SEA0400

Name: SEA0400
Brand: MedChemExpress
SKU: HY-15515
Rating: 5.0 (18 reviews)

Cat. No.: HY-15515 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

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SEA0400 is a novel and selective inhibitor of the Na⁺-Ca²⁺ exchanger (NCX), inhibiting Na⁺-dependent Ca²⁺ uptake in cultured neurons, astrocytes, and microglia with IC₅₀s of from 5 to 33 nM.

For research use only. We do not sell to patients.

SEA0400 Chemical Structure

CAS No. : 223104-29-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 87	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 87	In-stock	Estimated Time of Arrival: December 31
Solid
2 mg	USD 61	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 92	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 495	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 825	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 18 publication(s) in Google Scholar

17 Publications Citing Use of MCE SEA0400

: SEA0400 purchased from MedChemExpress. Usage Cited in: Cell Signal. 2017 May 20;37:12-30. [Abstract]; HUVECs are incubated with the indicated concentrations of a second reverse-mode NCX inhibitor, SEA0400, for 30 min prior to stimulation with OAG (100 μM) for 5 min. ERK1/2 activation is assessed by Western blot.

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Protocol
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Description

IC₅₀ & Target

IC50: 5-33 nM (NCX)

In Vitro

SEA0400 inhibits Na⁺-dependent ⁴⁵Ca²⁺ uptake in cultured neurons, astrocytes, and microglia. IC₅₀ values of SEA0400 are 33 nM (neurons), 5.0 nM (astrocytes), and 8.3 nM (microglia)^[1]. SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation, and production of reactive oxygen species (ROS) in an extracellular Ca²⁺-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SEA0400 (3 mg/kg + 3 mg/kg/h for 2 h, i.v.) attenuates the infarct volume in the cerebral cortex and striatum, does not affect the mean the regional cortical blood flow in anesthetized rats^[1]. SEA0400 protects against the dopaminergic neurotoxicity (determined by dopamine levels in the midbrain and striatum, tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum, striatal dopamine release, and motor deficits) in MPTP-treated C57BL/6J mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

371.38

Formula

C₂₁H₁₉F₂NO₃

CAS No.

223104-29-8

Appearance

Solid

Color

White to khaki

SMILES

NC1=CC(OCC)=CC=C1OC2=CC=C(OCC3=CC(F)=CC=C3F)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (86.17 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6927 mL	13.4633 mL	26.9266 mL
5 mM	0.5385 mL	2.6927 mL	5.3853 mL
10 mM	0.2693 mL	1.3463 mL	2.6927 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.73 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (281 KB) SDS (392 KB)

COA (251 KB) LCMS (113 KB)

Handling Instructions (2659 KB)

References

[1]. Matsuda T, et al. SEA0400, a novel and selective inhibitor of the Na+-Ca2+ exchanger, attenuates reperfusion injury in the in vitro and in vivo cerebral ischemic models. J Pharmacol Exp Ther. 2001 Jul;298(1):249-56. [Content Brief]

[2]. Nashida T, et al. The specific Na(+)/Ca(2+) exchange inhibitor SEA0400 prevents nitric oxide-induced cytotoxicity in SH-SY5Y cells. Neurochem Int. 2011 Aug;59(1):51-8. [Content Brief]

[3]. Ago Y, et al. SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. Neuropharmacology. 2011 Dec;61(8):1441-51. [Content Brief]

Kinase Assay ^[1]	Na⁺-Ca²⁺ exchange activity is determined by assaying Na⁺-dependent ⁴⁵Ca²⁺ uptake as reported previously. Briefly, the cells are preincubated in Hanks' balanced saline solution (HBSS) for 20 min, and the medium is switched to HBSS containing ⁴⁵Ca²⁺ and incubated for 5 min. To increase intracellular Na⁺ concentration, 1 mM ouabain plus 20 μM monensin (for astrocytes and microglia) and 10 μM monensin (for neurons) are used. Monensin is added simultaneously with the isotope. Ouabain is added 5 min before monensin in astrocytes and microglia. SEA0400 and KB-R7943 are added 5 min before monensin and present during ⁴⁵Ca²⁺ uptake reaction. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay ^[1]	Cells, plated in 96-well plastic tissue culture plates, are incubated at 37°C for 30 min in normal or Ca²⁺-free HBSS containing 10 μM H2DCF-DA and 0.25 μg/mL Cremophor EL, and then rinsed twice with normal HBSS to remove excess dye. The cells are reperfused in normal HBSS for 1 h, and the conversion of H2DCF-DA to its fluorescent product dichlorofluorescein by ROS, presumably H₂O₂ and hydroxyl radical, is determined with excitation at 485 nm and emission at 535 nm using a Wallac Multilabel counter. ROS production is expressed as a percentage of control cells. The linearity and sensitivity of ROS assay are confirmed using H₂O₂ prior to the experiment. SEA0400 at the indicated concentrations is added 10 min before Ca²⁺ reperfusion and present until assay. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Male Sprague-Dawley rats, weighing 289 to 325 g, are anesthetized with 1 to 2% halothane. A catheter is inserted into the femoral artery and connected to a pressure transducer to record blood pressure. Regional cortical blood flow is measured by a laser Doppler flowmeter, with probe placement at 2 mm posterior and 6 mm lateral to the bregma. SEA0400 or its vehicle with an equivalent volume is i.v. injected at 3 mg/kg and then infused at 3 mg/kg/h for 2 h under normal conditions without MCA occlusion. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Matsuda T, et al. SEA0400, a novel and selective inhibitor of the Na+-Ca2+ exchanger, attenuates reperfusion injury in the in vitro and in vivo cerebral ischemic models. J Pharmacol Exp Ther. 2001 Jul;298(1):249-56. [Content Brief] [2]. Nashida T, et al. The specific Na(+)/Ca(2+) exchange inhibitor SEA0400 prevents nitric oxide-induced cytotoxicity in SH-SY5Y cells. Neurochem Int. 2011 Aug;59(1):51-8. [Content Brief] [3]. Ago Y, et al. SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. Neuropharmacology. 2011 Dec;61(8):1441-51. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6927 mL	13.4633 mL	26.9266 mL	67.3165 mL
	5 mM	0.5385 mL	2.6927 mL	5.3853 mL	13.4633 mL
	10 mM	0.2693 mL	1.3463 mL	2.6927 mL	6.7316 mL
	15 mM	0.1795 mL	0.8976 mL	1.7951 mL	4.4878 mL
	20 mM	0.1346 mL	0.6732 mL	1.3463 mL	3.3658 mL
	25 mM	0.1077 mL	0.5385 mL	1.0771 mL	2.6927 mL
	30 mM	0.0898 mL	0.4488 mL	0.8976 mL	2.2439 mL
	40 mM	0.0673 mL	0.3366 mL	0.6732 mL	1.6829 mL
	50 mM	0.0539 mL	0.2693 mL	0.5385 mL	1.3463 mL
	60 mM	0.0449 mL	0.2244 mL	0.4488 mL	1.1219 mL
	80 mM	0.0337 mL	0.1683 mL	0.3366 mL	0.8415 mL

SEA0400 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SEA0400223104-29-8SEA 0400SEA-0400Na+/Ca2+ ExchangerInhibitorinhibitorinhibit

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SEA0400

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SEA0400 Related Antibodies

17 Publications Citing Use of MCE SEA0400

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•Related Screening Libraries:

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Customer Review

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Complete Stock Solution Preparation Table

SEA0400 Related Classifications

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