2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Vitamin A

Vitamin A  (Synonyms: Vitamin A1; all-trans-Retinol)

Cat. No.: HY-B1342 Purity: ≥98.0%
SDS COA Handling Instructions

Molarity Calculator

Retinol is an endogenous metabolite.

For research use only. We do not sell to patients.

Vitamin A Chemical Structure

Vitamin A Chemical Structure

CAS No. : 68-26-8

Size Price Stock
5 mg USD 68 Ask For Quote & Lead Time
10 mg USD 110 Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of Vitamin A:

Vitamin A Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Retinol is an endogenous metabolite.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

It is found that contribution of hepatic microsomes (RDHs) to Retinol metabolism is greater than that of cytosol (ADHs), evidenced by higher Clint (Vmax/Km) of Retinol formation in microsomes than in cytosol[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Vitamin A Related Antibodies
In Vivo

The results show that compare with control (CON) rats, high-fat diet (HFD) significantly lowers basal level of Retinol in plasma, but markedly elevates basal levels of Retinol in kidney, adipose tissue and liver. The results show that Retinol absorption in HFD rats is faster than that in CON rats, evidenced by significantly shorter Tmax (3.0±0.0 h for HFD rats vs 5.8±1.1 h for CON rats, p<0.05)[1]. The plasma Retinol levels in methionine-choline deficient diet (MCD) rats are significantly lower than in the controls while the hepatic Retinol levels in MCD rats are markedly higher. The hepatic expression of Retinol-metabolizing enzymes and binding proteins (GRBP-I, ALDH1A1, and ALDH1A2) in MCD rats is significantly higher than that in control rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

286.45

Formula

C20H30O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

CC(/C=C/C=C(/C=C/C1=C(CCCC(C)1C)C)C)=C\CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (349.10 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4910 mL 17.4551 mL 34.9101 mL
5 mM 0.6982 mL 3.4910 mL 6.9820 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (558 KB)

COA (241 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

Activity of alcohol dehydrogenases (ADHs)/retinol dehydrogenases (RDHs) and retinal dehydrogenases (RALDHs) are assessed. Briefly, hepatic ADHs/RDHs and RALDHs activities are evaluated with Retinal formation in the presence of β-nicotinamide adenine dinucleotide (β-NAD+) and β-Nicotinamide adenine dinucleotide phosphate (β-NADP+), respectively. The incubation mixture consists of hepatic cytosol/microsomes (at the final concentration of 0.5 mg/mL), and a series concentrations of Retinol, 4.0 mM co-factor, 5 mM MgCl2, 91 μM butylated hydroxytoluene in a total volume of 500 μL. The reaction is initiated by the addition of Retinol pre-incubated for 5 min at 37°C. Following 15 min incubation, the reaction is quenched by extraction with equal volume of n-butanol/methanol, 95:5 (v:v) containing 0.005% butylated hydroxytoluene[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Wistar rats (male, aged 4 wk) are used in this study. Two groups of 6 rats are given free access to the following diets: the standard diet (control group) and the methionine-choline deficient diet (MCD) group. Each diet contains Retinol (1000 IU/100g). After 6 wk, the rats are sacrificed by exsanguination under isoflurane anesthesia after an overnight fast. Blood is collected in heparinized tubes, and plasma is separated for storage at -80°C. The liver, intestine, testes, and kidneys are removed, immediately frozen in liquid nitrogen, and stored at -80°C[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 3.4910 mL 17.4551 mL 34.9101 mL 87.2753 mL
5 mM 0.6982 mL 3.4910 mL 6.9820 mL 17.4551 mL
10 mM 0.3491 mL 1.7455 mL 3.4910 mL 8.7275 mL
15 mM 0.2327 mL 1.1637 mL 2.3273 mL 5.8184 mL
20 mM 0.1746 mL 0.8728 mL 1.7455 mL 4.3638 mL
25 mM 0.1396 mL 0.6982 mL 1.3964 mL 3.4910 mL
30 mM 0.1164 mL 0.5818 mL 1.1637 mL 2.9092 mL
40 mM 0.0873 mL 0.4364 mL 0.8728 mL 2.1819 mL
50 mM 0.0698 mL 0.3491 mL 0.6982 mL 1.7455 mL
60 mM 0.0582 mL 0.2909 mL 0.5818 mL 1.4546 mL
80 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0909 mL
100 mM 0.0349 mL 0.1746 mL 0.3491 mL 0.8728 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Vitamin A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vitamin A68-26-8Vitamin A1 all-trans-RetinolVitamin A 1Vitamin A-1Endogenous MetaboliteInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Vitamin A
Cat. No.:
HY-B1342
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (558 KB) Français - FR (558 KB) Deutsch - DE (558 KB) Norwegian - NO (558 KB) Español - ES (558 KB) Swedish - SV (558 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.