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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-P1002
    Suc-Leu-Leu-Val-Tyr-AMC
    		99.66%
    Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.
    Suc-Leu-Leu-Val-Tyr-AMC
  • HY-15902
    ABTS diammonium salt
    		99.86%
    ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
    ABTS diammonium salt
  • HY-D0090
    MQAE
    		99.84%
    MQAE is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. MQAE can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
    MQAE
  • HY-D0822
    CY3
    		99.87%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-151857
    TAMRA azide, 5-isomer
    		98.94%
    TAMRA azide, 5-isomer is a click chemical containing azide groups that can be used as a linker for the orange dye TAMRA. The azide group of TAMRA Azide, 5-isomer enables copper-catalyzed click chemical reactions with alkynes, DBCO, and BCN. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    TAMRA azide, 5-isomer
  • HY-15922
    Luminol
    		99.95%
    Luminol is a chemical that exhibits chemiluminescence with pKa values of 6.74 and 15.1. Luminol exhibits chemiluminescence (CL) at 425 nm λmax. Luminol is commonly used in forensics as a diagnostic tool for the detection of blood stains.
    Luminol
  • HY-D0831
    WST-8
    		99.67%
    WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
    WST-8
  • HY-Y0016
    Rhodamine B
    		≥98.0%
    Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
    Rhodamine B
  • HY-126561
    Green CMFDA
    		99.82%
    Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Green CMFDA
  • HY-D0717
    DAF-FM DA
    		98.32%
    DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).
    DAF-FM DA
  • HY-100041
    Bromobimane
    		99.31%
    Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.
    Bromobimane
  • HY-101899
    Monochlorobimane
    		99.86%
    Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.
    Monochlorobimane
  • HY-15925
    Nitro blue tetrazolium chloride
    		99.82%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-112641A
    AkaLumine hydrochloride
    		99.91%
    AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.
    AkaLumine hydrochloride
  • HY-D1434
    FM1-43
    FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.
    FM1-43
  • HY-101878
    Texas Red
    		99.72%
    Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
    Texas Red
  • HY-D1297
    ER-Tracker Green
    		98.02%
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
    ER-Tracker Green
  • HY-D0150
    Thiazole Orange
    		99.56%
    Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange has been widely used in biomolecular detection and staining of DNA/ RNA in gels and can be used for reticulocyte analysis. Thiazole orange generates a significant fluorescence enhancement and high quantum yield when it binds with nucleic acids, especially RNA. Thiazole orange can permeate living cell membranes. Thiazole orange can use UV light for detection, but can also be detected with blue light. The excitation and emission of Thiazole orange are λex = 510 nm (488 nm and 470 nm also show strong excitation) and λem = 527 nm, respectively.
    Thiazole Orange
  • HY-D0098
    Fluorescein-5-maleimide
    		99.05%
    Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.
    Fluorescein-5-maleimide
  • HY-136976
    WST-1
    		≥98.0%
    WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.
    WST-1
Cat. No. Product Name / Synonyms Application Reactivity