2. Metabolic Enzyme/Protease GPCR/G Protein
  3. Endogenous Metabolite LPL Receptor
  4. Sphingosine-1-phosphate

Sphingosine-1-phosphate  (Synonyms: S1P)

Cat. No.: HY-108496 Purity: ≥99.0%
COA Handling Instructions

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Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca2+ as an extracellular ligand for G protein-coupled receptors. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids.

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Sphingosine-1-phosphate Chemical Structure

Sphingosine-1-phosphate Chemical Structure

CAS No. : 26993-30-6

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1 mg USD 236 In-stock
5 mg USD 470 In-stock
10 mg USD 658 In-stock
25 mg USD 1116 In-stock
50 mg USD 1540 In-stock
100 mg USD 2125 In-stock
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Based on 8 publication(s) in Google Scholar

Other Forms of Sphingosine-1-phosphate:

Sphingosine-1-phosphate Related Antibodies

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  • Purity & Documentation

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Description

Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca2+ as an extracellular ligand for G protein-coupled receptors[1]. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids[2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

S1P (1 μM) induces a significant Ca2+ releases in HEK293 cells under serum starvation conditions (1% FCS)[1].
In a functional Ca2+ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca2+ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca2+ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells[2].
In a functional Ca2+ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC50 values for gpr3 (EC50=29 nM), gpr6 (EC50=15 nM) and gpr12 (EC50=24 nM), rat gpr3 (EC50=68 nM),respectively in HEK293 cells[2].
S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and Gqi5, which allows for phospholipase C stimulation by Gi proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC50s = 8 and 11 nM, respectively)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sphingosine-1-phosphate Related Antibodies
Molecular Weight

379.47

Formula

C18H38NO5P
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

N[C@@H](CO[P](O)(O)=O)[C@H](O)/C=C/CCCCCCCCCCCCC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

1 M NaOH : 33.33 mg/mL (87.83 mM; Need ultrasonic)

Methanol : 3.85 mg/mL (10.15 mM; ultrasonic and warming and adjust pH to 3 with 1M HCl and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6353 mL 13.1763 mL 26.3525 mL
5 mM 0.5271 mL 2.6353 mL 5.2705 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  0.1% BSA in Saline

    Solubility: 0.5 mg/mL (1.32 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (240 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Methanol / 1 M NaOH 1 mM 2.6353 mL 13.1763 mL 26.3525 mL 65.8814 mL
5 mM 0.5271 mL 2.6353 mL 5.2705 mL 13.1763 mL
10 mM 0.2635 mL 1.3176 mL 2.6353 mL 6.5881 mL
1 M NaOH 15 mM 0.1757 mL 0.8784 mL 1.7568 mL 4.3921 mL
20 mM 0.1318 mL 0.6588 mL 1.3176 mL 3.2941 mL
25 mM 0.1054 mL 0.5271 mL 1.0541 mL 2.6353 mL
30 mM 0.0878 mL 0.4392 mL 0.8784 mL 2.1960 mL
40 mM 0.0659 mL 0.3294 mL 0.6588 mL 1.6470 mL
50 mM 0.0527 mL 0.2635 mL 0.5271 mL 1.3176 mL
60 mM 0.0439 mL 0.2196 mL 0.4392 mL 1.0980 mL
80 mM 0.0329 mL 0.1647 mL 0.3294 mL 0.8235 mL
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Sphingosine-1-phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sphingosine-1-phosphate26993-30-6S1PEndogenous MetaboliteLPL ReceptorLysophospholipid Receptorneuroprotectivesphingolipidlipid mediatorphospholipidintracellularsecond messengermobilizationca2+Inhibitorinhibitorinhibit

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