2. Epigenetics Apoptosis
  3. MicroRNA Apoptosis
  4. SID 3712249

SID 3712249  (Synonyms: MiR-544 Inhibitor 1)

Cat. No.: HY-19731 Purity: 99.26%
SDS COA Handling Instructions

Molarity Calculator

SID 3712249 (MiR-544 Inhibitor 1) is a miR-544 biogenesis inhibitor. SID 3712249 binds directly to the precursor miRNA. SID 3712249 blocks production of the mature microRNA and decreases miR-544, HIF-1α, and ATM transcripts. SID 3712249 can be used in the research of cancers, such as breast cancer.

For research use only. We do not sell to patients.

SID 3712249 Chemical Structure

SID 3712249 Chemical Structure

CAS No. : 522606-67-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 92 In-stock
Solution
10 mM * 1 mL in DMSO USD 92 In-stock
Solid
5 mg USD 84 In-stock
10 mg USD 144 In-stock
25 mg USD 288 In-stock
50 mg USD 460 In-stock
100 mg USD 736 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

SID 3712249 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SID 3712249 (MiR-544 Inhibitor 1) is a miR-544 biogenesis inhibitor. SID 3712249 binds directly to the precursor miRNA. SID 3712249 blocks production of the mature microRNA and decreases miR-544, HIF-1α, and ATM transcripts. SID 3712249 can be used in the research of cancers, such as breast cancer[1].

IC50 & Target

MiR-544[1]
In Vitro

SID 3712249 (compound 1, 20 nM, 48 h) disrupts miR-544-mediated inhibition of BMI1 based on significant increases in the RFP/BFP ratio[1].
SID 3712249 (20 nM, 48 h) inhibits precursor but not mature miR-544 binding to BMI1 or mTOR3’-UTR[1].
SID 3712249 (20 nM, 48 h) results in accumulation of pre-miR-544 and a decrease in miR-544 levels in MCF10A cells[1].
SID 3712249 (20 nM, 5 days) induces apoptosis in MDA-MB-231 and MCF- 7 cells and has no effect on cell survival in normoxic conditions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SID 3712249 Related Antibodies

Apoptosis Analysis[1]

Cell Line: MDA-MB-231, MCF-7 cells
Concentration: 20 nM
Incubation Time: 5 days
Result: Induced apoptosis and has no effect on cell survival in normoxic conditions (flow cytometric analysis of Annexin V and PI stainning).
In Vivo

SID 3712249 (compound 1, 100 μL of 40 μM, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model[1].
SID 3712249 (20 nM, pre-treated GFP-labeled MDA-MB-231 cells, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231-GFP-luc tumor model[1]
Dosage: 100 μL of 40 μM
Administration: Intraperitoneal injection
Result: Inhibited tumor growth (evidenced by live animal bioluminescent imaging) with no overt side effects.
Decreased levels of miR-544, ATM, and HIF-1α and increased levels of mTOR (resected tumor samples).
Molecular Weight

323.40

Formula

C17H21N7
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=C2C(C=C(N3CCCC3)N=C2N)=C(C#N)C(N4CCCC4)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (51.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0921 mL 15.4607 mL 30.9215 mL
5 mM 0.6184 mL 3.0921 mL 6.1843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.26%

SDS (392 KB)

COA (251 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0921 mL 15.4607 mL 30.9215 mL 77.3037 mL
5 mM 0.6184 mL 3.0921 mL 6.1843 mL 15.4607 mL
10 mM 0.3092 mL 1.5461 mL 3.0921 mL 7.7304 mL
15 mM 0.2061 mL 1.0307 mL 2.0614 mL 5.1536 mL
20 mM 0.1546 mL 0.7730 mL 1.5461 mL 3.8652 mL
25 mM 0.1237 mL 0.6184 mL 1.2369 mL 3.0921 mL
30 mM 0.1031 mL 0.5154 mL 1.0307 mL 2.5768 mL
40 mM 0.0773 mL 0.3865 mL 0.7730 mL 1.9326 mL
50 mM 0.0618 mL 0.3092 mL 0.6184 mL 1.5461 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

SID 3712249 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SID 3712249522606-67-3MiR-544 Inhibitor 1SID3712249SID-3712249MicroRNAApoptosismiRNAHypoxiabreast cancermature microRNAATM-mTORMDA-MA-231GFP-lucInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
SID 3712249
Cat. No.:
HY-19731
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (392 KB) Français - FR (392 KB) Deutsch - DE (392 KB) Norwegian - NO (392 KB) Español - ES (392 KB) Swedish - SV (392 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.