Quadrol (Synonyms: N,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine; EDTP)

Cat. No.: HY-B2149 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Quadrol is an immunostimulant and has been implicated as a potentially useful agent in accelerated wound healing.

For research use only. We do not sell to patients.

Quadrol Chemical Structure

CAS No. : 102-60-3

Size	Price	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
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Description

Quadrol is an immunostimulant and has been implicated as a potentially useful agent in accelerated wound healing.

In Vitro

Results show that viability of macrophages incubated with Quadrol at concentrations of 0.5 mM, 1 mM and 4mM are identical to that for controls. Viability, however, is reduced to 50% of control at concentrations of 16 mM to 32 mM. After four hours, at concentrations of 1 mM and 4 mM, Quadrol produces enhanced spreading of 88% and 80%, respectively, as compared to the control of 28%. Quadrol, at a concentration of 16 mM, shows reduced percentage of spreading of macrophages after four hours. Exposure of macrophages to 1.0 mM or 4.0 mM Quadrol concentrations enhances phagocytic activity, 41% and 57%, respectively, over that of the cells exposed to media alone (34%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

On day eight after the implantation, the amount of collagen in the implants of normal mice injected with Quadrol exceeds controls by more than 200% (p<0.025). By day 11, the collagen content increases to over 300% higher than controls (p<0.01) and by the end of two weeks after wounding, the time interval typically required for normal and complete wound healing, the collagen accumulated in the implants of the Quadrol-treated mice is about 50% above control (p<0.1). The accumulation of collagen in the implants of Quadrol treated STZ-diabetic mice about 100% above the untreated diabetic control on days 8 to 11. By day 14, the collagen deposition has increased to 200% above the controls (p<0.0 5)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

292.41

Formula

C₁₄H₃₂N₂O₄

CAS No.

102-60-3

Appearance

Oil

Color

Colorless to light yellow

SMILES

CC(O)CN(CC(O)C)CCN(CC(O)C)CC(O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (341.99 mM; Need ultrasonic)

DMSO : 100 mg/mL (341.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4199 mL	17.0993 mL	34.1986 mL
5 mM	0.6840 mL	3.4199 mL	6.8397 mL
10 mM	0.3420 mL	1.7099 mL	3.4199 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (246 KB) HNMR (287 KB) RP-HPLC (214 KB)

Handling Instructions (2659 KB)

References

[1]. Bhide MV, et al. In vitro stimulation of macrophages by quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine]. J Immunopharmacol. 1985;7(3):303-12. [Content Brief]

[2]. Bhide MV, et al. Promotion of wound collagen formation in normal and diabetic mice by quadrol. Immunopharmacol Immunotoxicol. 1988;10(4):513-22. [Content Brief]

Cell Assay ^[1]	The standard trypan blue exclusion test is performed to measure cell viability (10). Cells are exposed to increasing concentrations of Quadrol (0.5 mM to 32 mM) and incubated at 37°C for varying lengths of time before treatment with trypan blue^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	At the time of polytetrafluoroethylene (PTFE) tubing implantation, six test groups (3 streptozotocin-induced (STZ) diabetic and 3 non-diabetic) consisting of 5 to 7 animals per sampling time receive either diluent (control group) or Quadrol (10 mM) injected approximately 1 cm from the implantation site. The PTFE implants recovered from the mice after 2 to 14 days are stored frozen until processed and then analyzed by the HPLC method^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Bhide MV, et al. In vitro stimulation of macrophages by quadrol [N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine]. J Immunopharmacol. 1985;7(3):303-12. [Content Brief] [2]. Bhide MV, et al. Promotion of wound collagen formation in normal and diabetic mice by quadrol. Immunopharmacol Immunotoxicol. 1988;10(4):513-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.4199 mL	17.0993 mL	34.1986 mL	85.4964 mL
	5 mM	0.6840 mL	3.4199 mL	6.8397 mL	17.0993 mL
	10 mM	0.3420 mL	1.7099 mL	3.4199 mL	8.5496 mL
	15 mM	0.2280 mL	1.1400 mL	2.2799 mL	5.6998 mL
	20 mM	0.1710 mL	0.8550 mL	1.7099 mL	4.2748 mL
	25 mM	0.1368 mL	0.6840 mL	1.3679 mL	3.4199 mL
	30 mM	0.1140 mL	0.5700 mL	1.1400 mL	2.8499 mL
	40 mM	0.0855 mL	0.4275 mL	0.8550 mL	2.1374 mL
	50 mM	0.0684 mL	0.3420 mL	0.6840 mL	1.7099 mL
	60 mM	0.0570 mL	0.2850 mL	0.5700 mL	1.4249 mL
	80 mM	0.0427 mL	0.2137 mL	0.4275 mL	1.0687 mL
	100 mM	0.0342 mL	0.1710 mL	0.3420 mL	0.8550 mL

Quadrol Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Quadrol102-60-3N,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine EDTPOthersInhibitorinhibitorinhibit

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