2. Metabolic Enzyme/Protease Apoptosis
  3. Endogenous Metabolite Apoptosis
  4. Ursocholic acid

Ursocholic acid, a bile acid present in mammalian bile, is converted to deoxycholic acid (UDC) by the mouse intestinal flora. Ursocholic acid acts as a gallstone dissolving agent in the liver through anti-apoptosis, anti-inflammatory, immunomodulatory, bile regulation, and coordinated changes in mitochondrial integrity and cell signaling, Ursocholic acid also has favorable effects on bones in patients with chronic cholestasis.

For research use only. We do not sell to patients.

Ursocholic acid Chemical Structure

Ursocholic acid Chemical Structure

CAS No. : 2955-27-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 132 In-stock
Solution
10 mM * 1 mL in DMSO USD 132 In-stock
Solid
5 mg USD 120 In-stock
10 mg USD 180 In-stock
25 mg USD 380 In-stock
50 mg USD 650 In-stock
100 mg USD 1100 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Ursocholic acid Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ursocholic acid, a bile acid present in mammalian bile, is converted to deoxycholic acid (UDC) by the mouse intestinal flora. Ursocholic acid acts as a gallstone dissolving agent in the liver through anti-apoptosis, anti-inflammatory, immunomodulatory, bile regulation, and coordinated changes in mitochondrial integrity and cell signaling, Ursocholic acid also has favorable effects on bones in patients with chronic cholestasis[1][2][3][4][5].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Ursocholic acid (1 μM, 10 μM, 100 μM and 1 mM, 24-72 h) has no deleterious effect on osteoblast survival at a lower concentration (100 μM), increases osteoblast differentiation treated with LCA (10 μM), and neutralizes the deleterious effects of bilirubin (50 μM)[5].
Ursocholic acid (1 μM, 10 μM and 100 μM, 7-28 days) increases osteoblast mineralization by 35% and 9% at days 21 and 28 at a dose of 100 μM[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ursocholic acid Related Antibodies

Cell Proliferation Assay[5]

Cell Line: osteoblast
Concentration: 1 μM, 10 μM, 100 μM and 1 mM
Incubation Time: 24-72 h
Result: Increased osteoblast proliferation at 1 μM and 10 μM concentrations. Significantly increased alkaline phosphatase (ALP) activity at 100 μM dose.
In Vivo

Ursocholic acid (10, 100 and 500 mg/kg, i.g.; once daily for 6 days) reduces significantly inflammatory cytokine levels and intestinal villus damage in Sprague-Dawley rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague‑Dawley rats[4].
Dosage: 10 mg/kg, 100 mg/kg and 500 mg/kg
Administration: i.g.; once a day for 6 days
Result: Increased jejunal villus length and weight-to-length ratio of intestinal tissue sections.
Reduced 5-FU-mediated mRNA expressions of TNF-α and IL-6 at a dose of 100 mg/kg and reduced MPO activity at doses of 100 and 10 mg/kg.
Molecular Weight

408.57

Formula

C24H40O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])[C@@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])C[C@H](O)[C@]12C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (244.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4476 mL 12.2378 mL 24.4756 mL
5 mM 0.4895 mL 2.4476 mL 4.8951 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

SDS (480 KB)

COA (251 KB) HNMR (141 KB) RP-HPLC (207 KB) MS (81 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4476 mL 12.2378 mL 24.4756 mL 61.1890 mL
5 mM 0.4895 mL 2.4476 mL 4.8951 mL 12.2378 mL
10 mM 0.2448 mL 1.2238 mL 2.4476 mL 6.1189 mL
15 mM 0.1632 mL 0.8159 mL 1.6317 mL 4.0793 mL
20 mM 0.1224 mL 0.6119 mL 1.2238 mL 3.0595 mL
25 mM 0.0979 mL 0.4895 mL 0.9790 mL 2.4476 mL
30 mM 0.0816 mL 0.4079 mL 0.8159 mL 2.0396 mL
40 mM 0.0612 mL 0.3059 mL 0.6119 mL 1.5297 mL
50 mM 0.0490 mL 0.2448 mL 0.4895 mL 1.2238 mL
60 mM 0.0408 mL 0.2040 mL 0.4079 mL 1.0198 mL
80 mM 0.0306 mL 0.1530 mL 0.3059 mL 0.7649 mL
100 mM 0.0245 mL 0.1224 mL 0.2448 mL 0.6119 mL
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Ursocholic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ursocholic acid2955-27-3Endogenous MetaboliteApoptosisInhibitorinhibitorinhibit

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