WST-8

Name: WST-8
Brand: MedChemExpress
SKU: HY-D0831
Rating: 5.0 (10 reviews)

Cat. No.: HY-D0831 Purity: 99.67%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

For research use only. We do not sell to patients.

WST-8 Chemical Structure

CAS No. : 193149-74-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 106	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 158	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE WST-8

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•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

In Vitro

The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×10⁵ cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

600.47

Formula

C₂₀H₁₃N₆NaO₁₁S₂

CAS No.

193149-74-5

Appearance

Solid

Color

White to yellow

SMILES

COC1=CC([N+]([O-])=O)=CC=C1[N+]2=NC(C3=CC=C(C=C3S(=O)([O-])=O)S(=O)(O[Na])=O)=NN2C4=CC=C(C=C4)[N+]([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 80 mg/mL (133.23 mM; ultrasonic and warming and heat to 60°C)

DMSO : 10 mg/mL (16.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6654 mL	8.3268 mL	16.6536 mL
5 mM	0.3331 mL	1.6654 mL	3.3307 mL
10 mM	0.1665 mL	0.8327 mL	1.6654 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.67 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.67 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (1.67 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (166.54 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (280 KB) SDS (476 KB)

COA (258 KB) HNMR (206 KB) LCMS (127 KB)

Handling Instructions (2659 KB)

References

[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.6654 mL	8.3268 mL	16.6536 mL	41.6341 mL
	5 mM	0.3331 mL	1.6654 mL	3.3307 mL	8.3268 mL
	10 mM	0.1665 mL	0.8327 mL	1.6654 mL	4.1634 mL
	15 mM	0.1110 mL	0.5551 mL	1.1102 mL	2.7756 mL
H₂O	20 mM	0.0833 mL	0.4163 mL	0.8327 mL	2.0817 mL
	25 mM	0.0666 mL	0.3331 mL	0.6661 mL	1.6654 mL
	30 mM	0.0555 mL	0.2776 mL	0.5551 mL	1.3878 mL
	40 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0409 mL
	50 mM	0.0333 mL	0.1665 mL	0.3331 mL	0.8327 mL
	60 mM	0.0278 mL	0.1388 mL	0.2776 mL	0.6939 mL
	80 mM	0.0208 mL	0.1041 mL	0.2082 mL	0.5204 mL
	100 mM	0.0167 mL	0.0833 mL	0.1665 mL	0.4163 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

WST-8 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WST-8193149-74-5WST8WST 8Fluorescent DyeInhibitorinhibitorinhibit

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WST-8

Customer Review

WST-8 Related Antibodies

10 Publications Citing Use of MCE WST-8

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

WST-8 Related Antibodies

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Complete Stock Solution Preparation Table

WST-8 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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