

Other Forms of Cetylpyridinium chloride monohydrate:

Cetylpyridinium chloride monohydrate Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Cetylpyridinium chloride monohydrate

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cetylpyridinium chloride monohydrate is a cationic quaternary ammonium compound used in some types of mouthwashes, toothpastes, and throat and nasal sprays as an antiseptic that kills bacteria and other microorganisms. Effectively prevents plaque and reduces gingivitis.

Cellular Effect
Cell Line Type Value Description References
Caco-2 IC50
0.62 μM
Compound: CETYLPYRIDINIUM CHLORIDE
Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
10.21203/rs.3.rs-23951/v1
Caco-2 CC50
0.66 μM
Compound: CETYLPYRIDINIUM CHLORIDE
Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
10.21203/rs.3.rs-23951/v1
In Vitro

Cetylpyridinium chloride monohydrate (7.5-50 µg/mL, 5 minutes) disrupts the influenza viral envelope in a dose-dependent manner, leading to structural damage, increased membrane permeability, and nucleoprotein release[2].
Cetylpyridinium chloride monohydrate (10-250 µg/mL, 48 hours) exhibits dose-dependent cytotoxicity in MDCK cells, with a CC50 of 96 µg/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cetylpyridinium chloride monohydrate Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: Madin-Darby Canine Kidney (MDCK) cells
Concentration: 10-250 µg/mL
Incubation Time: 10 minutes at room temperature, followed by 48 hours incubation at 37°C
Result: Led to a decrease in cell viability as the concentration increased. The 50% cytotoxic concentration (CC50) was 96 µg/mL.
In Vivo

Cetylpyridinium chloride monohydrate (5 µL of 0.1% Cetylpyridinium chloride monohydrate, p.o., twice daily for 5 days) enhances survival and reduces influenza-associated morbidity and mortality in C57BL/6 mice infected with mouse-adapted Influenza A (PR8 strain)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice infected with mouse-adapted Influenza A (PR8 strain)[2]
Dosage: 5 µL of ARMS-1 (Cetylpyridinium chloride monohydrate-based formulation, 0.1% Cetylpyridinium chloride monohydrate w/v)
Administration: Oral gavage (p.o.), twice daily for 5 days
Result: Led to higher body weight maintenance in mice and significantly increased survival rates compared to the control group.
Clinical Trial
Molecular Weight

358.00

Formula

C21H40ClNO
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1.O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (279.33 mM)

DMSO : 20 mg/mL (55.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7933 mL 13.9665 mL 27.9330 mL
5 mM 0.5587 mL 2.7933 mL 5.5866 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2 mg/mL (5.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (5.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (279.33 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.90%

SDS (725 KB)

COA (240 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.7933 mL 13.9665 mL 27.9330 mL 69.8324 mL
5 mM 0.5587 mL 2.7933 mL 5.5866 mL 13.9665 mL
10 mM 0.2793 mL 1.3966 mL 2.7933 mL 6.9832 mL
15 mM 0.1862 mL 0.9311 mL 1.8622 mL 4.6555 mL
20 mM 0.1397 mL 0.6983 mL 1.3966 mL 3.4916 mL
25 mM 0.1117 mL 0.5587 mL 1.1173 mL 2.7933 mL
30 mM 0.0931 mL 0.4655 mL 0.9311 mL 2.3277 mL
40 mM 0.0698 mL 0.3492 mL 0.6983 mL 1.7458 mL
50 mM 0.0559 mL 0.2793 mL 0.5587 mL 1.3966 mL
H2O 60 mM 0.0466 mL 0.2328 mL 0.4655 mL 1.1639 mL
80 mM 0.0349 mL 0.1746 mL 0.3492 mL 0.8729 mL
100 mM 0.0279 mL 0.1397 mL 0.2793 mL 0.6983 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cetylpyridinium chloride monohydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cetylpyridinium chloride6004-24-6Hexadecylpyridinium chlorideBacterialInfluenza A (PR8 strain)MDCK cellsAntibioticInhibitorinhibitorinhibit

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