Spermine (Synonyms: NSC 268508; Neuridine)

Name: Spermine
Brand: MedChemExpress
SKU: HY-B1777
Rating: 5.0 (4 reviews)

Cat. No.: HY-B1777 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Spermine (NSC 268508) functions directly as a free radical scabenger to protect DNA from free radical attack. Spermine has antiviral effects.

For research use only. We do not sell to patients.

Spermine Chemical Structure

CAS No. : 71-44-3

Size	Price	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Spermine:

4 Publications Citing Use of MCE Spermine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Spermine (NSC 268508) functions directly as a free radical scabenger to protect DNA from free radical attack. Spermine has antiviral effects.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	> 100 μM Compound: Spm, spermine	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO	IC₅₀	> 100 μM Compound: Spm, spermine	Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
Fibroblast	IC₅₀	> 500 μM Compound: Spermine	Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	> 500 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	14.6 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	14.9 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	356.7 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	422.1 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Huh-7	EC₅₀	> 50 μM Compound: Spermine	Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy	[PMID: 24177358]
L1210	IC₅₀	54.5 μM Compound: Spm, spermine	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]

In Vitro

Spermine (NSC 268508) at physiologically relevant concentrations inhibits ROS-induced DNA damage, with maximal inhibition observed at 1 to 2 mM. These concentrations are well within the estimates for the physiological concentrations of spermine^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

202.34

Formula

C₁₀H₂₆N₄

CAS No.

71-44-3

Appearance

<28°C Solid,>30°C Liquid

Color

Colorless to off-white

SMILES

NCCCNCCCCNCCCN

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (617.77 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9422 mL	24.7109 mL	49.4218 mL
5 mM	0.9884 mL	4.9422 mL	9.8844 mL
10 mM	0.4942 mL	2.4711 mL	4.9422 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (494.22 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (267 KB) SDS (476 KB)

COA (239 KB) HNMR (238 KB) MS (68 KB) GC (178 KB)

Handling Instructions (2659 KB)

References

[1]. Ha HC, et al. The natural polyamine spermine functions directly as a free radical scavenger. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11140-5. [Content Brief]

[2]. Mounce BC, et al. Polyamines and Their Role in Virus Infection. Microbiol Mol Biol Rev. 2017 Sep 13;81(4). pii: e00029-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.9422 mL	24.7109 mL	49.4218 mL	123.5544 mL
	5 mM	0.9884 mL	4.9422 mL	9.8844 mL	24.7109 mL
	10 mM	0.4942 mL	2.4711 mL	4.9422 mL	12.3554 mL
	15 mM	0.3295 mL	1.6474 mL	3.2948 mL	8.2370 mL
	20 mM	0.2471 mL	1.2355 mL	2.4711 mL	6.1777 mL
	25 mM	0.1977 mL	0.9884 mL	1.9769 mL	4.9422 mL
	30 mM	0.1647 mL	0.8237 mL	1.6474 mL	4.1185 mL
	40 mM	0.1236 mL	0.6178 mL	1.2355 mL	3.0889 mL
	50 mM	0.0988 mL	0.4942 mL	0.9884 mL	2.4711 mL
	60 mM	0.0824 mL	0.4118 mL	0.8237 mL	2.0592 mL
	80 mM	0.0618 mL	0.3089 mL	0.6178 mL	1.5444 mL
	100 mM	0.0494 mL	0.2471 mL	0.4942 mL	1.2355 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Spermine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spermine71-44-3NSC 268508 NeuridineNSC268508NSC-268508Endogenous MetaboliteInfluenza VirusInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Spermine (Synonyms: NSC 268508; Neuridine)

Customer Review

Other Forms of Spermine:

Spermine Related Antibodies

4 Publications Citing Use of MCE Spermine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Spermine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Spermine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report