IPSU

Name: IPSU
Brand: MedChemExpress
SKU: HY-13796
Rating: 4.5 (1 reviews)

Cat. No.: HY-13796 Purity: 98.10%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IPSU is a selective, orally available and brain penetrant OX2R antagonist with a pK_i of 7.85.

For research use only. We do not sell to patients.

IPSU Chemical Structure

CAS No. : 1373765-19-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 149	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 149	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 216	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE IPSU

•Molecules. 2020 Feb 25;25(5):1018. [Abstract]

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OX₂ Receptor OX₁ Receptor

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IPSU is a selective, orally available and brain penetrant OX2R antagonist with a pK_i of 7.85.

IC₅₀ & Target

pKi: 7.85 (OX2R), 6.29 (OX1R)^[1]

In Vitro

Orexin receptor antagonists represent attractive targets for the development of drugs for the treatment of insomnia. IPSU binds rapidly and reaches equilibrium very quickly in binding and/or functional assays^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

IPSU has low blood clearance, shows high maximal blood exposure and AUC after oral dosing. It exhibits an acceptable absolute oral bioavailability and a brain/blood concentration ratio that indicated favorable brain penetration. IPSU increases sleep when dosed during the mouse active phase (lights off); IPSU induces sleep primarily by increasing NREM sleep. IPSU shows a fast onset of action, with a clear increase in total sleep time during the first hour afterdosing. The effect lasts 4-5 h, after which time the total sleep time per hour is the same as on vehicle day ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

405.49

Formula

C₂₃H₂₇N₅O₂

CAS No.

1373765-19-5

Appearance

Solid

Color

White to off-white

SMILES

COC1=CC=NC(N(CC2)CCC2(C3=O)CCCN3CC4=CNC5=CC=CC=C54)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 30 mg/mL (73.98 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4662 mL	12.3308 mL	24.6615 mL
5 mM	0.4932 mL	2.4662 mL	4.9323 mL
10 mM	0.2466 mL	1.2331 mL	2.4662 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (247 KB) LCMS (121 KB)

Handling Instructions (2659 KB)

References

[1]. Betschart C, et al. Identification of a novel series of orexin receptor antagonists with a distinct effect on sleeparchitecture for the treatment of insomnia. J Med Chem. 2013 Oct 10;56(19):7590-607. [Content Brief]

[2]. Callander GE, et al. Kinetic properties of "dual" orexin receptor antagonists at OX1R and OX2R orexin receptors. Front Neurosci. 2013 Dec 3;7:230. [Content Brief]

[3]. Hoyer D, et al. Distinct effects of IPSU and suvorexant on mouse sleep architecture. [Content Brief]

Kinase Assay ^[2]	Competition experiments are performed with a single concentration of radioligand and six concentrations of competitor (unlabeled ligands; BBAC, almorexant, SB-649868, suvorexant, filorexant or IPSU). 4.6 nM [³H]-BBAC is added simultaneously with various concentrations of unlabeled ligand (0.1 nM-10 μM) to membranes (150 μL/well) in 50 μL/well of assay buffer with a total volume of 250 μL/well. The amount of [³H]-BBAC bound to receptors is determined at room temperature at different time points (ranging from 15 min to 4 h) and terminated by rapid vacuum filtration and liquid scintillation counting^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice: Freely moving C57Bl/6 mice with chronically implanted electrodes are well abituated to the experiment boxes and had access to food and ater ad libitum. The test compounds (IPSU) or vehicle are administered per os as a suspension in 0.5% methylcellulose immediately prior to lights off and start of recording. Movement is recorded using infrared sensors in the roof of the box. EEG/EMG signals and motility data are used to score 10 s epochs into wake, NREM sleep, and REM sleep. Each animal served as its own control by application and recording of vehicle the day before compound (IPSU) dosing^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Betschart C, et al. Identification of a novel series of orexin receptor antagonists with a distinct effect on sleeparchitecture for the treatment of insomnia. J Med Chem. 2013 Oct 10;56(19):7590-607. [Content Brief] [2]. Callander GE, et al. Kinetic properties of "dual" orexin receptor antagonists at OX1R and OX2R orexin receptors. Front Neurosci. 2013 Dec 3;7:230. [Content Brief] [3]. Hoyer D, et al. Distinct effects of IPSU and suvorexant on mouse sleep architecture. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4662 mL	12.3308 mL	24.6615 mL	61.6538 mL
	5 mM	0.4932 mL	2.4662 mL	4.9323 mL	12.3308 mL
	10 mM	0.2466 mL	1.2331 mL	2.4662 mL	6.1654 mL
	15 mM	0.1644 mL	0.8221 mL	1.6441 mL	4.1103 mL
	20 mM	0.1233 mL	0.6165 mL	1.2331 mL	3.0827 mL
	25 mM	0.0986 mL	0.4932 mL	0.9865 mL	2.4662 mL
	30 mM	0.0822 mL	0.4110 mL	0.8221 mL	2.0551 mL
	40 mM	0.0617 mL	0.3083 mL	0.6165 mL	1.5413 mL
	50 mM	0.0493 mL	0.2466 mL	0.4932 mL	1.2331 mL
	60 mM	0.0411 mL	0.2055 mL	0.4110 mL	1.0276 mL

IPSU Related Classifications

Neurological Disease Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IPSU1373765-19-5Orexin Receptor (OX Receptor)Hypocretin ReceptorHCRT ReceptorInhibitorinhibitorinhibit

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IPSU

Customer Review

IPSU Related Antibodies

1 Publications Citing Use of MCE IPSU

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•Related Screening Libraries:

•Related Small Molecules:

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View All Orexin Receptor (OX Receptor) Isoform Specific Products:

Biological Activity

Protocol

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References

Customer Review

IPSU Related Antibodies

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Complete Stock Solution Preparation Table

IPSU Related Classifications

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