AAA-10 formic

Cat. No.: HY-145147A: FAQs
Data Sheet Handling Instructions

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AAA-10 formic is an orally active gut bacterial bile salt hydrolases (BSH) inhibitor, with IC₅₀s of 10 nM, 80 nM against B. theta rBSH and B. longum rBSH, respectively.

For research use only. We do not sell to patients.

AAA-10 formic Chemical Structure

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Other Forms of AAA-10 formic:

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1 Publications Citing Use of MCE AAA-10 formic

•Cell Host Microbe. 2024 Aug 21:S1931-3128(24)00290-7. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

AAA-10 formic is an orally active gut bacterial bile salt hydrolases (BSH) inhibitor, with IC₅₀s of 10 nM, 80 nM against B. theta rBSH and B. longum rBSH, respectively^[1].

IC₅₀ & Target

IC50: 10 nM (B. theta rBSH), 80 nM (B. longum rBSH)^[1]

In Vitro

AAA-10 (100 μM; 24 h) formic inhibits BSH activity in bacterial cultures, with IC₅₀s of 74 nM, 901 nM for Gram-negative and Gram-positive bacteria, respectively^[1].
AAA-10 (20 μM; 2 h) formic significantly inhibits deconjugation of glycochenodeoxycholic acid-d4 or taurocholic acid-d4 substrates of human feces^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AAA-10 (30 mg/kg; orally gavage daily for 5 days) formic decreases the abundances of deoxycholic acid (DCA) and lithocholic acid (LCA) in mice feces starting in day 2-5^[1].
AAA-10 (30 mg/kg) formic displays high colonic exposure and low gut permeability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10-11 weeks old male C57Bl/6J mice^[1]
Dosage:	30 mg/kg
Administration:	Orally gavaged once daily for 5 days
Result:	Decreased the abundances of DCA and LCA in mice feces starting in day 2-5. Displayed high colonic exposure and low gut permeability.

Molecular Weight

518.68

Formula

C₂₆H₄₃FO₇S

SMILES

C[C@H](CCC(CF)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC[C@]4([H])C[C@H](OS(=O)(O)=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C.O=CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (32.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9280 mL	9.6399 mL	19.2797 mL
5 mM	0.3856 mL	1.9280 mL	3.8559 mL
10 mM	0.1928 mL	0.9640 mL	1.9280 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (3.22 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (3.22 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (3.22 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Adhikari AA, et al. A Gut-Restricted Lithocholic Acid Analog as an Inhibitor of Gut Bacterial Bile Salt Hydrolases. ACS Chem Biol. 2021;16(8):1401-1412. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9280 mL	9.6399 mL	19.2797 mL	48.1993 mL
	5 mM	0.3856 mL	1.9280 mL	3.8559 mL	9.6399 mL
	10 mM	0.1928 mL	0.9640 mL	1.9280 mL	4.8199 mL
	15 mM	0.1285 mL	0.6427 mL	1.2853 mL	3.2133 mL
	20 mM	0.0964 mL	0.4820 mL	0.9640 mL	2.4100 mL
	25 mM	0.0771 mL	0.3856 mL	0.7712 mL	1.9280 mL
	30 mM	0.0643 mL	0.3213 mL	0.6427 mL	1.6066 mL

AAA-10 formic Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AAA-10AAA10AAA 10BacterialbacteriaHep-G2 cellsCaco-2 cellsmicefecesInhibitorinhibitorinhibit

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