Lipoamide (Synonyms: (±)-α-Lipoamide; DL-Lipoamide; DL-6,8-Thioctamide)

Name: Lipoamide
Brand: MedChemExpress
SKU: HY-B1142
Rating: 5.0 (6 reviews)

Cat. No.: HY-B1142 Purity: 99.40%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Lipoamide ((±)-α-Lipoamide) is a monocarboxylic acid derivative of a neutral amide, formed by the condensation of the carboxyl group of lipoic acid and ammonia. Lipoamide protects against oxidative stress-mediated neuronal cell damage and also acts as a coenzyme to transfer acetyl groups and hydrogen during pyruvate deacylation. Lipoamide also stimulates mitochondrial biogenesis in adipocytes through the endothelial NO synthase-cGMP-protein kinase G signaling pathway.

For research use only. We do not sell to patients.

Lipoamide Chemical Structure

CAS No. : 940-69-2

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Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE Lipoamide

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Lipoamide (10-100 μM; 24 h) can reduce H₂O₂ or 6-OHDA-induced PC12 cell damage and apoptosis, reduce LDH leakage and Caspase-3 activity^[2].
Lipoamide (100 μM; 8 h) also upregulates the expression of Nrf2 in PC12 cells, promotes the nuclear translocation of Nrf2 and downstream gene expression, such as inducing the transcription of Nrf2-regulated antioxidant genes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	PC12 cells
Concentration:	10, 50, 100 μM
Incubation Time:	24 h; while cells were cultured with H₂O₂ or 6-OHDA for 5 h.
Result:	Effectively and dose-dependently rescued PC12 cells from cell injury caused by H₂O₂ or 6-OHDA. Significantly restored H₂O₂ or 6-OHDA-induced LDH leakage. Dose-dependently improved H₂O₂- or 6-OHDA-induced cell viability decrease.

Western Blot Analysis^[2]

Cell Line:	PC12 cells
Concentration:	10, 50, 100 μM
Incubation Time:	2, 4, 8 h; while cells were cultured with H₂O₂ or 6-OHDA for 5 h.
Result:	Improved the expression of nuclear Nrf2 remarkably, and the peak occurred at 4 h.

Molecular Weight

205.34

Formula

C₈H₁₅NOS₂

CAS No.

940-69-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N)CCCCC1SSCC1

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (243.50 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.67 mg/mL (3.26 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8700 mL	24.3499 mL	48.6997 mL
5 mM	0.9740 mL	4.8700 mL	9.7399 mL
10 mM	0.4870 mL	2.4350 mL	4.8700 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.38 mg/mL (11.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.38 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (243 KB) LCMS (110 KB)

Handling Instructions (2659 KB)

References

[1]. E S Arnér, et al. Efficient reduction of lipoamide and lipoic acid by mammalian thioredoxin reductase. Biochem Biophys Res Commun. 1996 Aug 5;225(1):268-74. [Content Brief]

[2]. Hou Y, et al. Lipoamide Ameliorates Oxidative Stress via Induction of Nrf2/ARE Signaling Pathway in PC12 Cells. J Agric Food Chem. 2019 Jul 24;67(29):8227-8234. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.8700 mL	24.3499 mL	48.6997 mL	121.7493 mL
DMSO	5 mM	0.9740 mL	4.8700 mL	9.7399 mL	24.3499 mL
	10 mM	0.4870 mL	2.4350 mL	4.8700 mL	12.1749 mL
	15 mM	0.3247 mL	1.6233 mL	3.2466 mL	8.1166 mL
	20 mM	0.2435 mL	1.2175 mL	2.4350 mL	6.0875 mL
	25 mM	0.1948 mL	0.9740 mL	1.9480 mL	4.8700 mL
	30 mM	0.1623 mL	0.8117 mL	1.6233 mL	4.0583 mL
	40 mM	0.1217 mL	0.6087 mL	1.2175 mL	3.0437 mL
	50 mM	0.0974 mL	0.4870 mL	0.9740 mL	2.4350 mL
	60 mM	0.0812 mL	0.4058 mL	0.8117 mL	2.0292 mL
	80 mM	0.0609 mL	0.3044 mL	0.6087 mL	1.5219 mL
	100 mM	0.0487 mL	0.2435 mL	0.4870 mL	1.2175 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Lipoamide Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipoamide940-69-2(±)-α-Lipoamide DL-Lipoamide DL-6,8-ThioctamideNO SynthaseNitric oxide synthasesNOSmonocarboxylic acid amidecoenzymeacetylhydrogenpyruvate deacylationInhibitorinhibitorinhibit

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Lipoamide (Synonyms: (±)-α-Lipoamide; DL-Lipoamide; DL-6,8-Thioctamide)

Customer Review

Lipoamide Related Antibodies

6 Publications Citing Use of MCE Lipoamide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NO Synthase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Lipoamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Lipoamide Related Classifications

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Your Recently Viewed Products:

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