TOOS (Synonyms: TOOS sodium salt)

Cat. No.: HY-15932 Purity: 99.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TOOS (TOOS sodium salt) is a highly water-soluble aniline derivative widely used in diagnostics and biological experiments. TOOS can be combined with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) to form a chromogenic system to measure oxidase activity. In the MBTH-TOOS chromogenic system, MBTH is catalytically oxidized to produce (-NH) free radicals, which react with TOOS to form colorless compounds. Furthermore, the colorless compound undergoes a disproportionation reaction to produce a blue-violet quinoid compound.

For research use only. We do not sell to patients.

TOOS Chemical Structure

CAS No. : 82692-93-1

Size	Price	Stock	Quantity
1 g	USD 35	In-stock	Estimated Time of Arrival: December 31
5 g	USD 100	In-stock	Estimated Time of Arrival: December 31
10 g	USD 150	In-stock	Estimated Time of Arrival: December 31
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Description

In Vitro

MBTH-TOOS chromogenic system^[1]
Cit-PtNPs oxidase as an example: Mix 50 μL Cit-PtNP, 250 μL 3 mM TOOS, and 200 μL 0.5 mM MBTH into 500 μL Tris-HCl buffer solution (10 mM, pH=8.0) and incubate at 40°C for 25 min. Subsequently, the absorbance was measured at 590 nm and the oxidase activity level was calculated.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

295.33

Formula

C₁₂H₁₈NNaO₄S

CAS No.

82692-93-1

Appearance

Solid

Color

White to off-white

SMILES

O=S(CC(O)CN(CC)C1=CC=CC(C)=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (338.60 mM; Need ultrasonic)

DMSO : ≥ 47 mg/mL (159.14 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3860 mL	16.9302 mL	33.8604 mL
5 mM	0.6772 mL	3.3860 mL	6.7721 mL
10 mM	0.3386 mL	1.6930 mL	3.3860 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Lin X, et al. Boosting the oxidase-like activity of platinum nanozyme in MBTH-TOOS chromogenic system for detection of trypsin and its inhibitor. Talanta. 2021 Nov 1;234:122647. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.3860 mL	16.9302 mL	33.8604 mL	84.6511 mL
	5 mM	0.6772 mL	3.3860 mL	6.7721 mL	16.9302 mL
	10 mM	0.3386 mL	1.6930 mL	3.3860 mL	8.4651 mL
	15 mM	0.2257 mL	1.1287 mL	2.2574 mL	5.6434 mL
	20 mM	0.1693 mL	0.8465 mL	1.6930 mL	4.2326 mL
	25 mM	0.1354 mL	0.6772 mL	1.3544 mL	3.3860 mL
	30 mM	0.1129 mL	0.5643 mL	1.1287 mL	2.8217 mL
	40 mM	0.0847 mL	0.4233 mL	0.8465 mL	2.1163 mL
	50 mM	0.0677 mL	0.3386 mL	0.6772 mL	1.6930 mL
	60 mM	0.0564 mL	0.2822 mL	0.5643 mL	1.4109 mL
	80 mM	0.0423 mL	0.2116 mL	0.4233 mL	1.0581 mL
	100 mM	0.0339 mL	0.1693 mL	0.3386 mL	0.8465 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

TOOS Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TOOS82692-93-1Biochemical Assay ReagentsAniline derivativesbiological diagnosticsblue-violet quinone typeInhibitorinhibitorinhibit

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