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  4. Docosahexaenoic acid methyl ester

Docosahexaenoic acid methyl ester  (Synonyms: Methyl docosahexaenoate; all cis-DHA methyl ester)

Cat. No.: HY-101541 Purity: ≥98.0%
SDS COA Handling Instructions

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Docosahexaenoic Acid methyl ester is a methylated docosahexaenoic acid analog which can be intercalated into membrane phospholipids without being oxidized or hydrolyzed.

For research use only. We do not sell to patients.

Docosahexaenoic acid methyl ester Chemical Structure

Docosahexaenoic acid methyl ester Chemical Structure

CAS No. : 2566-90-7

Size Price Stock Quantity
Solution
10 mM * 1 mL in Ethanol USD 41 In-stock
Liquid
50 mg USD 38 In-stock
100 mg USD 60 In-stock
500 mg USD 230 In-stock
1 g USD 370 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Docosahexaenoic acid methyl ester:

Docosahexaenoic acid methyl ester Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Docosahexaenoic Acid methyl ester is a methylated docosahexaenoic acid analog which can be intercalated into membrane phospholipids without being oxidized or hydrolyzed.

In Vitro

Sharp wave (SPW) incidence relative to baseline appears to decrease following Docosahexaenoic Acid methyl ester (DHA-Me) application[1]. There is no generation of a new protein band when bovine serum albumin (BSA) is exposed to the Fe2+ and ascorbic acid (AsA) mixed-function oxidation system in the absence of Docosahexaenoic Acid methyl ester (DHA). However, the high-molecular-weight protein band is observed after only 24 h when BSA is incubated with DHA. Incubation of BSA with 1.0 mM DHA leads to a substantial increase in protein carbonyl content and the addition of oxygen radical scavengers leads to a substantial decrease in protein carbonyl content[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Docosahexaenoic acid methyl ester Related Antibodies
Molecular Weight

342.51

Formula

C23H34O2
CAS No.
Appearance

Liquid (Density: 0.921 g/cm3)

Color

Colorless to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCC(OC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (291.96 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9196 mL 14.5981 mL 29.1962 mL
5 mM 0.5839 mL 2.9196 mL 5.8392 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (237 KB) HNMR (230 KB) LCMS (374 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[2]

Protein (1 mg/mL) is incubated with Docosahexaenoic Acid methyl ester (DHA) (1.0 mM) in 50 mM HEPES buffer (pH 7.4) containing 0.2% (w/v) of Tween 20 at 37°C. Mannitol, histidine, sodium benzoate, or KI is added at final concentration of 0.5 M, 0.2 M, 0.5 M, or 0.1 M, respectively. Reactive oxygen species are generated by addition of FeSO4 (1 μM) and ascorbic acid (AsA, 20 μM)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.9196 mL 14.5981 mL 29.1962 mL 72.9906 mL
5 mM 0.5839 mL 2.9196 mL 5.8392 mL 14.5981 mL
10 mM 0.2920 mL 1.4598 mL 2.9196 mL 7.2991 mL
15 mM 0.1946 mL 0.9732 mL 1.9464 mL 4.8660 mL
20 mM 0.1460 mL 0.7299 mL 1.4598 mL 3.6495 mL
25 mM 0.1168 mL 0.5839 mL 1.1678 mL 2.9196 mL
30 mM 0.0973 mL 0.4866 mL 0.9732 mL 2.4330 mL
40 mM 0.0730 mL 0.3650 mL 0.7299 mL 1.8248 mL
50 mM 0.0584 mL 0.2920 mL 0.5839 mL 1.4598 mL
60 mM 0.0487 mL 0.2433 mL 0.4866 mL 1.2165 mL
80 mM 0.0365 mL 0.1825 mL 0.3650 mL 0.9124 mL
100 mM 0.0292 mL 0.1460 mL 0.2920 mL 0.7299 mL
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Docosahexaenoic acid methyl ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Docosahexaenoic acid methyl ester2566-90-7Methyl docosahexaenoate all cis-DHA methyl esterOthersInhibitorinhibitorinhibit

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