Melinamide (Synonyms: AC 223; DL-N-(α-Methylbenzyl)linoleamide)

Name: Melinamide
Brand: MedChemExpress
SKU: HY-101722
Rating: 4.5 (1 reviews)

Cat. No.: HY-101722: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Melinamide, an amide derivative of an unsaturated long-chain fatty acid, is an inhibitor of cholesterol absorption with an IC₅₀ of 20.9 μM.

For research use only. We do not sell to patients.

Melinamide Chemical Structure

CAS No. : 14417-88-0

Size	Price	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 220	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 220	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Melinamide, an amide derivative of an unsaturated long-chain fatty acid, is an inhibitor of cholesterol absorption with an IC₅₀ of 20.9 μM.

IC₅₀ & Target

IC50: 20.9 μM (cholesterol)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
THP-1	IC₅₀	5.2 μM Compound: 2b	Inhibition of LPS-induced TNFalpha production in human THP-1 cells incubated for 2 hrs followed by LPS stimulation and measured after 12 hrs by ELISA Inhibition of LPS-induced TNFalpha production in human THP-1 cells incubated for 2 hrs followed by LPS stimulation and measured after 12 hrs by ELISA	[PMID: 33479624]
THP-1	CC₅₀	95.8 μM Compound: 2b	Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33479624]

In Vitro

DL-Melinamide inhibits acyl CoA:cholesterol acyltransferase activity (ACAT) in the mucosal microsomes, with 50% inhibition occurring at approximately 0.5 μM. Kinetic studies indicate that DL-Melinamide is an uncompetitive inhibitor of acyl CoA:cholesterol acyltransferase. D-Melinamide is found to be a more effective inhibitor than L-Melinamide^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Melinamide is a new hypocholesterolaemic drug. Melinamide causes a substantial decrease of the enhanced intestinal ACAT activity in diabetic rats, but does not affect intestinal cholesterol esterase activity. Furthermore, marked improvement of hypercholesterolaemia in cholesterol-fed diabetic rats occurrs concomitantly with the drug treatment^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

383.61

Formula

C₂₆H₄₁NO

CAS No.

14417-88-0

Appearance

Oil

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\CCCCCCCC(NC(C1=CC=CC=C1)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (521.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6068 mL	13.0341 mL	26.0681 mL
5 mM	0.5214 mL	2.6068 mL	5.2136 mL
10 mM	0.2607 mL	1.3034 mL	2.6068 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (267 KB) SDS (252 KB)

COA (237 KB) HNMR (173 KB) LCMS (86 KB)

Handling Instructions (2659 KB)

References

[1]. Kusunoki J, et al. Effect of F-1394, a potent and selective inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), on esterification of cholesterol and basolateral secretion of cholesteryl ester in Caco-2 cells Nihon Yakurigaku Zasshi. 1997 Dec;110(6):357-65. [Content Brief]

[2]. Natori K, et al. Mechanism of the inhibition of cholesterol absorption by DL-melinamide: inhibition of cholesterol esterification. Jpn J Pharmacol. 1986 Dec;42(4):517-23. [Content Brief]

[3]. Matsubara K, et al. Cholesterol-lowering effect of N-(alpha-methylbenzyl)linoleamide (melinamide) in cholesterol-fed diabetic rats. Atherosclerosis. 1988 Aug;72(2-3):199-204. [Content Brief]

Animal Administration
^[3]

Rats: 10 diabetic rats are divided into two groups: a cholesterol-fed diabetic group and a melinamide-treated cholesterol-fed diabetic group. Five control rats are injected with citrate buffer only. The rats are fed each diet for 3 weeks after injection. The chol-fed DM group receives a diet (20 g/day) containing 1% cholesterol, 0.5% cholic acid and 5% lard. The melinamide-treated group receives the same diet but supplemented with 0.1% melinamide. Control rats are fed a standard chow (20 g/day). Following a 24-h fast the animals are killed. Blood is collected by aortic puncture and samples of small intestine are retained^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Kusunoki J, et al. Effect of F-1394, a potent and selective inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), on esterification of cholesterol and basolateral secretion of cholesteryl ester in Caco-2 cells Nihon Yakurigaku Zasshi. 1997 Dec;110(6):357-65. [Content Brief]

[2]. Natori K, et al. Mechanism of the inhibition of cholesterol absorption by DL-melinamide: inhibition of cholesterol esterification. Jpn J Pharmacol. 1986 Dec;42(4):517-23. [Content Brief]

[3]. Matsubara K, et al. Cholesterol-lowering effect of N-(alpha-methylbenzyl)linoleamide (melinamide) in cholesterol-fed diabetic rats. Atherosclerosis. 1988 Aug;72(2-3):199-204. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6068 mL	13.0341 mL	26.0681 mL	65.1704 mL
	5 mM	0.5214 mL	2.6068 mL	5.2136 mL	13.0341 mL
	10 mM	0.2607 mL	1.3034 mL	2.6068 mL	6.5170 mL
	15 mM	0.1738 mL	0.8689 mL	1.7379 mL	4.3447 mL
	20 mM	0.1303 mL	0.6517 mL	1.3034 mL	3.2585 mL
	25 mM	0.1043 mL	0.5214 mL	1.0427 mL	2.6068 mL
	30 mM	0.0869 mL	0.4345 mL	0.8689 mL	2.1723 mL
	40 mM	0.0652 mL	0.3259 mL	0.6517 mL	1.6293 mL
	50 mM	0.0521 mL	0.2607 mL	0.5214 mL	1.3034 mL
	60 mM	0.0434 mL	0.2172 mL	0.4345 mL	1.0862 mL
	80 mM	0.0326 mL	0.1629 mL	0.3259 mL	0.8146 mL
	100 mM	0.0261 mL	0.1303 mL	0.2607 mL	0.6517 mL

Melinamide Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melinamide14417-88-0AC 223 DL-N-(α-Methylbenzyl)linoleamideAC223AC-223OthersInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Melinamide (Synonyms: AC 223; DL-N-(α-Methylbenzyl)linoleamide)

Customer Review

Melinamide Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Melinamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Melinamide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report