Phenolphthalein

Name: Phenolphthalein
Brand: MedChemExpress
SKU: HY-D0211
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0211 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Phenolphthalein is an acid-base indicator that is colorless in an acidic environment and pink in an alkaline environment. Phenolphthalein is genotoxic and potentially carcinogenic to mice. In addition, phenolphthalein is also a blood indicator.

For research use only. We do not sell to patients.

Phenolphthalein Chemical Structure

CAS No. : 77-09-8

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenolphthalein:

Phenolphthalein (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Phenolphthalein (2.5-10 μg/ml, 24 h; 1.25-15 μg/ml, 8 d) induces an increase in transformed cells in the Syrian hamster embryo (SHE) cell transformation assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Phenolphthalein (800 and 2400 mg/kg; i.g.; once daily for 26 weeks, 2400 mg/kg; p.o.; daily in the diet for 26 weeks) is genotoxic to p53+/- mice and causes proliferation of polychromatic erythrocytes with micronuclei^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

318.33

Formula

C₂₀H₁₄O₄

CAS No.

77-09-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC(C2=CC=C(O)C=C2)(C3=CC=C(O)C=C3)C4=C1C=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (157.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1414 mL	15.7071 mL	31.4141 mL
5 mM	0.6283 mL	3.1414 mL	6.2828 mL
10 mM	0.3141 mL	1.5707 mL	3.1414 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (7.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (281 KB) SDS (604 KB)

COA (260 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Fleischmann C, et al. Color indicator for supramolecular polymer chemistry: phenolphthalein-containing thermo- and pH-sensitive N-(Isopropyl)acrylamide copolymers and β-cyclodextrin complexation. Macromol Rapid Commun. 2013 Jul 12;34(13):1085-9. [Content Brief]

[2]. Higaki R S, et al. A study of the sensitivity, stability and specificity of phenolphthalein as an indicator test for blood[J]. Canadian Society of Forensic Science Journal, 1976, 9(3): 97-102.

[3]. Stoll R E, et al. Phenolphthalein and bisacodyl: assessment of genotoxic and carcinogenic responses in heterozygous p53 (+/−) mice and Syrian hamster embryo (SHE) assay[J]. Toxicological Sciences, 2006, 90(2): 440-450. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1414 mL	15.7071 mL	31.4141 mL	78.5353 mL
	5 mM	0.6283 mL	3.1414 mL	6.2828 mL	15.7071 mL
	10 mM	0.3141 mL	1.5707 mL	3.1414 mL	7.8535 mL
	15 mM	0.2094 mL	1.0471 mL	2.0943 mL	5.2357 mL
	20 mM	0.1571 mL	0.7854 mL	1.5707 mL	3.9268 mL
	25 mM	0.1257 mL	0.6283 mL	1.2566 mL	3.1414 mL
	30 mM	0.1047 mL	0.5236 mL	1.0471 mL	2.6178 mL
	40 mM	0.0785 mL	0.3927 mL	0.7854 mL	1.9634 mL
	50 mM	0.0628 mL	0.3141 mL	0.6283 mL	1.5707 mL
	60 mM	0.0524 mL	0.2618 mL	0.5236 mL	1.3089 mL
	80 mM	0.0393 mL	0.1963 mL	0.3927 mL	0.9817 mL
	100 mM	0.0314 mL	0.1571 mL	0.3141 mL	0.7854 mL