LYN-1604

Cat. No.: HY-101923: FAQs
Data Sheet Handling Instructions

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LYN-1604 is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC).

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (LYN-1604 dihydrochloride) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

LYN-1604 Chemical Structure

CAS No. : 2088939-99-3

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Other In-stock Forms of LYN-1604:

LYN-1604 hydrochloride

Other Forms of LYN-1604:

LYN-1604 hydrochloride In-stock
LYN-1604 dihydrochloride Get quote

3 Publications Citing Use of MCE LYN-1604

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Biological Activity
Purity & Documentation
References
Customer Review

Description

LYN-1604 is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC)^[1].

IC₅₀ & Target^[1]

ULK1

18.94 nM (EC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	IC₅₀	1.66 μM Compound: 8; LYN-1604	Antiproliferative activity against human MDA-MB-231 cells by MTT assay Antiproliferative activity against human MDA-MB-231 cells by MTT assay	[PMID: 29509411]

In Vitro

LYN-1604 is a potential ULK1 agonist (enzymatic activity=195.7% at 100 nM and IC₅₀=1.66 μM against MDA-MB-231 cells) ^[1].
LYN-1604 binds to wild-type ULK1 with a binding affinity in the nanomole range (K_D=291.4 nM) ^[1].
LYN-1604 (0.5, 1.0 and 2.0 μM) induces cell death via the ULK complex in MDA-MB-231 cells^[1].
LYN-1604 (0.5-2 μM, 24 hours) induces remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II in MDA-MB-231 cells^[1].
LYN-1604 induces ATG5-dependent autophagy via the ULK complex^[1].
LYN-1604 can also increase cleavage of caspase3 and induce apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0.5, 1.0 and 2.0 μM
Incubation Time:
Result:	Induced cell death. Autophagy ratio was increased in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0, 0.5, 1, and 2 μM
Incubation Time:	24 hours
Result:	Induced remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II.

In Vivo

LYN-1604 (low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg; intragastric administration once a day for 14 days) inhibits the growth of xenograft TNBC by targeting ULK1-modulated cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	24 female nude mice (BALB/c, 6-8 weeks, 20-22 g)^[1]
Dosage:	Low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg
Administration:	Intragastric administration; once a day for 14 days
Result:	Significantly inhibited the growth of xenograft MDA-MB-231 cells. The body weights of mice were stable. By the end of the experiment, the liver and spleen weight indexes of mice were slightly increased in parts of the groups, while the kidney weight index was not affected in all dose groups.

Molecular Weight

584.62

Formula

C₃₃H₄₃Cl₂N₃O₂

CAS No.

2088939-99-3

SMILES

ClC1=CC=C(C(CN2CCN(C(CN(CC(C)C)CC(C)C)=O)CC2)OCC3=CC=C(C=CC=C4)C4=C3)C(Cl)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (171.05 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7105 mL	8.5526 mL	17.1051 mL
5 mM	0.3421 mL	1.7105 mL	3.4210 mL
10 mM	0.1711 mL	0.8553 mL	1.7105 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. Discovery of a small molecule targeting ULK1-modulated cell death of triple negative breast cancer in vitro and in vivo. Chem Sci. 2017 Apr 1;8(4):2687-2701. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7105 mL	8.5526 mL	17.1051 mL	42.7628 mL
	5 mM	0.3421 mL	1.7105 mL	3.4210 mL	8.5526 mL
	10 mM	0.1711 mL	0.8553 mL	1.7105 mL	4.2763 mL
	15 mM	0.1140 mL	0.5702 mL	1.1403 mL	2.8509 mL
	20 mM	0.0855 mL	0.4276 mL	0.8553 mL	2.1381 mL
	25 mM	0.0684 mL	0.3421 mL	0.6842 mL	1.7105 mL
	30 mM	0.0570 mL	0.2851 mL	0.5702 mL	1.4254 mL
	40 mM	0.0428 mL	0.2138 mL	0.4276 mL	1.0691 mL
	50 mM	0.0342 mL	0.1711 mL	0.3421 mL	0.8553 mL
	60 mM	0.0285 mL	0.1425 mL	0.2851 mL	0.7127 mL
	80 mM	0.0214 mL	0.1069 mL	0.2138 mL	0.5345 mL
	100 mM	0.0171 mL	0.0855 mL	0.1711 mL	0.4276 mL

LYN-1604 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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