FGFR4-IN-1

Name: FGFR4-IN-1
Brand: MedChemExpress
SKU: HY-100631
Rating: 5.0 (1 reviews)

Cat. No.: HY-100631 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

FGFR4-IN-1 is a potent inhibiotr of FGFR4 with IC₅₀ of 0.7 nM.

For research use only. We do not sell to patients.

FGFR4-IN-1 Chemical Structure

CAS No. : 1708971-72-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 130	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 130	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 57	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE FGFR4-IN-1

•Heliyon. 2024 May 13.

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FGFR FGFR1 FGFR2 FGFR3 FGFR4 FGF21

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

FGFR4-IN-1 is a potent inhibiotr of FGFR4 with IC₅₀ of 0.7 nM.

IC₅₀ & Target^[1]

FGFR4

0.7 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Hep 3B2	IC₅₀	8.9 nM Compound: 6a	Antiproliferative activity against human Hep3B cells with high FGFR4 expression assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method Antiproliferative activity against human Hep3B cells with high FGFR4 expression assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method	[PMID: 30987781]
Huh-7	IC₅₀	17.3 nM Compound: 6a	Antiproliferative activity against human HuH7 cells with high FGFR4 expression assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method Antiproliferative activity against human HuH7 cells with high FGFR4 expression assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method	[PMID: 30987781]
Huh-7	IC₅₀	25 nM Compound: 69	Antiproliferative activity against human HuH7 cells assessed as reduction in cell viability incubated for 3 days by cell proliferation assay Antiproliferative activity against human HuH7 cells assessed as reduction in cell viability incubated for 3 days by cell proliferation assay	[PMID: 32930584]
SK-HEP1	IC₅₀	> 10000 nM Compound: 6a	Antiproliferative activity against human SKHEP1 cells without FGFR4 gene amplification assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method Antiproliferative activity against human SKHEP1 cells without FGFR4 gene amplification assessed as inhibition of cell proliferation incubated for 72 hrs by CellTiter-Glo method	[PMID: 30987781]

In Vitro

FGFR4-IN-1 significantly inhibits the proliferation of HuH-7 hepatocellular carcinoma cells with IC₅₀ of 7.8 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

493.52

Formula

C₂₄H₂₇N₇O₅

CAS No.

1708971-72-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCCC2=C1N=C(C=O)C(CN3C(COCC3)=O)=C2)NC4=NC=C(C#N)C(NCCOC)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 6.4 mg/mL (12.97 mM; Need warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0263 mL	10.1313 mL	20.2626 mL
5 mM	0.4053 mL	2.0263 mL	4.0525 mL
10 mM	0.2026 mL	1.0131 mL	2.0263 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (251 KB) RP-HPLC (261 KB)

Handling Instructions (2659 KB)

References

[1]. Ring-fused bicyclic pyridyl derivatives as fgfr4 inhibitors. WO 2015059668 A1?

Cell Assay
^[1]

Methylene blue staining proliferation assay (MBS): The effect of compounds on cell proliferation is assessed using HuH-7 hepatocellular carcinoma cells are cultured in the vendor-recommended medium. Specifically, 5000 cells/well are seeded in 96-well tissue culture plates in a total media volume of 100 μL/well and increasing compound dilutions or DMSO are added 24 hours thereafter in triplicates. 72 hours after compound addition, cells are fixed by adding 25 and incubated for 10 minutes at room temperature. Cells are washed three times with H₂O. Cells are washed 3 times with H₂O, 200 mL/well and then lysed by adding 200 mL/well of 3% HCl for 30 minutes at room temperature with shaking. Optical density is measured at A650 nm. The concentration of compound providing 50% of proliferation inhibition with respect to DMSO-treated cells is determined (IC₅₀) using XLFit software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Ring-fused bicyclic pyridyl derivatives as fgfr4 inhibitors. WO 2015059668 A1?

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0263 mL	10.1313 mL	20.2626 mL	50.6565 mL
	5 mM	0.4053 mL	2.0263 mL	4.0525 mL	10.1313 mL
	10 mM	0.2026 mL	1.0131 mL	2.0263 mL	5.0657 mL

FGFR4-IN-1 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FGFR4-IN-11708971-72-5FGFRFibroblast growth factor receptorInhibitorinhibitorinhibit

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FGFR4-IN-1

Customer Review

FGFR4-IN-1 Related Antibodies

1 Publications Citing Use of MCE FGFR4-IN-1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All FGFR Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

FGFR4-IN-1 Related Antibodies

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Complete Stock Solution Preparation Table

FGFR4-IN-1 Related Classifications

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