2. Anti-infection
  3. Bacterial Antibiotic
  4. Ethionamide

Ethionamide  (Synonyms: 2-Ethylthioisonicotinamide)

Cat. No.: HY-B0276 Purity: 99.73%
SDS COA Handling Instructions Technical Support

Ethionamide (2-ethylthioisonicotinamide) is a second-line anti-tuberculosis antibiotic with antibacterial effect and oral activity.

For research use only. We do not sell to patients.

Ethionamide Chemical Structure

Ethionamide Chemical Structure

CAS No. : 536-33-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ethionamide:

Ethionamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ethionamide

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ethionamide (2-ethylthioisonicotinamide) is a second-line anti-tuberculosis antibiotic with antibacterial effect and oral activity[1][2][3][4].

In Vitro

Ethionamide strongly inhibits mycolic acid synthesis in BCG strain Mycobacterium bovis cells. For M. tuberculosis Canetti, Mycobacterium sp. strain CR21, and ATCC 23366, the MIC values are 0.5, 3, and 60 μg/ml, respectively[2].
Ethionamide (60 μg/ml, 0-120 min) concurrently increases unsaturated mycolic acids and decreases oxygenated mycolic acids in M. aurum ATCC 23366[2].
Ethionamide's S-oxidation is primarily catalyzed by FMOs, particularly in the liver and lung[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ethionamide Related Antibodies

Cell Proliferation Assay[2]

Cell Line: M.aurum ATCC 23366
Concentration: 60 μg/ml
Incubation Time: 0, 20, 40, 60, 80, 100, 120 min
Result: Unsaturated mycolic acid increases, oxygenated mycolic acid decreases.
In Vivo


Pharmacokinetic parameters of ethionamide in mice[4]

Sex Tmax (h) Cmax (μg/mL)
Male 1 15.1±34.4
Femal 1±0.5 15.1±14.8

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

166.25

Formula

C8H10N2S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

S=C(C1=CC(CC)=NC=C1)N
Structure Classification
Initial Source

Mycobacterium tuberculosis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (601.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.0151 mL 30.0754 mL 60.1507 mL
5 mM 1.2030 mL 6.0151 mL 12.0301 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (30.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (30.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.73%

SDS (557 KB)

COA (255 KB) HNMR (119 KB) RP-HPLC (225 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.0151 mL 30.0754 mL 60.1507 mL 150.3769 mL
5 mM 1.2030 mL 6.0151 mL 12.0301 mL 30.0754 mL
10 mM 0.6015 mL 3.0075 mL 6.0151 mL 15.0377 mL
15 mM 0.4010 mL 2.0050 mL 4.0100 mL 10.0251 mL
20 mM 0.3008 mL 1.5038 mL 3.0075 mL 7.5188 mL
25 mM 0.2406 mL 1.2030 mL 2.4060 mL 6.0151 mL
30 mM 0.2005 mL 1.0025 mL 2.0050 mL 5.0126 mL
40 mM 0.1504 mL 0.7519 mL 1.5038 mL 3.7594 mL
50 mM 0.1203 mL 0.6015 mL 1.2030 mL 3.0075 mL
60 mM 0.1003 mL 0.5013 mL 1.0025 mL 2.5063 mL
80 mM 0.0752 mL 0.3759 mL 0.7519 mL 1.8797 mL
100 mM 0.0602 mL 0.3008 mL 0.6015 mL 1.5038 mL
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Ethionamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethionamide536-33-42-EthylthioisonicotinamideBacterialAntibioticAnti-tuberculosis antibioticsantibacterialInhibitorinhibitorinhibit

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