Suc-Leu-Leu-Val-Tyr-AMC

Name: Suc-Leu-Leu-Val-Tyr-AMC
Brand: MedChemExpress
SKU: HY-P1002
Rating: 5.0 (8 reviews)

Cat. No.: HY-P1002 Purity: 99.57%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Suc-Leu-Leu-Val-Tyr-AMC Chemical Structure

CAS No. : 94367-21-2

Size	Price	Stock	Quantity
1 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

8 Publications Citing Use of MCE Suc-Leu-Leu-Val-Tyr-AMC

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.

In Vitro

Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety^[1]. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

763.88

Formula

C₄₀H₅₃N₅O₁₀

CAS No.

94367-21-2

Appearance

Solid

Color

White to off-white

Emission (Em)

480

Excitation (Ex)

390

Sequence

Suc-Leu-Leu-Val-Tyr

Sequence Shortening

Suc-LLVY

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 20 mg/mL (26.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3091 mL	6.5455 mL	13.0911 mL
5 mM	0.2618 mL	1.3091 mL	2.6182 mL
10 mM	0.1309 mL	0.6546 mL	1.3091 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (283 KB) SDS (394 KB)

COA (260 KB) RP-HPLC (111 KB) MS (216 KB)

Handling Instructions (2659 KB)

References

[1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695–701. [Content Brief]

[2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19. [Content Brief]

[3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54. [Content Brief]

Kinase Assay
^[3]

Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695–701. [Content Brief]

[2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19. [Content Brief]

[3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3091 mL	6.5455 mL	13.0911 mL	32.7277 mL
	5 mM	0.2618 mL	1.3091 mL	2.6182 mL	6.5455 mL
	10 mM	0.1309 mL	0.6546 mL	1.3091 mL	3.2728 mL
	15 mM	0.0873 mL	0.4364 mL	0.8727 mL	2.1818 mL
	20 mM	0.0655 mL	0.3273 mL	0.6546 mL	1.6364 mL
	25 mM	0.0524 mL	0.2618 mL	0.5236 mL	1.3091 mL