2. Anti-infection
  3. Bacterial
  4. Chloramine-T

Chloramine-T is a titrimetric reagent, and an oxidizing agent. Chloramine-T is an oxidizing biocide.

For research use only. We do not sell to patients.

Chloramine-T Chemical Structure

Chloramine-T Chemical Structure

CAS No. : 127-65-1

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Based on 1 publication(s) in Google Scholar

Chloramine-T Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Chloramine-T is a titrimetric reagent, and an oxidizing agent. Chloramine-T is an oxidizing biocide[1][2].

In Vitro

Chloramine-T direct titration method[2]
1. Dilute the thiol solution (1-5 ml) with distilled water (20-30 ml).
2. Add potassium iodide (~0.5 g) and titrate with standard Chloramine-T solution, using starch (1 ml of 1 % solution), carbon tetrachloride, chloroform, carbon disulfide and benzene (2 ml) as indicators.
3. Add enough concentrated hydrochloric acid to the 2-mercaptoethanol solution to make the acid concentration 0.55 M before titration.

Chloramine-T potentiometric titration method[2]
1. Place a known volume of thiol solution (2-10 ml) in a 100 ml titration cell and dilute the solution with 50 ml of water.
2. Titrate using a potentiometric titration apparatus.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chloramine-T Related Antibodies
Molecular Weight

227.64

Formula

C7H7ClNNaO2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(C)C=C1)(N([Na])Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 130 mg/mL (571.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 41 mg/mL (180.11 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3929 mL 21.9645 mL 43.9290 mL
5 mM 0.8786 mL 4.3929 mL 8.7858 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL (14.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.25 mg/mL (14.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (439.29 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (252 KB) HNMR (267 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.3929 mL 21.9645 mL 43.9290 mL 109.8225 mL
5 mM 0.8786 mL 4.3929 mL 8.7858 mL 21.9645 mL
10 mM 0.4393 mL 2.1965 mL 4.3929 mL 10.9823 mL
15 mM 0.2929 mL 1.4643 mL 2.9286 mL 7.3215 mL
20 mM 0.2196 mL 1.0982 mL 2.1965 mL 5.4911 mL
25 mM 0.1757 mL 0.8786 mL 1.7572 mL 4.3929 mL
30 mM 0.1464 mL 0.7322 mL 1.4643 mL 3.6608 mL
40 mM 0.1098 mL 0.5491 mL 1.0982 mL 2.7456 mL
50 mM 0.0879 mL 0.4393 mL 0.8786 mL 2.1965 mL
60 mM 0.0732 mL 0.3661 mL 0.7322 mL 1.8304 mL
80 mM 0.0549 mL 0.2746 mL 0.5491 mL 1.3728 mL
100 mM 0.0439 mL 0.2196 mL 0.4393 mL 1.0982 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Chloramine-T Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chloramine-T127-65-1BacterialInhibitorinhibitorinhibit

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