ISO-1 (Synonyms: MIF Antagonist)

Name: ISO-1
Brand: MedChemExpress
SKU: HY-16692
Rating: 5.0 (15 reviews)

Cat. No.: HY-16692 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

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ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC₅₀ of 7 μM.

For research use only. We do not sell to patients.

ISO-1 Chemical Structure

CAS No. : 478336-92-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 88	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 88	In-stock	Estimated Time of Arrival: December 31
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5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 620	In-stock	Estimated Time of Arrival: December 31
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Based on 15 publication(s) in Google Scholar

13 Publications Citing Use of MCE ISO-1

: ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]; ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.

: ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]; ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.

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Description

ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC₅₀ of 7 μM.

IC₅₀ & Target

IC50: 7 μM (MIF)^[1]

In Vitro

ISO-1 (0.1-20 μM; 16 hours) has a slight inhibitory effect on Cox-2 secretion without the addition of recombinant MIF^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	RAW 264.7 macrophage cells
Concentration:	0.1μM，1μM，10μM，20μM
Incubation Time:	16 hours
Result:	Suppressed Cox-2 secretion.

In Vivo

ISO-1 (injected intraperitoneally; 3.5-35 mg/kg; twice daily; 2 weeks) improves the survival rate from lethal endotoxemia and shows the anti-inflammatory effect^[2].
ISO-1 (intraperitoneal injection; 35 mg/kg; twice daily; 3 days) causes a significant reduction in average implant size and decreases Flk1 expression in implants^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6 MIF^+/+ and MIF^–/– mice^[2]
Dosage:	3.5-35 mg/kg
Administration:	Injected intraperitoneally; 3.5-35 mg/kg; twice a day ; 2 weeks
Result:	Was protective against lethal sepsis.

Animal Model:	Female C57BL/6-Tg(ACTB-EGFP)1Osb/J mice^[3]
Dosage:	35 mg/kg
Administration:	Intraperitoneal injection; 35 mg/kg; twice daily; 3 days
Result:	Reduced average endometriotic implant size.

Molecular Weight

235.24

Formula

C₁₂H₁₃NO₄

CAS No.

478336-92-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)CC1ON=C(C(C=C2)=CC=C2O)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (425.10 mM; Need ultrasonic)

DMSO : 50 mg/mL (212.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2510 mL	21.2549 mL	42.5098 mL
5 mM	0.8502 mL	4.2510 mL	8.5020 mL
10 mM	0.4251 mL	2.1255 mL	4.2510 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 10 mg/mL (42.51 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (252 KB) HNMR (420 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Lubetsky JB, et al. The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J Biol Chem. 2002 Jul 12;277(28):24976-82. [Content Brief]

[2]. Al-Abed Y, et al. ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J Biol Chem. 2005 Nov 4;280(44):36541-4. [Content Brief]

[3]. Nothnick WB, et al. Inhibition of macrophage migration inhibitory factor reduces endometriotic implant size in mice with experimentally induced disease. J Endometr. 2011 Sep 30;3(3):135-142. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	4.2510 mL	21.2549 mL	42.5098 mL	106.2744 mL
	5 mM	0.8502 mL	4.2510 mL	8.5020 mL	21.2549 mL
	10 mM	0.4251 mL	2.1255 mL	4.2510 mL	10.6274 mL
	15 mM	0.2834 mL	1.4170 mL	2.8340 mL	7.0850 mL
	20 mM	0.2125 mL	1.0627 mL	2.1255 mL	5.3137 mL
	25 mM	0.1700 mL	0.8502 mL	1.7004 mL	4.2510 mL
	30 mM	0.1417 mL	0.7085 mL	1.4170 mL	3.5425 mL
	40 mM	0.1063 mL	0.5314 mL	1.0627 mL	2.6569 mL
	50 mM	0.0850 mL	0.4251 mL	0.8502 mL	2.1255 mL
	60 mM	0.0708 mL	0.3542 mL	0.7085 mL	1.7712 mL
	80 mM	0.0531 mL	0.2657 mL	0.5314 mL	1.3284 mL
	100 mM	0.0425 mL	0.2125 mL	0.4251 mL	1.0627 mL

ISO-1 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ISO-1478336-92-4MIF AntagonistISO1ISO 1Macrophage migration inhibitory factor (MIF)Inhibitorinhibitorinhibit

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ISO-1 (Synonyms: MIF Antagonist)

Customer Review

ISO-1 Related Antibodies

13 Publications Citing Use of MCE ISO-1

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Complete Stock Solution Preparation Table

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