Osajin (Synonyms: CID 95168; NSC 21565)

Name: Osajin
Brand: MedChemExpress
SKU: HY-N3125
Rating: 4.5 (1 reviews)

Cat. No.: HY-N3125 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera with antitumor, antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

Osajin Chemical Structure

CAS No. : 482-53-1

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1 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera with antitumor, antioxidant and anti-inflammatory activities.

IC₅₀ & Target

Apoptosis^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
ACHN	GI₅₀	12.13 μM Compound: 1, osajin	Growth inhibition of human ACHN cells after 21 hrs by MTT assay Growth inhibition of human ACHN cells after 21 hrs by MTT assay	[PMID: 17662606]
HCT-15	GI₅₀	9.65 μM Compound: 1, osajin	Growth inhibition of human HCT15 cells after 21 hrs by MTT assay Growth inhibition of human HCT15 cells after 21 hrs by MTT assay	[PMID: 17662606]
Hepatocyte	GI₅₀	> 500 μM Compound: 1, osajin	Growth inhibition of human hepatocytes after 21 hrs by MTT assay Growth inhibition of human hepatocytes after 21 hrs by MTT assay	[PMID: 17662606]
LOX IMVI	GI₅₀	10.98 μM Compound: 1, osajin	Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay	[PMID: 17662606]
MDA-MB-231	GI₅₀	11.34 μM Compound: 1, osajin	Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay	[PMID: 17662606]
NCI-H23	GI₅₀	11.97 μM Compound: 1, osajin	Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay	[PMID: 17662606]
PC-3	GI₅₀	12.32 μM Compound: 1, osajin	Growth inhibition of human PC3 cells after 21 hrs by MTT assay Growth inhibition of human PC3 cells after 21 hrs by MTT assay	[PMID: 17662606]
Vero	CC₅₀	11.44 μM Compound: Osajin	Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr	10.1101/2020.03.20.999730
Vero	IC₅₀	3.87 μM Compound: Osajin	Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr	10.1101/2020.03.20.999730

In Vitro

Osajin significantly decreases the viability of human NPC cells (TW076, CG1 and TW04 cells) in a dose-dependent manner. Osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress^[1]. Osajin exhibits growth inhibitory activity on six human cancer cell lines, including kidney, lung, prostate, breast, melanoma and colon cancer cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Osajin and pomiferin attenuates the myocardial dysfunction provoked by ischemia reperfusion. This is confirmed by an increase in both antioxidant enzyme values and total antioxidant activity. The cardioprotection provided by osajin and pomiferin treatment results from the suppression of oxidative stress and this correlates with improved ventricular function^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

404.46

Formula

C₂₅H₂₄O₅

CAS No.

482-53-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1C2=C(O)C(C/C=C(C)\C)=C3C(C=CC(C)(C)O3)=C2OC=C1C4=CC=C(O)C=C4

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥97.0%

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Data Sheet (276 KB) SDS (251 KB)

COA (252 KB)

Handling Instructions (2659 KB)

References

[1]. Huang TT, et al. Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin. PLoS One. 2011 Apr 12;6(4):e18308. [Content Brief]

[2]. Son IH, et al. Pomiferin, histone deacetylase inhibitor isolated from the fruits of Maclura pomifera. Bioorg Med Chem Lett. 2007 Sep 1;17(17):4753-5. [Content Brief]

[3]. Florian T, et al. Effects of prenylated isoflavones osajin and pomiferin in premedication on heart ischemia-reperfusion. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Jul;150(1):93-100. [Content Brief]

Cell Assay ^[1]	NPC cells are seeded in 96-well plates (5000/well) and allowed to adhere for 24 h. The medium is then substituted by a fresh one containing different concentrations (0.01–10 µM) of osajin for another 24 h. For the time-course assay, the incubation times with 5 µM of osajin are 6, 12, 24, 36 and 48 h. After incubation, 10 µl of 5 mg/ml of MTT is added to each well and incubated for 4 h at 37°C. Cell viability is determined using the MTT-based assay^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Rats^[1] The Wistar rats are divided into four groups. The first treatment group receives osajin (5 mg/kg/day in 0.5% Avicel); the second treatment group receives pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group receives only 0.5 Avicel; the last is an untreated control group. Biochemical indicator of oxidative damage-lipid peroxidation product malondialdehyde, antioxidant enzymes-superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium are evaluated. The effect of osajin and pomiferin on cardiac function, left ventricular end-diastolic pressure, left ventricular pressure and peak positive +dP/dt ischemia and reperfusion, also is examined^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Huang TT, et al. Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin. PLoS One. 2011 Apr 12;6(4):e18308. [Content Brief] [2]. Son IH, et al. Pomiferin, histone deacetylase inhibitor isolated from the fruits of Maclura pomifera. Bioorg Med Chem Lett. 2007 Sep 1;17(17):4753-5. [Content Brief] [3]. Florian T, et al. Effects of prenylated isoflavones osajin and pomiferin in premedication on heart ischemia-reperfusion. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Jul;150(1):93-100. [Content Brief]

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Osajin482-53-1CID 95168 NSC 21565CID95168CID-95168NSC21565NSC 21565NSC-21565ApoptosisInhibitorinhibitorinhibit

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Osajin (Synonyms: CID 95168; NSC 21565)

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