NMDA (Synonyms: N-Methyl-D-aspartic acid)

Name: NMDA
Brand: MedChemExpress
SKU: HY-17551
Rating: 5.0 (19 reviews)

Cat. No.: HY-17551 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

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NMDA is a specific agonist for NMDA receptor mimicking the action of glutamate, the neurotransmitter which normally acts at that receptor.

For research use only. We do not sell to patients.

NMDA Chemical Structure

CAS No. : 6384-92-5

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Customer Review

Based on 19 publication(s) in Google Scholar

17 Publications Citing Use of MCE NMDA

: NMDA purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):827-834. [Abstract]; Representative western blots for SDHA and COX IV in the different groups of RGC-5 cells after 24 h (GAPDH is used as the reference protein).

: NMDA purchased from MedChemExpress. Usage Cited in: Neuroreport. 2017 May 24;28(8):444-450. [Abstract]; Western blot results further confirm the decrease in GDF10 expression induced by NMDA.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

NMDA is a specific agonist for NMDA receptor mimicking the action of glutamate, the neurotransmitter which normally acts at that receptor.

IC₅₀ & Target

Human Endogenous Metabolite

NMDA Receptor

Cellular Effect

Cell Line	Type	Value	Description	References
Oocyte	EC₅₀	22 μM Compound: NMDA	Activity at rat recombinant NR1/NR2B receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology Activity at rat recombinant NR1/NR2B receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology	[PMID: 18578474]
Oocyte	EC₅₀	23 μM Compound: NMDA	Activity at rat recombinant NR1/NR2C receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology Activity at rat recombinant NR1/NR2C receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology	[PMID: 18578474]
Oocyte	EC₅₀	75 μM Compound: NMDA	Activity at rat recombinant NR1/NR2A receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology Activity at rat recombinant NR1/NR2A receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology	[PMID: 18578474]
Oocyte	EC₅₀	8.3 μM Compound: NMDA	Activity at rat NR1/NR2D receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology Activity at rat NR1/NR2D receptor expressed in Xenopus oocytes assessed as effect on glutamate-induced current by two voltage clamp electrophysiology	[PMID: 18578474]

In Vitro

NMDA exerts a significant augmentation of the adrenal binding independently of the incubation temperature in a concentration-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NMDA (0.2 nmol) shows significant effects on MF, IF, IL, and EL, respectively, decreasing the mount and intromission frequencies, and shortening the intromission and ejaculation latencies. NMDA and AP-5 significantly, respectively, facilitates and inhibits the ejaculatory behavior during the copulation testing 30 min. Bilateral microinjection of NMDA into PVN significantly increases the baseline LSNA, the peaking increment of LSNA occurred within 5 min from the time of NMDA microinjected into PVN^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

147.13

Formula

C₅H₉NO₄

CAS No.

6384-92-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C[C@H](C(O)=O)NC

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (226.53 mM; Need ultrasonic)

DMSO : 10 mg/mL (67.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7967 mL	33.9836 mL	67.9671 mL
5 mM	1.3593 mL	6.7967 mL	13.5934 mL
10 mM	0.6797 mL	3.3984 mL	6.7967 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (6.80 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 36.67 mg/mL (249.24 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (253 KB) HNMR (269 KB) RP-HPLC (204 KB) MS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Xia JD, et al. Centrally mediated ejaculatory response via sympathetic outflow in rats: role of N-methyl-D-aspartic acid receptors in paraventricular nucleus. Andrology. 2016 Nov 16. [Content Brief]

[2]. Yoneda Y, et al. Enhancement of [3H]glutamate binding by N-methyl-D-aspartic acid in rat adrenal. Brain Res. 1987 Mar 17;406(1-2):24-31. [Content Brief]

[3]. Jiang L, et al. Decrease of growth and differentiation factor 10 contributes to neuropathic pain through N-methyl-D-aspartate receptor activation. Neuroreport. 2017 May 24;28(8):444-450. [Content Brief]

Kinase Assay ^[2]	Adrenal membranous homogenate suspensions are incubated with 10 nM [³H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [³H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [³H]GIu binding, K_d and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Thirty male rats are paired with different receptive females for a total of three times (once every 3 days) a week prior to the experiment, only the males that ejaculated at least three times during this period are included. After selecting the male rats with normal ejaculatory ability. Saline (100 nL), NMDA (0.20 nmol in 100 nL saline), and AP-5 (10.0 nmol in 100 nL saline) are adminitration into the bilateral PVN of each male rat in random order. After 5 min, the behavioral testing is performed and recorded as described above. Copulatory behaviors occur once a week and the entire experiment lasted 4 weeks. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Xia JD, et al. Centrally mediated ejaculatory response via sympathetic outflow in rats: role of N-methyl-D-aspartic acid receptors in paraventricular nucleus. Andrology. 2016 Nov 16. [Content Brief] [2]. Yoneda Y, et al. Enhancement of [3H]glutamate binding by N-methyl-D-aspartic acid in rat adrenal. Brain Res. 1987 Mar 17;406(1-2):24-31. [Content Brief] [3]. Jiang L, et al. Decrease of growth and differentiation factor 10 contributes to neuropathic pain through N-methyl-D-aspartate receptor activation. Neuroreport. 2017 May 24;28(8):444-450. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	6.7967 mL	33.9836 mL	67.9671 mL	169.9178 mL
	5 mM	1.3593 mL	6.7967 mL	13.5934 mL	33.9836 mL
	10 mM	0.6797 mL	3.3984 mL	6.7967 mL	16.9918 mL
	15 mM	0.4531 mL	2.2656 mL	4.5311 mL	11.3279 mL
	20 mM	0.3398 mL	1.6992 mL	3.3984 mL	8.4959 mL
	25 mM	0.2719 mL	1.3593 mL	2.7187 mL	6.7967 mL
	30 mM	0.2266 mL	1.1328 mL	2.2656 mL	5.6639 mL
	40 mM	0.1699 mL	0.8496 mL	1.6992 mL	4.2479 mL
	50 mM	0.1359 mL	0.6797 mL	1.3593 mL	3.3984 mL
	60 mM	0.1133 mL	0.5664 mL	1.1328 mL	2.8320 mL
H₂O	80 mM	0.0850 mL	0.4248 mL	0.8496 mL	2.1240 mL
H₂O	100 mM	0.0680 mL	0.3398 mL	0.6797 mL	1.6992 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

NMDA Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NMDA6384-92-5N-Methyl-D-aspartic acidiGluREndogenous MetaboliteIonotropic glutamate receptorsInhibitorinhibitorinhibit

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NMDA (Synonyms: N-Methyl-D-aspartic acid)

Customer Review

NMDA Related Antibodies

17 Publications Citing Use of MCE NMDA

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All iGluR Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

NMDA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

NMDA Related Classifications

Keywords:

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