INH1 (Synonyms: IBT13131)

Name: INH1
Brand: MedChemExpress
SKU: HY-16660
Rating: 4.5 (1 reviews)

Cat. No.: HY-16660 Purity: 99.85%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

INH1 specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding. INH1 shows promising cancer inhibition activity both in vitro and in vivo.

For research use only. We do not sell to patients.

INH1 Chemical Structure

CAS No. : 313553-47-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
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Customer Review

Based on 1 publication(s) in Google Scholar

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References
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Description

INH1 specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding. INH1 shows promising cancer inhibition activity both in vitro and in vivo^[1].

IC₅₀ & Target

NEK2

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	8.8 μM Compound: INH1	Cytotoxicity against human HeLa cells after 4 days by XTT assay Cytotoxicity against human HeLa cells after 4 days by XTT assay	[PMID: 19243176]
K562	IC₅₀	11.7 μM Compound: INH1	Cytotoxicity against human K562 cells after 4 days by XTT assay Cytotoxicity against human K562 cells after 4 days by XTT assay	[PMID: 19243176]
MDA-MB-231	IC₅₀	0.839 μM Compound: 3o	Inhibition of cell migration in human MDA-MB-231 cells assessed as total number of cells after 24 hrs by transwell migration assay Inhibition of cell migration in human MDA-MB-231 cells assessed as total number of cells after 24 hrs by transwell migration assay	[PMID: 23526571]
MDA-MB-231	IC₅₀	8.6 μM Compound: INH1	Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay	[PMID: 19243176]
MDA-MB-468	IC₅₀	10.5 μM Compound: INH1	Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay	[PMID: 19243176]

In Vitro

INH1 (25 μM, 24 h) treatment resulted in reduced association of Hec1 with kinetochore and decrease of global Nek2 protein level^[1].
INH1 exhibits GI₅₀ values of 10.5 μM (in MDA-MB-468 cells), 15 μM (in SKBR3 cells), 10.5 μM (in T47D cells), 20.5 μM (in MDA-MB-361 cells), 15 μM (in ZR-75-1 cells), 15 μM (in HBL 100 cells), 15.5 μM (in MDA-MB-435 cells), 11 μM (in HS578T cells) and 41 μM (in MCF10A cells), respectively^[1].
INH1 (5k) has an IC₅₀ value of 176 nM in the dose-dependent transwell migration assays in MDA-MB-231 cells. INH1 (5k) substantially reduces cellular f-actin and prevented localization of fascin to actin-rich membrane protrusions^[2].
INH1 induces abnormal mitotic processes, as well as cell apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	MCF10A cells.
Concentration:	10 μM.
Incubation Time:	12 days.
Result:	Effectively inhibits the proliferation of human breast cancer lines.

Western Blot Analysis^[1]

Cell Line:	MCF10A cells.
Concentration:	25 μM.
Incubation Time:	24 h.
Result:	Nek2 reduction in INH1-treated cells may be independent of Hec1.

In Vivo

INH1 (50 or 100 mg/kg, i.p., every other day/25 cycles) inhibits tumor outgrowth in a xenografted breast cancer model in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenografted nude mice breast cancer model^[1].
Dosage:	50 or 100 mg/kg.
Administration:	I.P., every other day/25 cycles.
Result:	Inhibited tumor growth.

Molecular Weight

308.40

Formula

C₁₈H₁₆N₂OS

CAS No.

313553-47-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=NC(C2=CC=C(C)C=C2C)=CS1)C3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (324.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2425 mL	16.2127 mL	32.4254 mL
5 mM	0.6485 mL	3.2425 mL	6.4851 mL
10 mM	0.3243 mL	1.6213 mL	3.2425 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 17% Solutol HS-15 in Saline
Solubility: 20 mg/mL (64.85 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (249 KB) HNMR (264 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Wu G, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008 Oct 15;68(20):8393-9. [Content Brief]

[2]. Shilong Zheng, et al. Discovery of a Series of Thiazole Derivatives as Novel Inhibitors of Metastatic Cancer Cell Migration and Invasion. ACS Med Chem Lett. 2013 Feb 14; 4(2): 191–196. [Content Brief]

[3]. Yongxia Zhu, et al. Small Molecule TH-39 Potentially Targets Hec1/Nek2 Interaction and Exhibits Antitumor Efficacy in K562 Cells via G0/G1 Cell Cycle Arrest and Apoptosis Induction. Cell Physiol Biochem. 2016;40(1-2):297-308. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2425 mL	16.2127 mL	32.4254 mL	81.0636 mL
	5 mM	0.6485 mL	3.2425 mL	6.4851 mL	16.2127 mL
	10 mM	0.3243 mL	1.6213 mL	3.2425 mL	8.1064 mL
	15 mM	0.2162 mL	1.0808 mL	2.1617 mL	5.4042 mL
	20 mM	0.1621 mL	0.8106 mL	1.6213 mL	4.0532 mL
	25 mM	0.1297 mL	0.6485 mL	1.2970 mL	3.2425 mL
	30 mM	0.1081 mL	0.5404 mL	1.0808 mL	2.7021 mL
	40 mM	0.0811 mL	0.4053 mL	0.8106 mL	2.0266 mL
	50 mM	0.0649 mL	0.3243 mL	0.6485 mL	1.6213 mL
	60 mM	0.0540 mL	0.2702 mL	0.5404 mL	1.3511 mL
	80 mM	0.0405 mL	0.2027 mL	0.4053 mL	1.0133 mL
	100 mM	0.0324 mL	0.1621 mL	0.3243 mL	0.8106 mL

INH1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

INH1313553-47-8IBT13131INH 1INH-1IBT 13131IBT-13131NEKsApoptosisNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed KinasesInhibitorinhibitorinhibit

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INH1 (Synonyms: IBT13131)

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INH1 Related Antibodies

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•Related Small Molecules:

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View All NEKs Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

INH1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

INH1 Related Classifications

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