2. Cytoskeleton Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related
  3. Microtubule/Tubulin ADC Cytotoxin
  4. MMAF-d8 hydrochloride

MMAF-d8 (hydrochloride)e is a deuterated form of MMAF hydrochloride, which is a microtubule disrupting agent.

For research use only. We do not sell to patients.

MMAF-d<sub>8</sub> hydrochloride Chemical Structure

MMAF-d8 hydrochloride Chemical Structure

Size Price Stock Quantity
1 mg USD 1020 In-stock
5 mg USD 2300 In-stock
10 mg USD 4350 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MMAF-d8 hydrochloride:

MMAF-d8 hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MMAF-d8 hydrochloride

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Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

MMAF-d8 (hydrochloride)e is a deuterated form of MMAF hydrochloride, which is a microtubule disrupting agent.

IC50 & Target

Auristatin

 

In Vitro

MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MMAF-d8 hydrochloride Related Antibodies
In Vivo

The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

776.47

Formula

C39H58D8ClN5O8
Unlabeled CAS

1415246-68-2
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@H](CC1=CC=CC=C1)NC([C@H](C)[C@@H](OC)[C@H]2N(C(C[C@@H](OC)[C@@H](N(C)C([C@@]([2H])(NC([C@@H](NC)C(C)C)=O)C([2H])(C([2H])([2H])[2H])C([2H])([2H])[2H])=O)[C@@H](C)CC)=O)CCC2)=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (128.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2879 mL 6.4394 mL 12.8788 mL
5 mM 0.2576 mL 1.2879 mL 2.5758 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[1]

Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice: When subcutaneous Karpas 299 tumor size reaches 300 mm3, three animals per group receives one injection of 10 mg antibody component/kg body weight of either cAC10-L1-MMAF4 or cBR96-L1-MMAF4 intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5 μm-thin frozen tissue sections are stained using immunohistochemistry evaluation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2879 mL 6.4394 mL 12.8788 mL 32.1970 mL
5 mM 0.2576 mL 1.2879 mL 2.5758 mL 6.4394 mL
10 mM 0.1288 mL 0.6439 mL 1.2879 mL 3.2197 mL
15 mM 0.0859 mL 0.4293 mL 0.8586 mL 2.1465 mL
20 mM 0.0644 mL 0.3220 mL 0.6439 mL 1.6098 mL
25 mM 0.0515 mL 0.2576 mL 0.5152 mL 1.2879 mL
30 mM 0.0429 mL 0.2146 mL 0.4293 mL 1.0732 mL
40 mM 0.0322 mL 0.1610 mL 0.3220 mL 0.8049 mL
50 mM 0.0258 mL 0.1288 mL 0.2576 mL 0.6439 mL
60 mM 0.0215 mL 0.1073 mL 0.2146 mL 0.5366 mL
80 mM 0.0161 mL 0.0805 mL 0.1610 mL 0.4025 mL
100 mM 0.0129 mL 0.0644 mL 0.1288 mL 0.3220 mL
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MMAF-d8 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMAF-d8Microtubule/TubulinADC CytotoxinADC PayloadInhibitorinhibitorinhibit

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