Lucigenin (Synonyms: NSC-151912; L-6868)

Name: Lucigenin
Brand: MedChemExpress
SKU: HY-D0720
Rating: 5.0 (5 reviews)

Cat. No.: HY-D0720 Purity: 99.12%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O^2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm.

For research use only. We do not sell to patients.

Lucigenin Chemical Structure

CAS No. : 2315-97-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 20	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
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Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE Lucigenin

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Description

In Vitro

Preparation of Lucigenin working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lucigenin working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.

Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

510.50

Formula

C₂₈H₂₂N₄O₆

CAS No.

2315-97-1

Appearance

Solid

Color

Yellow to khaki

Emission (Em)

505

Excitation (Ex)

455

SMILES

C[N+]1=C2C=CC=CC2=C(C3=C4C=CC=CC4=[N+](C)C5=C3C=CC=C5)C6=C1C=CC=C6.O=N([O-])=O.O=N([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (122.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9589 mL	9.7943 mL	19.5886 mL
5 mM	0.3918 mL	1.9589 mL	3.9177 mL
10 mM	0.1959 mL	0.9794 mL	1.9589 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (259 KB) HNMR (272 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: Lucigenin is a chemiluminescent probe, it can be used to detect superoxide anion radicals in cells under alkaline condition with green fluorescence.
Method: For HCO₃⁻ transport assay.
1. Conidia and hyphae from the strains are treated at 28°C with 0.4 M mannitol in a solution of 50 mM NaHCO₃ for 2 h.
2. Wash cells and add 0.4 M mannitol to continue recovery (2 h; 28°C). A solution of only 0.4 M mannitol served as the control.
3. Incubate strains with Lucigenin (10 mM) and observed with a fluorescence microscope.

References

[1]. Li Y, et al. Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. J Biol Chem. 1998 Jan 23;273(4):2015-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9589 mL	9.7943 mL	19.5886 mL	48.9716 mL
	5 mM	0.3918 mL	1.9589 mL	3.9177 mL	9.7943 mL
	10 mM	0.1959 mL	0.9794 mL	1.9589 mL	4.8972 mL
	15 mM	0.1306 mL	0.6530 mL	1.3059 mL	3.2648 mL
	20 mM	0.0979 mL	0.4897 mL	0.9794 mL	2.4486 mL
	25 mM	0.0784 mL	0.3918 mL	0.7835 mL	1.9589 mL
	30 mM	0.0653 mL	0.3265 mL	0.6530 mL	1.6324 mL
	40 mM	0.0490 mL	0.2449 mL	0.4897 mL	1.2243 mL
	50 mM	0.0392 mL	0.1959 mL	0.3918 mL	0.9794 mL
	60 mM	0.0326 mL	0.1632 mL	0.3265 mL	0.8162 mL
	80 mM	0.0245 mL	0.1224 mL	0.2449 mL	0.6121 mL
	100 mM	0.0196 mL	0.0979 mL	0.1959 mL	0.4897 mL

Lucigenin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lucigenin2315-97-1NSC-151912 L-6868NSC151912NSC 151912L6868L 6868L-6868Fluorescent DyeReactive Oxygen SpeciesInhibitorinhibitorinhibit

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Lucigenin (Synonyms: NSC-151912; L-6868)

Customer Review

Lucigenin Related Antibodies

5 Publications Citing Use of MCE Lucigenin

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Lucigenin Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Lucigenin Related Classifications

Keywords:

Your Recently Viewed Products:

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