Rhodamine B (Synonyms: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine)

Name: Rhodamine B
Brand: MedChemExpress
SKU: HY-Y0016
Rating: 5.0 (9 reviews)

Cat. No.: HY-Y0016 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.

For research use only. We do not sell to patients.

Rhodamine B Chemical Structure

CAS No. : 81-88-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 44	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 44	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
1 g	USD 75	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Based on 9 publication(s) in Google Scholar

Other Forms of Rhodamine B:

Rhodamine B (Standard) Get quote

9 Publications Citing Use of MCE Rhodamine B

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Biological Activity
Purity & Documentation
References
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Description

Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay	[PMID: 28033541]
8505C	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human 8505C cells after 96 hrs by SRB assay Cytotoxicity against human 8505C cells after 96 hrs by SRB assay	[PMID: 28033541]
A2780	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A2780	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells after 96 hrs by SRB assay Cytotoxicity against human A2780 cells after 96 hrs by SRB assay	[PMID: 28033541]
A-375	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A549	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human A549 cells after 96 hrs by SRB assay Cytotoxicity against human A549 cells after 96 hrs by SRB assay	[PMID: 28033541]
FaDu	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HEK293	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HT-29	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HT-29	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay	[PMID: 28033541]
MCF7	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
MCF7	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay	[PMID: 28033541]
NIH3T3	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
NIH3T3	EC₅₀	> 30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay	[PMID: 28033541]

In Vitro

Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H₂O₂ (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

479.01

Formula

C₂₈H₃₁ClN₂O₃

CAS No.

81-88-9

Appearance

Solid

Color

Dark purple to black

Emission (Em)

610

Excitation (Ex)

546

SMILES

CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (260.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (208.76 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0876 mL	10.4382 mL	20.8764 mL
5 mM	0.4175 mL	2.0876 mL	4.1753 mL
10 mM	0.2088 mL	1.0438 mL	2.0876 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 5 mg/mL (10.44 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (277 KB) SDS (785 KB)

COA (254 KB) HNMR (266 KB) MS (71 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: Rhodamine B is a red fluorescent dye, it can be used to detect the biocatalytic membranes.
Method: For laccase stamp.

References

[1]. Tan D, et al. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0876 mL	10.4382 mL	20.8764 mL	52.1910 mL
	5 mM	0.4175 mL	2.0876 mL	4.1753 mL	10.4382 mL
	10 mM	0.2088 mL	1.0438 mL	2.0876 mL	5.2191 mL
	15 mM	0.1392 mL	0.6959 mL	1.3918 mL	3.4794 mL
	20 mM	0.1044 mL	0.5219 mL	1.0438 mL	2.6095 mL
	25 mM	0.0835 mL	0.4175 mL	0.8351 mL	2.0876 mL
	30 mM	0.0696 mL	0.3479 mL	0.6959 mL	1.7397 mL
	40 mM	0.0522 mL	0.2610 mL	0.5219 mL	1.3048 mL
	50 mM	0.0418 mL	0.2088 mL	0.4175 mL	1.0438 mL
	60 mM	0.0348 mL	0.1740 mL	0.3479 mL	0.8698 mL
	80 mM	0.0261 mL	0.1305 mL	0.2610 mL	0.6524 mL
	100 mM	0.0209 mL	0.1044 mL	0.2088 mL	0.5219 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine B81-88-9Basic Violet 10 Brilliant Pink B Rhodamine O TetraethylrhodamineBasic Violet10Basic Violet-10Fluorescent DyeInhibitorinhibitorinhibit

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Rhodamine B (Synonyms: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine)

Customer Review

Other Forms of Rhodamine B:

Rhodamine B Related Antibodies

9 Publications Citing Use of MCE Rhodamine B

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Rhodamine B Related Antibodies

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Complete Stock Solution Preparation Table

Rhodamine B Related Classifications

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