2. Membrane Transporter/Ion Channel Cell Cycle/DNA Damage
  3. VDAC RAD51
  4. DIDS sodium salt

DIDS sodium salt  (Synonyms: MDL101114ZA)

Cat. No.: HY-D0086 Purity: 99.13%
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DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research.

For research use only. We do not sell to patients.

DIDS sodium salt Chemical Structure

DIDS sodium salt Chemical Structure

CAS No. : 67483-13-0

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5 mg USD 29 In-stock
10 mg USD 40 In-stock
50 mg USD 80 In-stock
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Based on 13 publication(s) in Google Scholar

Other Forms of DIDS sodium salt:

DIDS sodium salt Related Antibodies

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  • Protocol

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Description

DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research[1][2][3][4].

IC50 & Target

ABCA1[1], VDAC1[2].
In Vitro

DIDS sodium salt (0-10 μM) inhibits RAD51-mediated strand exchange[1].
DIDS sodium salt (0-20 μM) inhibits DNA binding by RAD51[1].
DIDS sodium salt (10 μM; 0-60 min) stimulates the ATP hydrolyzing activity of RAD51 in the absence of DNA[1].
DIDS sodium salt (50-400 μM) prevents effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect, and dose at 400 μM insifnificantly decreases the cell viability[2].
DIDS sodium salt (100 μM) clearly inhibits caspase-3 and caspase-9 activation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DIDS sodium salt Related Antibodies
Molecular Weight

498.48

Formula

C16H8N2Na2O6S4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC(N=C=S)=CC=C1/C=C/C2=CC=C(N=C=S)C=C2S(=O)(O[Na])=O)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (250.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 33.33 mg/mL (66.86 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0061 mL 10.0305 mL 20.0610 mL
5 mM 0.4012 mL 2.0061 mL 4.0122 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.13%

SDS (585 KB)

COA (252 KB) HNMR (271 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References
Cell Assay

Cells (5×105 cells/mL) are seeded in the 35-mm Petri dishes and cultured for 72 h at 37°C. Cells are pre-incubated with ALA, and then DIDS of diverse concentrations (0-200 μM) is added before exposed to ultrasound. Cell apoptosis is detected. Briefly, seeded cells (5×105 cells/mL) are pre-treated with test compounds at the indicated concentrations for 1 h (DIDS and NAC), 1.5 h (BAPTA) or with siRNA and exposed to ultrasound. Six hours after ALA-SDT treatment, cells are collected[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0061 mL 10.0305 mL 20.0610 mL 50.1525 mL
5 mM 0.4012 mL 2.0061 mL 4.0122 mL 10.0305 mL
10 mM 0.2006 mL 1.0030 mL 2.0061 mL 5.0152 mL
15 mM 0.1337 mL 0.6687 mL 1.3374 mL 3.3435 mL
20 mM 0.1003 mL 0.5015 mL 1.0030 mL 2.5076 mL
25 mM 0.0802 mL 0.4012 mL 0.8024 mL 2.0061 mL
30 mM 0.0669 mL 0.3343 mL 0.6687 mL 1.6717 mL
40 mM 0.0502 mL 0.2508 mL 0.5015 mL 1.2538 mL
50 mM 0.0401 mL 0.2006 mL 0.4012 mL 1.0030 mL
60 mM 0.0334 mL 0.1672 mL 0.3343 mL 0.8359 mL
DMSO 80 mM 0.0251 mL 0.1254 mL 0.2508 mL 0.6269 mL
100 mM 0.0201 mL 0.1003 mL 0.2006 mL 0.5015 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DIDS sodium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DIDS67483-13-0MDL101114ZAVDACRAD51Voltage-dependent anion channelhomologous pairinganion exchangered blood cell membraneInhibitorinhibitorinhibit

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