LYN-1604 dihydrochloride

Name: LYN-1604 dihydrochloride
Brand: MedChemExpress
SKU: HY-101923B
Rating: 5.0 (3 reviews)

Cat. No.: HY-101923B Purity: 99.64%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

LYN-1604 dihydrochloride is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC).

For research use only. We do not sell to patients.

LYN-1604 dihydrochloride Chemical Structure

CAS No. : 2310109-38-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 127	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 127	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 88	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of LYN-1604 dihydrochloride:

LYN-1604 hydrochloride In-stock
LYN-1604 Get quote

3 Publications Citing Use of MCE LYN-1604 dihydrochloride

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ULK1 ULK2

Biological Activity
Purity & Documentation
References
Customer Review

Description

LYN-1604 dihydrochloride is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC)^[1].

IC₅₀ & Target^[1]

ULK1

18.94 nM (EC₅₀)

In Vitro

LYN-1604 is a potential ULK1 agonist (enzymatic activity=195.7% at 100 nM and IC₅₀=1.66 μM against MDA-MB-231 cells) ^[1].
LYN-1604 binds to wild-type ULK1 with a binding affinity in the nanomole range (K_D=291.4 nM) ^[1].
LYN-1604 (0.5, 1.0 and 2.0 μM) induces cell death via the ULK complex in MDA-MB-231 cells^[1].
LYN-1604 (0.5-2 μM, 24 hours) induces remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II in MDA-MB-231 cells^[1].
LYN-1604 induces ATG5-dependent autophagy via the ULK complex^[1].
LYN-1604 can also increase cleavage of caspase3 and induce apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0.5, 1.0 and 2.0 μM
Incubation Time:
Result:	Induced cell death. Autophagy ratio was increased in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0, 0.5, 1, and 2 μM
Incubation Time:	24 hours
Result:	Induced remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II.

In Vivo

LYN-1604 (low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg; intragastric administration once a day for 14 days) inhibits the growth of xenograft TNBC by targeting ULK1-modulated cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	24 female nude mice (BALB/c, 6-8 weeks, 20-22 g)^[1]
Dosage:	Low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg
Administration:	Intragastric administration; once a day for 14 days
Result:	Significantly inhibited the growth of xenograft MDA-MB-231 cells. The body weights of mice were stable. By the end of the experiment, the liver and spleen weight indexes of mice were slightly increased in parts of the groups, while the kidney weight index was not affected in all dose groups.

Molecular Weight

657.54

Formula

C₃₃H₄₅Cl₄N₃O₂

CAS No.

2310109-38-5

Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.ClC1=CC=C(C(CN2CCN(C(CN(CC(C)C)CC(C)C)=O)CC2)OCC3=CC=C(C=CC=C4)C4=C3)C(Cl)=C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (76.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (76.04 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5208 mL	7.6041 mL	15.2082 mL
5 mM	0.3042 mL	1.5208 mL	3.0416 mL
10 mM	0.1521 mL	0.7604 mL	1.5208 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (281 KB) SDS (251 KB)

COA (254 KB) LCMS (275 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. Discovery of a small molecule targeting ULK1-modulated cell death of triple negative breast cancer in vitro and in vivo. Chem Sci. 2017 Apr 1;8(4):2687-2701. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.5208 mL	7.6041 mL	15.2082 mL	38.0205 mL
	5 mM	0.3042 mL	1.5208 mL	3.0416 mL	7.6041 mL
	10 mM	0.1521 mL	0.7604 mL	1.5208 mL	3.8021 mL
	15 mM	0.1014 mL	0.5069 mL	1.0139 mL	2.5347 mL
	20 mM	0.0760 mL	0.3802 mL	0.7604 mL	1.9010 mL
	25 mM	0.0608 mL	0.3042 mL	0.6083 mL	1.5208 mL
	30 mM	0.0507 mL	0.2535 mL	0.5069 mL	1.2674 mL
	40 mM	0.0380 mL	0.1901 mL	0.3802 mL	0.9505 mL
	50 mM	0.0304 mL	0.1521 mL	0.3042 mL	0.7604 mL
	60 mM	0.0253 mL	0.1267 mL	0.2535 mL	0.6337 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.