2. GPCR/G Protein Neuronal Signaling
  3. Adrenergic Receptor
  4. Rauwolscine hydrochloride

Rauwolscine hydrochloride  (Synonyms: α-Yohimbine hydrochloride; Corynanthidine hydrochloride; Isoyohimbine hydrochloride)

Cat. No.: HY-12710A
COA Handling Instructions

Molarity Calculator

Rauwolscine hydrochloride is a potent and specific α2 adrenergic receptor antagonist with a Ki of 12 nM.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Rauwolscine) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Rauwolscine hydrochloride Chemical Structure

Rauwolscine hydrochloride Chemical Structure

CAS No. : 6211-32-1

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Other Forms of Rauwolscine hydrochloride:

Rauwolscine hydrochloride Related Antibodies

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  • Protocol

  • Purity & Documentation

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Description

Rauwolscine hydrochloride is a potent and specific α2 adrenergic receptor antagonist with a Ki of 12 nM.

IC50 & Target

Ki: 12 nM (α2 adrenergic receptor)[1]
In Vitro

[3H]Rauwolscine binding to α2 adrenergic receptor is reversible, stcreospccific, and saturable. [3H]Rauwolscine specifically labels both the high and low affinity states of the α2 adrenergic receptor in brain membranes[1]. [3H]Rauwolscine also behaves as a 5-HT1A receptor agonist and this conclusion is compatible with earlier functional studies, indicating that rauwolscine (as well as yohimbine) has agonistic properties at the level of 5-HT autoreceptors[2]. When using [3H]5-HT as a radioligand, rauwolscine is determined to have relatively high affinity for the human receptor (Ki human=14.3 nM, Ki rat=35.8 nM)[3]. Saturation studies shows that the affinity of [3H]Rauwolscine is similar in mouse, rat, rabbit, dog (2.33-3.03 nM) except man where it is significantly higher (0.98 nM) [4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rauwolscine hydrochloride Related Antibodies
Molecular Weight

390.90

Formula

C21H27ClN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.[H][C@]12C(NC3=C4C=CC=C3)=C4CCN1C[C@@]5([H])CC[C@H](O)[C@@H](C(OC)=O)[C@]5(C2)[H]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (31.98 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (12.79 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5582 mL 12.7910 mL 25.5820 mL
5 mM 0.5116 mL 2.5582 mL 5.1164 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Kinase Assay
[1]

Fresh bovine frontal cortex is incubated in triplicate with [3H]Rauwolscine (82 Ci/mM, diluted). Incubation is terminated by filtration under reduced pressure over filters, which are then rinsed with ice cold Tris-HCl buffer, dried overnight and added to disposable glass minivials containing 3.0 mL of a 95% Econofluor/5% Protosol solution. Samples are counted by liquid scintillation spectrometry with an efficiency of 32%. (-)- [3H]Epinephrine binding to bovine cortex membranes is conducted at 25°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.5582 mL 12.7910 mL 25.5820 mL 63.9550 mL
5 mM 0.5116 mL 2.5582 mL 5.1164 mL 12.7910 mL
10 mM 0.2558 mL 1.2791 mL 2.5582 mL 6.3955 mL
DMSO 15 mM 0.1705 mL 0.8527 mL 1.7055 mL 4.2637 mL
20 mM 0.1279 mL 0.6395 mL 1.2791 mL 3.1977 mL
25 mM 0.1023 mL 0.5116 mL 1.0233 mL 2.5582 mL
30 mM 0.0853 mL 0.4264 mL 0.8527 mL 2.1318 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Rauwolscine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rauwolscine6211-32-1α-YohimbineCorynanthidineIsoyohimbineAdrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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